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1501 - 1550 of 4555 citations for Recombinant Mouse Efna3 His tagged since 2019

• Interleukin-1 prevents SARS-CoV-2-induced membrane fusion to restrict viral transmission via induction of actin bundles

  Xu Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... mouse IL-1RA (769706) was purchased from BioLegend. IL-1β concentrations in supernatants from THP-1 and PBMCs were determined using ELISA kits ...
• Epigenetic control of CD8⁺ T cell tissue homing and tissue resident memory T cell precursors by the histone methyltransferase SUV39H1

  Guadalupe Suarez, et al., bioRxiv - Immunology 2024

  Quote: ... Brilliant Violet 711 anti-mouse CX3CR1 (SA011F11, Biolegend), BV711 CXCR3 (CXCR3-173 ...
• Forelimb motor recovery by modulating extrinsic and intrinsic signaling as well as neuronal activity after the cervical spinal cord injury

  Hirohide Takatani, et al., bioRxiv - Neuroscience 2024

  Quote: ... or mouse Tuj1 (1:1000, BioLegend, MMS- 435P) overnight at 4°C ...
• Coordinated differentiation of human intestinal organoids with functional enteric neurons and vasculature

  Charlie J. Childs, et al., bioRxiv - Bioengineering 2024

  Quote: ... mouse anti-TUBB3 1:200 (BioLegend Cat# 801201), sheep anti-SM22 1:500 (Novus Cat# AF7886) ...
• Cell of origin alters myeloid-mediated immunosuppression in lung adenocarcinoma

  Minxiao Yang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-mouse Ly6g-FITC (1:300, 127605, Biolegend), anti-mouse/human CD11b-PE/Dazzle 594 (1:120 ...
• Integrated multimodal cell atlas of Alzheimer’s disease

  Mariano I. Gabitto, et al., bioRxiv - Genomics 2024

  Quote: ... Beta Amyloid (1:1000, 6e10, Mouse, Biolegend 80303), Alpha-Synuclein (1:200 ...
• Epigenetic control of CD8⁺ T cell tissue homing and tissue resident memory T cell precursors by the histone methyltransferase SUV39H1

  Guadalupe Suarez, et al., bioRxiv - Immunology 2024

  Quote: ... PerCP/Cy5.5 anti-mouse TCR Vα2 (B20.1, Biolegend).
• Preeclampsia-specific immune cell network in placenta revealed by Cytometry by time of flight and single-cell RNA-seq

  Haiyi Fei, et al., bioRxiv - Immunology 2024

  Quote: ... APC-conjugated anti-mouse CD11b (101212, BioLegend, USA), FITC-conjugated anti-mouse Gr-1 (108406 ...
• Selective inflammation of the tumor microenvironment and invigorated T cell-mediated tumor control upon induced systemic inactivation of TREX1

  Emilija Marinkovic, et al., bioRxiv - Immunology 2024

  Quote: ... anti-mouse NK1.1 (APC conjugated, clone PK136, Biolegend), anti-mouse NKG2D (PE conjugated ...
• Omicron booster in ancestral strain vaccinated mice augments protective immunities against both the Delta and Omicron variants

  Liqiu Jia, et al., bioRxiv - Immunology 2022

  Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
• Phenotypic dissection of epithelial lineages and therapeutic manipulation of differentiation programs in human Adenoid Cystic Carcinomas (ACCs)

  Sara Viragova, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
• EGFR-initiated endocytosis of Wnt9a and Fzd9b is required for β-catenin signaling

  Nicole Nguyen, et al., bioRxiv - Cell Biology 2022

  Quote: ... EGFR knockout cells were also validated by testing for loss of tyrosine phosphorylation in response to a one-minute treatment with 50 ng/mL mouse EGF by immunoblotting with mouse monoclonal antibody (PY20, BioLegend, 309301).
• Csf1 from marrow adipogenic precursors is required for osteoclast formation and hematopoiesis in bone

  Leilei Zhong, et al., bioRxiv - Cell Biology 2022

  Quote: ... 45-5981-82), hamster anti-mouse CD48 APC (eBioscience, 17-0481-82) and rat anti-mouse CD150 PE-Cy7 (Biolegend, 115914). To examine macrophages ...
• Design of Cytotoxic T Cell Epitopes by Machine Learning of Human Degrons

  Nicholas L. Truex, et al., bioRxiv - Immunology 2023

  Quote: ... Antibody staining was performed at a dilution of 1:100 for 25 minutes at 4L°C in the presence of mouse Fc block (TruStain FcX™, anti-mouse CD16/32, BioLegend) in PBS containing 5% FBS ...
• Lack of the IFN-γ signal leads to lethal Orientia tsutsugamushi infection in mice with skin eschar lesions

  Yuejin Liang, et al., bioRxiv - Immunology 2024

  Quote: ... Some mice were intraperitoneally (i.p.) injected with Ultra-LEAF Purified anti-mouse NK-1.1 Antibody (clone#PK136; 200 µg/mouse, Biolegend, San Diego, CA) every other day ...
• Hematopoietic stem cell gene therapy improves outcomes in a clinically relevant mouse model of Multiple Sulfatase Deficiency

  Vi Pham, et al., bioRxiv - Neuroscience 2024

  Quote: ... PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody (103222) and APC anti-mouse CD3 Antibody (100236) antibodies (Biolegend, San Diego, CA) were utilized for the lymphoid populations ...
• CD4⁺ T cells drive corneal nerve damage but are dispensable for corneal epitheliopathy development in dry eye disease

  Alexia Vereertbrugghen, et al., bioRxiv - Immunology 2024

  Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
• DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctions

  Yuesong Hu, et al., bioRxiv - Biophysics 2023

  Quote: ... biotinylated anti-mouse CD3e (Cat# 100304) and FITC anti-mouse TCRb antibody (Cat# 109206) were purchased from BioLegend (San Diego, CA). Azide-PEG4-NHS ester (Cat# AZ103-100 ...
• Temperature-dependent intracellular crystallization of firefly luciferase in mammalian cells is suppressed by D-luciferin and stabilizing inhibitors

  Haruki Hasegawa, bioRxiv - Cell Biology 2024

  Quote: ... were from Santa Cruz Biotechnology. Mouse anti-β-tubulin (E7) was from the Hybridoma bank. Mouse anti-Nup153 (clone QE3, cat. 906201) was from Biolegend. Mouse anti-GAPDH (clone 6C5 ...
• Preeclampsia-specific immune cell network in placenta revealed by Cytometry by time of flight and single-cell RNA-seq

  Haiyi Fei, et al., bioRxiv - Immunology 2024

  Quote: ... PE-Cyanine7-conjugated anti-mouse Ly6G (E-AB-F1108H, elabscience, China) or intracellular staining for 488-conjugated anti-mouse IL-17A (506910, BioLegend, USA) was performed according to the manufacturer’s instructions.
• ART duration and immunometabolic state determine efficacy of DC-based treatment restoring functional HIV- specific CD8+ T cells in PLWH

  Marta Calvet-Mirabent, et al., bioRxiv - Immunology 2022

  Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
• Ran GTPase regulates non-centrosomal microtubule nucleation and is transported by actin waves towards the neurite tip

  Yung-An Huang, et al., bioRxiv - Cell Biology 2019

  Quote: ... Mouse anti-β-III-tubulin antibody TUJ1 (801202) and mouse anti-neurofilament monoclonal antibody SMI312 (837904) were from Biolegend (San Diego, CA). Mouse anti-α-tubulin antibody (DM1A ...
• Selective modulation of cell surface proteins during vaccinia infection: implications for immune evasion strategies

  Delphine M Depierreux, et al., bioRxiv - Microbiology 2021

  Quote: ... Cells were stained with a mouse anti-D8 monoclonal antibody AB1.1 (108) followed by PE-conjugated goat anti-mouse IgG (BioLegend, San Diego, U.S.A.). To assess human PM protein expression levels ...
• Enhanced immunogenicity of a synthetic DNA vaccine expressing consensus SARS-CoV-2 Spike protein using needle-free immunization

  Sawsan S Alamri, et al., bioRxiv - Immunology 2021

  Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
• Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4⁺ T cells through STAT1-related epigenetic mechanisms

  Jie Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... PE-conjugated anti- mouse IFN-γ (XMG1.2) and PerCP-Cy5.5-conjugated anti-mouse T-bet (4B10) were purchased from BioLegend (San Diego, CA).
• Targeting CD74-positive macrophages improves neoadjuvant therapy in cervical cancer as revealed by single-cell transcriptomics analysis

  Zixiang Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Single-cell suspension from mouse tumors or spleens were stained with Alexa Fluor 700 anti-mouse CD45 Antibody (Biolegend, USA, Cat#147716), APC anti-mouse F4/80 Antibody (Biolegend ...
• Fgf23 expression increases atherosclerotic plaque burden in male ApoE deficient mice

  Karolina Lindberg, et al., bioRxiv - Physiology 2024

  Quote: ... FGF23 was measured using the mouse FGF23 (c-term) ELISA (Immutopics) and IL-6 – using mouse interleukin-6 (IL-6) ELISA (BioLegend, CA, US). Serum 1,25-Dihydroxyvitamin D (1,25(OH)2D ...
• Lysine63-linked ubiquitin chains earmark GPCRs for BBSome-mediated removal from cilia

  Swapnil Rohidas Shinde, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-HA (mouse; clone 16B12; 901501; Biolegend;1:500), streptavidin-HRP (Pierce-21140;Thermo Scientific ...
• Primary mammary organoid model of lactation and involution

  Jakub Sumbal, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse monoclonal anti-keratin 8 (BioLegend, 904801; 1:200), and mouse monoclonal anti-β-casein (Santa Cruz ...
• The ESCRT protein CHMP5 restrains skeletal progenitor cell senescence by preserving endo-lysosomal-mitochondrial network

  Xianpeng Ge, et al., bioRxiv - Cell Biology 2020

  Quote: Mouse anti-beta-actin antibody (clone 2F1-1, Biolegend)
• Knock-in of labeled proteins into 5’UTR enables highly efficient generation of stable cell lines

  Faryal Ijaz, Koji Ikegami, bioRxiv - Cell Biology 2020

  Quote: ... HA.11 Epitope Tag (mouse mAb 16B12; 901502; Biolegend), Alexa fluorophore-conjugated secondary antibodies (Thermo ...
• Single-cell RNA sequencing unveils intestinal eosinophil development and specialization

  Alessandra Gurtner, et al., bioRxiv - Immunology 2021

  Quote: ... Armenian hamster anti-mouse CD80 (16-10A1, 104729 Biolegend) and rabbit antimouse p-NF-KB p65 (Ser536 ...
• Suppression of B-Cell Activation by Human Cord Blood-Derived Stem Cells (CB-SC) through the Galectin-9-Dependent Cell Contact Mechanism

  Wei Hu, et al., bioRxiv - Immunology 2021

  Quote: ... BV421-conjugated mouse anti-human CD34 (Biolegend, CA, USA), PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific ...
• The feedback regulator Nord controls Dpp/BMP signaling via extracellular interaction with Dally in the Drosophila wing

  Takuya Akiyama, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Mouse anti-HA (1:1,000, 16B12, MMS-101P, BioLegend) and mouse anti-Myc (1:1,000 ...
• Activation of Sarm1 produces cADPR to increase intra-axonal calcium and promote axon degeneration in CIPN

  Yihang Li, et al., bioRxiv - Neuroscience 2021

  Quote: ... and incubated with mouse anti-Tuj1 (1:500; BioLegend) overnight at 4°C ...
• Global inflammatory response in in vitro organ cultured testes using single-cell RNA-sequencing

  Takahiro Suzuki, et al., bioRxiv - Molecular Biology 2021

  Quote: ... and PE-conjugated anti-mouse IL1a antibody (clone:ALF161, BioLegend) containing 500 μl FACS buffer ...
• CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization

  Ying Feng, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Alexa Fluor® 647 anti-mouse CD24 Antibody (BioLegend) for HSA were added before cell sorting ...
• HLJ1 amplifies endotoxin–induced sepsis severity by promoting IL-12 heterodimerization in macrophages

  Wei-Jia Luo, et al., bioRxiv - Immunology 2022

  Quote: ... and Mouse IL-6 ELISA MAXTM Deluxe Set (Biolegend) according to the manufacturer’s protocol ...
• Tumor MHCII immunity requires in situ antigen presentation by cancer-associated fibroblasts

  Dimitra Kerdidani, et al., bioRxiv - Immunology 2020

  Quote: ... or isotypic controls (mouse IgG2a (Biolegend, Cat. No.361702) and mouse IgG1 (Abcam ...
• A novel subset of follicular helper-like MAIT cells has capacity for B cell help and antibody production in the mucosa

  Owen Jensen, et al., bioRxiv - Immunology 2021

  Quote: ... mouse anti-human IgD-BV421 (Clone IA6-2, Biolegend) and mouse anti-human PD1-APC (Clone EH1202H7 ...
• A novel subset of follicular helper-like MAIT cells has capacity for B cell help and antibody production in the mucosa

  Owen Jensen, et al., bioRxiv - Immunology 2021

  Quote: ... and mouse anti-human PD1-APC (Clone EH1202H7, Biolegend) all diluted 1:100 ...
• A novel subset of follicular helper-like MAIT cells has capacity for B cell help and antibody production in the mucosa

  Owen Jensen, et al., bioRxiv - Immunology 2021

  Quote: ... mouse anti-human CD161-AF488 (Clone NKR-P1A, Biolegend), mouse anti-human IgD-BV421 (Clone IA6-2 ...
• Pivotal involvement of the CX3CL1-CX3CR1 axis for the recruitment of M2-TAMs in skin carcinogenesis

  Yuko Ishida, et al., bioRxiv - Cancer Biology 2019

  Quote: ... rabbit anti-mouse Keratin1 pAbs (BioLegend, San Diego, CA), Cy3-conjugated donkey anti-rat IgG pAbs ...
• Low-dose IFN-γ induces tumor cell stemness in tumor microenvironment of non-small cell lung cancer

  Mengjia Song, et al., bioRxiv - Cancer Biology 2019

  Quote: ... and Cy3-conjugated anti-mouse IgG (BioLegend, 1:500) were used as secondary antibodies ...
• A Novel Tmem119-tdTomato Reporter Mouse Model for Studying Microglia in the Central Nervous System

  Chunsheng Ruan, et al., bioRxiv - Immunology 2019

  Quote: ... and mouse anti-CD11b-APC (1:80; #101212, BioLegend) for 30 minutes on ice in the dark ...
• Squamous trans-differentiation of pancreatic cancer cells promotes stromal inflammation

  Tim D.D. Somerville, et al., bioRxiv - Cancer Biology 2019

  Quote: ... anti-human/mouse E-Cadherin-AlexaFluor 647 (147304; BioLegend) and DAPI for 15 minutes ...
• Murine cytomegalovirus infection of melanoma lesions delays tumor growth by recruiting and re-polarizing monocytic phagocytes in the tumor

  Nicole A Wilski, et al., bioRxiv - Immunology 2019

  Quote: ... followed by anti-mouse IgG2b (clone RMG2b-1, BioLegend). Infected cells were identified using a Nikon eclipse Ti confocal microscope and analyzed with ImageJ (https://fiji.sc/).
• Gut-innervating nociceptor neurons protect against enteric infection by modulating the microbiota and Peyer’s patch microfold cells

  Nicole Y. Lai, et al., bioRxiv - Immunology 2019

  Quote: ... were coated with goat anti-mouse IgA-Biotin (BioLegend) and washed with 0.05% Tween/PBS ...
• Development of a Synthetic Poxvirus-Based SARS-CoV-2 Vaccine

  Flavia Chiuppesi, et al., bioRxiv - Immunology 2020

  Quote: ... Anti-mouse CD28 and CD49d antibodies (1μg/ml; BioLegend) were added as co-stimulation ...
• The Firre locus produces a trans-acting RNA molecule that functions in hematopoiesis

  Jordan P. Lewandowski, et al., bioRxiv - Immunology 2019

  Quote: ... PE/Cy7 anti-mouse CD127 (IL-7Rα) (Biolegend, 135014), Alexa Fluor 488 anti-mouse CD117 (c-Kit ...