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Citations for BioLegend :
1551 - 1600 of 3449 citations for Recombinant Human TNFRSF4 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... PE anti-human CD117 antibody (KIT; Biolegend, 313204) were used ...
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bioRxiv - Biophysics 2024Quote: ... and PE anti-human CD9 (BioLegend, cat. 312106).
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bioRxiv - Bioengineering 2022Quote: ... OT-I T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (BioLegend; San Diego, CA): TCR γ/δ (clone GL3) ...
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bioRxiv - Immunology 2021Quote: ... Any contaminating myeloid cells were removed from cultures prior to experimental setup by negative selection using a biotinylated anti-CD11b antibody (Biolegend, #101204) followed by subsequent Streptavidin MicroBeads (Miltenyi Biotec ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: NK cells were purified from spleen of wild type male mice by negative selection using magnetic beads and a cocktail of biotinylated antibodies: CD3e (145-2C11; BioLegend, 100304), CD4 (GK1.5 ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Biophysics 2023Quote: ... biotinylated anti-mouse CD3e (Cat# 100304) and FITC anti-mouse TCRb antibody (Cat# 109206) were purchased from BioLegend (San Diego, CA). Azide-PEG4-NHS ester (Cat# AZ103-100 ...
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bioRxiv - Bioengineering 2023Quote: 50 μm streptavidin-coated nanovials were reconstituted at a five-time dilution in Washing Buffer containing 270 nM (40 μg/mL) of biotinylated anti-CD63 antibody (Biolegend, 353018). Nanovials were incubated with antibodies for 30 minutes at room temperature on a rotator and washed three times as described above ...
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bioRxiv - Bioengineering 2023Quote: Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend, 368534) and anti-IFN-γ (R&D Systems ...
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2022Quote: ... cells were pre-treated with 2.5 μg/mL anti-CD16/32 (Fc Block; 2.4G2; BioLegend, San Diego, CA) for 15 minutes before continuing with surface staining ...
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bioRxiv - Immunology 2021Quote: ... 1×105-1×106 cells were stained after Fc-blocking with TruStain FcX (BioLegend, San Diego, CA, USA) and incubation with Zombie Aqua fixable viability dye (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were aspirated and cells were then treated with Fc block (clone 93, Ref: 101302, BioLegend, 1:1000) at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with a mix of Fc Block (anti-mouse CD16/CD32 mAb; clone 2.4G2, BioLegend) and fluorophore-conjugated anti-mouse mAbs.
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Cancer Biology 2019Quote: ... Fc receptors were blocked by incubation with 2.5 μg/mL anti-CD16/32 antibody (clone 93; 101320, BioLegend) in staining buffer (25 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.02% sodium azide) and resuspended in 25 µL 1:50 mouse FC block (TruStain FcX, BioLegend Cat# 101320), and left on ice for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocked cells were initially stained with FITC-conjugated anti-CD4 and BV421-conjugated anti-CD25 (102034; BioLegend) antibodies ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 50 ng/mL anti-Fc-γ III/II (CD16/32) receptor Ab (93, BioLegend) in 100 μl of FACS Buffer (1% FBS and 1 mM EDTA in PBS) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Microbiology 2022Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... FCS files were evaluated with the LEGENDplex™ Qognit 53790 Data Analysis Suite (Biolegend, San Diego, CA, USA). A five-parameter logistic curve-fitting method was used to calculate concentrations from Median Fluorescence Intensity values ...
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bioRxiv - Immunology 2023Quote: ... All samples were blocked with Fc-block anti-mouse CD16/32 antibody (clone: S17011E, cat. no. 156603, BioLegend). Single-cell suspensions were stained 30min at 4°C in the dark with PerCP/Cyanine5.5 anti-mouse Ly6G (clone:1A8 ...
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bioRxiv - Immunology 2023Quote: ... and unspecific binding of antibodies to Fc receptors was blocked by incubating the cells with TruStain FcXTM (Biolegend) diluted 1:50 in FACS buffer for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Tissue samples being directly analysed ex vivo were Fc-blocked by incubation with 5μg/ml αCD16/32 (BioLegend) for 10 minutes prior to viability and surface staining ...
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bioRxiv - Immunology 2023Quote: ... All samples were blocked with Fc-block anti-mouse CD16/32 antibody (clone: S17011E, cat. no. 156603, BioLegend). After the supernatant was discarded by centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then pooled into a single tube and incubated with Tru-Stain Fc blocking solution (Biolegend) for 10 mins at RT ...
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bioRxiv - Immunology 2023Quote: ... Individual samples were then combined and stained with intracellular antibodies in CyTOF buffer containing Fc receptor blocker (BioLegend) overnight at 4C ...
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bioRxiv - Microbiology 2023Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were incubated in mouse Fc block (anti-CD16/32) antibody (Biolegend, 101335, 1:300) and then stained with the appropriate antibodies in FACS Buffer as described previously [33] ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Biochemistry 2023Quote: ... Fc-receptors were first neutralized by incubating cells on ice for 5-10 minutes with TruStain FcX (BioLegend) at 2 μg/mL in 100 μL of blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors followed by staining with fluorochrome-conjugated anti–CD11b (1:200 dilution, BioLegend, no. 101226), anti–Ly6C (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor cells were washed with 1xPBS and blocked with 5% mouse Fc blocking reagent (Biolegend, San Diego, CA) in FACS buffer (1% FBS and 1 mM EDTA in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The pooled barcoded sample was stained for 10 minutes at room temperature with Fc receptor blocking solution (BioLegend) followed by 1h incubation with the antibody staining cocktail at room temprature ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6 or Rag2-/- mice were treated intranasally (i.n.) with 100 ng of recombinant IL-33 (Biolegend) each day for 5 consecutive days ...
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bioRxiv - Immunology 2022Quote: ... clones were screened by their loss of ability to produce IP-10 to recombinant IFNβ (Biolegend #581302).
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bioRxiv - Immunology 2021Quote: Timed-pregnant mice dams were intravenously injected with up to 10 μg of recombinant mouse cytokines (Biolegend) at gestation day 13.5 ...
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...