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Citations for BioLegend :
1501 - 1550 of 10000+ citations for Anti CD25 SAP human Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Donor 1 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... CD69 levels were measured with APC-conjugated anti-human CD69 antibodies (Biolegend, 310909, clone FN50). TCR levels were measured with BV421-conjugated anti-human TCR antibodies (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... plates were coated with 2Lμg/ml of a mouse anti-human CD4 antibody (BioLegend, 300502) diluted in PBS overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The PE anti-human TGF-β Receptor II Antibody (clone W17055E, BioLegend, San Diego, CA) was used to detect dnTGFβRII expression on the CAR ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with plate bound anti-human CD3 Antibody (clone OKT3, 5ng/mL, #317326, Biolegend) and aCD28 (clone CD28.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed and permeabilized cells were stained with PE anti-human Ki-67 antibody (BioLegend #350504) diluted 1:10 in the BD Perm/Wash buffer from the kit for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend). Phenotyping was performed on a NovoCyte Advanteon flow cytometer and FACS was performed on a BD LSRII.
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-cMyc antibody and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: Analysis of CAR expression on transduced NK92 cells was done by staining with APC-conjugated anti-human CD314 (NKG2D) mAbs and anti-Flag Tag mAbs (Biolegend, USA). NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-cMyc antibody and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody (103222) and APC anti-mouse CD3 Antibody (100236) antibodies (Biolegend, San Diego, CA) were utilized for the lymphoid populations ...
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bioRxiv - Immunology 2022Quote: ... using human (BioLegend) and murine (PeproTech ...
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bioRxiv - Molecular Biology 2021Quote: ... using a LEGEND MAX™ Human IL-6 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BioLegend) with pre-coated plates ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... IL1beta ELISAs were performed using the ELISA MAX™ Deluxe Set Human IL1beta kit (437015, Biolegend) following the protocol provided by the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... anti c-kit-APC (Biolegend, 1:800) and anti sca1-PeCy7 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... anti-Neu5Gc antibody kit were from Biolegend, London ...
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bioRxiv - Immunology 2023Quote: ... anti-mCD117 (c-Kit) (2B8, BioLegend, 105816), anti-mCD200R3 (Ba13 ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... with 1 × 106 cells/mL 5000 rad irradiated PBMC + 50 U/mL IL-2 + 10 ng/mL IL-15 (both from NCI BRB Preclinical Biologics Repository) + 0.1 μg/mL each of anti-CD3 (Ultra-LEAF purified anti-human CD3 antibody clone OKT3; BioLegend, San Diego, CA) and anti-CD28 (Ultra-LEAF purified Anti-human CD28 antibody clone 28.2 ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... and stained overnight with Alexa 488-conjugated Alexa Fluor® 488 anti-tubulin β3 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Neuroscience 2022Quote: ... and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4) (BioLegend; Cat. No. 306514) for CRISPRa validation or 1:66 PE-Cy7 anti-human CD71 (TFRC ...
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bioRxiv - Neuroscience 2022Quote: ... for CRISPRa validation or 1:66 PE-Cy7 anti-human CD71 (TFRC) (BioLegend; Cat. No. 334112) for CRISPRi validation for 30 min in the dark ...
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bioRxiv - Cell Biology 2022Quote: Human cell chimerism after xenotransplantation was determined by staining with anti-mouse CD45-FITC (Biolegend, 103108) and anti-human CD45-APC (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies used in this study were Ultra-LEAF™ Purified anti-human CD63 H5C6 (Biolegend), Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499 ...
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bioRxiv - Molecular Biology 2020Quote: ... the UCB- and PB-MNCs were labeled with PE-conjugated anti-human CD34 (Biolegend, Cat.No.343506), APC-conjugated anti-human CD133 (Miltenyibiotec ...
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bioRxiv - Immunology 2019Quote: ... was used and for SCD1-retroviral transduced cell gating anti-human CD4 antibody (Clone: OKT4, Biolegend) was employed ...
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bioRxiv - Biophysics 2020Quote: ... USA) and PE-conjugated anti-human CD66a/c/e (dilution 1:40, #34303, BioLegend, CA, USA) in MB for 25 min at 600 rpm in a block thermomixer (Thermomixer comfort 5355 ...
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bioRxiv - Cancer Biology 2019Quote: ... All α-CD4 staining was performed with PE anti-human CD4 antibody clone RPA-T4 (Biolegend), unless otherwise stated ...
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bioRxiv - Cell Biology 2019Quote: THP-1 cells were labeled with mouse antibody anti-human CD18 (PE BioLegend or BV421 BD) (1 µl per 200 000 cells ...
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bioRxiv - Bioengineering 2021Quote: Cells were stained with the following antibodies: PE anti-human CD4 (clone RPA-T4, Biolegend 300507), FITC anti-human CD8a (clone RPA-T8 ...
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bioRxiv - Biochemistry 2020Quote: ... half the cells were stained for surface CD4 (anti-human CD4, Biolegend, conjugated directly to APC), fixed in formaldehyde ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-human BST-2 (clone RS38E, PE-Cy7-conjugated; Biolegend, San Diego, CA, USA) were also used as primary antibodies for cell-surface staining ...
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bioRxiv - Cancer Biology 2020Quote: ... and as previously described using the anti-human CD34 antibody11 (Clone 581; Biolegend, San Diego, CA).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were immediately stained with 5μL of either PE anti-human CD261 (DR4) (Biolegend, Clone DJR1), PE antihuman CD262 (DR5 ...
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bioRxiv - Cancer Biology 2022Quote: ... For blood-derived monocytes phenotyping, FITC-anti-human CD14 (Miltenyi, #130-110-576) antibody from Biolegend was also used.
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used for surface staining: PerCP/Cy5.5 anti-human CD4 (BioLegend, cat. 317428), PacificBlue anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with monoclonal mouse anti-human Lamp-1-AlexaFluor488 (BioLegend 328610, clone H4A3) at a final dilution of 1:500 for 1 hours ...
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bioRxiv - Immunology 2020Quote: ... Proteins were detected by immunoblotting using mouse anti-human CD9 (clone HI9a, BioLegend, dilution 1:1.000), mouse anti-human CD63 (clone TS63 ...
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Immunology 2022Quote: ... or 50ng/ml anti-human ICOS-L or 50ng/ml of the corresponding isotype control (Biolegend). After 60 min ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... Ultra-LEAF™ purified anti-human CD95 (FAS) blocking antibody (10 μg/ml, clone A16086F, Biolegend) or respective DMSO and H2O control were added prior to incubation ...
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bioRxiv - Immunology 2019Quote: ... at 0.1μg/ml each in 100μl total volume: AF647-conjugated anti-human/mouse CD11b (#101220, BioLegend), PE-conjugated anti-mouse Ly6C (#101220 ...
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bioRxiv - Genomics 2021Quote: ... 4 µl of the respective antibodies were used: APC anti-human SSEA-4 antibody (Biolegend, #330417) and PE anti-human TRA-1-60-R antibody (Biolegend ...