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Citations for BioLegend :
1501 - 1550 of 1554 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... or a control isotype-matched antibody IgG (clone Poly4053) (BioLegend, R&D systems) were added at the primary phase (day 0 and 7 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with streptavidin-conjugated R-phycoerythrin (SA-PE) (Biolegend cat# 405204) and HLA-DR/DP/DQ conjugated to APC (Biolegend clone Tϋ39 cat#361714 ...
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bioRxiv - Genetics 2024Quote: Cell cycle analysis was performed using DAPI nucleic acid dye (Biolegend #422801). 1 day after passaging ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 2% neutral-buffered formalin diluted in FACS buffer following addition of Zombie Yellow fixable viability dye (BioLegend #423103, 1:200 in PBS).
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 μl of FACS Buffer and incubated with 1 μl of allophycocyanin (APC)-conjugated anti-CD46 antibody (BioLegend, clone TRA-2–10, 352405) for 30 min at 4°C with rotation ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for NK cells: (donors 1-2) anti-IFN-γ FITC (BioLegend catalog #506504, clone B27), anti-CD19 PerCP/Cy5.5 (BioLegend catalog #309504 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 million cells per sample were resuspended in CITE-seq staining buffer (2% BSA, .01% Tween in PBS) and incubated for 10 minutes with Fc receptor block (TruStain FcX, BioLegend and FcR blocking reagent, Miltenyi) to prevent antibody binding to Fc receptors ...
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bioRxiv - Developmental Biology 2021Quote: ... using PE-conjugated anti-human TRA-1-60-R antibody (Biolegend, catalog no. 330610).
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-human CCR2-biotin (FAB151B; R&D Systems/ 357224; BioLegend) in FACS buffer (HBSS ...
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bioRxiv - Immunology 2022Quote: ... and antibodies at a 1:200 dilution (anti-IFNγ, clone B27, Biolegend; anti-IL-17A, clone N49-653, BD Bioscience; anti-IL-22, clone 2G12A41, Biolegend; anti-IL-2, clone MQ1-17H12). Cells were washed and resuspended in FACS buffer and then analyzed on either an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems). For data output cell number and ELISA were normalized per 1,000 of each APC subset used for co-culture.
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bioRxiv - Cancer Biology 2023Quote: ... Biotin anti-mouse CD119 (IFN-γ R α chain) Antibody (BioLegend, catalog #112803, clone 2E2), PE Streptavidin (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-CD49f/APC (R&D, Cat.# FAB13501A) and anti-EpCAM/APC-Cy7 (Biolegend, Cat.# 118217) was used to identify Lin- stromal and epithelial subpopulations ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-CD49f/APC (R&D, Cat.# FAB13501A) and anti-EpCAM/APC-Cy7 (Biolegend, Cat.# 118217) were used to identify the lineage- (Lin- ...
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bioRxiv - Immunology 2024Quote: ... 100 µ L of biotinylated anti-IL12/23p40 clone 17.8 (Biolegend, 505301) in 1x dPBS + 0.1% BSA was added at a concentration of 1 µ g/mL and incubated for 1 hour at RT ...
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bioRxiv - Cell Biology 2020Quote: ... mice and cultured with M-CSF (20 µg/L, BioLegend, catalog number 576408) in vitro for 8-10 days to generate BMDMs ...
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Immunology 2020Quote: ... and homogenized lung were measured by ELISA according to the manufacturer’s instructions (Biolegend or R&D Systems). The levels of PGE2 were measured at the serum or the peritoneal lavage fluid of septic mice ...
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bioRxiv - Immunology 2023Quote: ... Commercial anti-RBD antibodies (mouse IgG1κ, clone 1035753, R&D systems or human IgG1κ, clone AM001414, BioLegend) and isotype controls (mouse IgG1κ ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... or 50ng/ml anti-human ICOS-L or 50ng/ml of the corresponding isotype control (Biolegend). After 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... PE anti-human TRAILR4 (DcR2) (R&DSystems, Clone 104918) or PE Mouse IgG1 κ Isotype Control (Biolegend, Clone MOPC-21) for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 10 ng/mouse of rmIL-12 (R&D) or 100 ng/mouse of rmIL-33 (Biolegend) alone or in combination in 50 µL directly in the tumor area twice a week from day 2 to day 26 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with primary antibodies anti-DC-SIGN/L-SIGN (Biolegend, cat. 845002, 1:500 dilution), anti-DC-SIGN (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... + 5 ml FBS + 2mM Ethylenediaminetetraacetic acid [EDTA]) with Human TruStain FcX™ Fc Receptor Blocking Solution (1:20; BioLegend) for 15 minutes at room temperature in foil ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... or with an anti-integrin ß4 antibody coupled to R-phycoerythrin (ITGß4-PE, 1:500, clone 58XB4, BioLegend, San Diego CA) for isolation of CTB ...
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bioRxiv - Microbiology 2021Quote: ... malayi were individually incubated at 37 °C in RPMI 1640 with L-glutamine with 125 nM DRAQ5 (Biolegend) for 24-48 hrs ...
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bioRxiv - Cell Biology 2024Quote: ... up to 5×104 CB-derived CD34+ HSPC were seeded into wells of 96-well plate in 100µl staining buffer (1xPBS, 0.5% BSA) and stained with 10µg/mL antibodies to CD34-PE (Clone 581, Biolegend) and CD45-PerCPCy5.5 (Clone QA17A19 ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Receptor surface expression levels were verified by flow cytometry using CXCR4- and CXCR7-specific mAbs (12G5, R&D Systems and 8F11-M16, BioLegend) on a BD FACS Fortessa cytometer (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: ... in PBS containing the primary antibodies at a 1:100 dilution: mouse anti-DC/L-SIGN (Biolegend, cat. 845002), rabbit anti-DC-SIGN (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... and 200 μl of primary antibody (1:200 anti-αSMA antibody, eBioscience, clone 1A4, efluor 570; 1:200 anti-CD31 antibody, Biolegend, clone MEC13.3 ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD11b expression MDS92 and MDS-L cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD11b (BioLegend) (#557743) ...
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bioRxiv - Bioengineering 2022Quote: ... at 0.5 μL/well for 5 hours and washed with PBS for detection of intracellular IFNγ using anti-hIFNγ APC antibody (Biolegend). The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II ...
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bioRxiv - Bioengineering 2023Quote: ... 50 µL aliquots of marrow cells were immunostained with 5 µL Alexa Fluor 488-conjugated rat IgG1 anti-mouse CD41 or APC-Cy7-conjugated rat IgG2b anti-mouse CD117 (BioLegend) and incubated at 4°C for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Immunology 2024Quote: ... CTLs were seeded on sapphire discs coated with poly-L-ornithine (0.1 mg/ml) and anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402) in flat specimen carriers (Leica) ...
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bioRxiv - Immunology 2024Quote: ... which were coated with poly-L-ornithine (0.1 mg/ml) and mouse anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402), for 10 min at RT (20°C ± 2°C ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Cancer Biology 2024Quote: 2*105 N87 and N87-R cells were incubated on ice for 30 min with PE anti-human HER2 antibody (Biolegend, clone 24D2, cat#324406), APC anti-human ABCG2 antibody (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... PMNs and platelets were visualized by applying 20 μl of platelet-specific FITC-labeled X488 antibody (Emfret, Eibelstadt, Germany) and 20 µl of PE-labeled anti-Ly6G (BioLegend #127608) with a catheter ...