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Citations for BioLegend :
1551 - 1600 of 8462 citations for 7 CHLORO 2H PYRIDO 2 3 B 1 4 OXAZIN 3 4H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the cells were washed one more time with FACS washing buffer and then incubated with PE or Alexa647-conjugated FLAG tag antibody (PE, BioLegend Cat No ...
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bioRxiv - Cell Biology 2021Quote: ... the concentration of IL-2 in the supernatant was measured using an IL-2 ELISA kit (Biolegend).
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bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
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bioRxiv - Immunology 2023Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.
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bioRxiv - Immunology 2021Quote: ... and stained by intracellular staining for 30 min at 4 °C using a cocktail of the following antibodies: IFN-γ PE (Biolegend, Clone W18272D,1:500), TNF Percp-Cy5.5(Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421 ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspensions were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then washed with FACS buffer and incubated with the following Lineage antibodies for 30 min at 4°C: CD11b Biotin (BioLegend, Catalog No. 101204, 1/1000), CD11c Biotin (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for staining with the following antibodies for 20 min at 4° C: Ter119-FITC (BioLegend, Catalog No. 116205, 1/100), EpCAM-PE (BioLegend ...
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bioRxiv - Systems Biology 2023Quote: ... All iPSCs were stained in PBS with 5% FBS overnight at 4°C with the following antibodies and dilutions: Alexa Fluor® 647 anti-tubulin α (1:1000, Biolegend, #627908, clone 10D8), and Alexa Fluor ® 488 anti-Oct-4A (1:500 ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 20 minutes at 4 °C using the following antibodies: Brilliant Violet 605™ anti-human interferon-gamma (IFN-γ) (506542, Biolegend; 1 μL/well). After being stained ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... catalog number L100-L115-01) in PBS with 1% BSA and incubated the beads with 3.5 µg/ml biotinylated anti-CD81 antibody (BioLegend, clone Eat-2) at the room temperature for 40 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were fixed and permeabilized for intracellular staining of Granzyme B and FOXP3 using FluoroFix Buffer and Intracellular Staining Permeabilization Buffer (Biolegend catalog #421002) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell RNAseq data for the 7 PDA cell lines were generated as following: Cell lines were trypsinized and resuspended in Cell Staining Buffer (Biolegend, Cat. No. 420201) and incubated with proper Total Seq B antibodies using recommended protocol from Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
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bioRxiv - Immunology 2020Quote: ... IL-4-APC (clone MP4-25D2, BioLegend Cat# 500812, RRID:AB_315131), IL-17A-AlexaFluor488 (clone eBio64Dec17 ...
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bioRxiv - Neuroscience 2019Quote: ... and in 4 μl of APC anti-HA antibody (Biolegend). Bound GPC6 antibody were detected by incubating with a 1:200 dilution of goat anti-mouse Alexa-488 secondary antibody in 2% BSA for 45 min on ice ...
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bioRxiv - Immunology 2019Quote: ... 4 were analyzed with the following cocktail: CD4-BV650 (Biolegend), CD25-BV605(Biolegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 4 hours with Brefeldin A (Biolegend) and Monensin (Sigma Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and stained with CD11b-APC (M1/70, Biolegend), CD19-PE (HIB19 ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD62L PeCy7 (Cat#104418, Clone# MEL/4, BioLegend) on ice for 30 minutes (Supplemental Table 6) ...
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bioRxiv - Immunology 2020Quote: ... Tbet BV605 (clone 4B10, BioLegend, cat# 644817, dilution 4:100); Bcl6 PE/Dazzle (clone 7D1 ...
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bioRxiv - Immunology 2020Quote: ... ELISA MAX capture anti-IL-4 (previous antibodies from Biolegend) or capture TNFα (R&D systems ...
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bioRxiv - Genomics 2022Quote: ... 50 ng/mL of recombinant IL-4 (BioLegend, Cat# 574302) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and stained overnight at 4°C with avidin-FITC (BioLegend), anti-human/mouse CD117 (c-kit ...
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bioRxiv - Immunology 2022Quote: ... at 4°C for 15 minutes with antibody 3D12 (BioLegend) at 5 ng/μl ...
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bioRxiv - Microbiology 2022Quote: ... CD137 (4-1BB) PE (2.5 μg/mL, BioLegend Cat. 106106), and CD197 (CCR7 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then fixed in 1x Fixation Buffer (4% paraformaldehyde, BioLegend). Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 605 anti CTLA-4 clone UC10-4B9 (Biolegend), FITC anti-Foxp3 clone FJK-16s (eBioscience) ...
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bioRxiv - Immunology 2024Quote: ... and stained with anti-IFNγ–PE (clone 4 S.B3, BioLegend) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/ml of recombinant IL-4 (Cat# 574302, BioLegend), and 10 ng/ml of recombinant IL-13 (Cat# 575902 ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 uL APC conjugated anti-CD9 (clone H19a, Biolegend). Excess antibody was removed by two washes with 4°C PBS ...
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bioRxiv - Microbiology 2023Quote: ... 4°C) and stained with CD11b-APC (M1/70, Biolegend), CD19-PE (HIB19 ...
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bioRxiv - Immunology 2023Quote: ... 10 μg/mL anti-IL-4 mAb (11B11, BioLegend, 504122); Th2 condition ...
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bioRxiv - Bioengineering 2023Quote: ... Anti-SSEA-4 PE (2.5 µl/test, cat. 330406, Biolegend) and Anti-TRA-1-60 Alexa Fluor 647 (2.5 µl/test ...
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bioRxiv - Genomics 2023Quote: ... TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637, Biolegend) and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639 ...
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bioRxiv - Immunology 2023Quote: ... IL-4 (11B11) and IL-5 (TRFK5) were from Biolegend. Following surface and/or intracellular stain ...
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bioRxiv - Immunology 2023Quote: ... and 10 ng/mL of recombinant mouse IL-4 (BioLegend) diluted in PBS was added to wells cultured with the cytokine ...
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bioRxiv - Immunology 2024Quote: ... and IL-4 (20 ng/ml, Biolegend, San Diego, USA). Cells were allowed to differentiate for 5-7 days (DC-SIGN+ cells in the culture > 90 %).