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Citations for BioLegend :
1501 - 1550 of 7320 citations for 7 2 Benzylamino ethyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Bioengineering 2021Quote: ... IL-6 was quantified using ELISA developed in the laboratory using biotin anti-rat IL-6 antibody (cat. # 517703) and purified (coating) anti-rat IL-6 antibody (cat. # 517701, Biolegend, San Diego, CA, USA). Coating antibody was diluted in 100 mM NaHCO3 ...
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bioRxiv - Microbiology 2021Quote: ... and intracellularly stained with anti-IL-6 (PE-Cy7, BioLegend), and anti-CXCL10 (PE ...
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bioRxiv - Immunology 2022Quote: ... Permeabilized cells were intracellularly stained with Bcl-6 (Biolegend 358512). Cells were acquired on Attune NxT Flow Cytometer and analyzed with Attune NxT software v 4.2.0.
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bioRxiv - Cancer Biology 2022Quote: ... anti-H-2Kb/H- 2Db (clone 28-8-6, Biolegend), anti-CD86 (clone GL-1 ...
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bioRxiv - Immunology 2021Quote: ... 40ng/ml rmGM-CSF and 40ng/ml rmIL-6 (BioLegend), with or without 50% E.G7-OVA supernatant ...
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bioRxiv - Immunology 2022Quote: ... TH17 skew media contained IL-6 (20ng/mL; BioLegend, 575704), IL-23 (10ng/mL ...
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bioRxiv - Neuroscience 2023Quote: ... TH17 skew media contained IL-6 (20ng/mL; BioLegend, 575704), IL-23 (10ng/mL ...
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bioRxiv - Immunology 2023Quote: ... ELISA MAX Deluxe Set Mouse IL-6 (BioLegend, Cat# 431315) and ELISA MAX Deluxe Set Mouse IL-1β (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse TotalSeq-A Hashtag antibody (4-6; BioLegend). The enriched samples were then stained with APC-labeled Streptavidin (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2021Quote: ... The cells were stimulated with 20μg/ml of a purified F(ab’)2 goat anti-mouse IgM (μ chain) antibody (BioLegend, Clone Poly21571, Cat# 157102) at 37°C for 10 seconds ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million cells for 30 min at 4 °C (Cat. No. 394601, 394603, 394605 and 394661, 394663, 394665, respectively, Biolegend, San Diego, CA, USA). After 3 washes with PBS with 0.05 % BSA respective cell suspensions were mixed prior to single-cell RNA library preparation ...
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bioRxiv - Immunology 2022Quote: ... 5 x 105 cells from each sample were stained for combinatorial hashing using oligonucleotide-conjugated Hashtag antibodies targeting the ubiquitous cell surface antigens Beta-2-Microglobulin and CD298 (Biolegend Totalseq A Hashtag antibodies). Hashed samples were pooled and stained together with a cocktail of 73 oligonucleotide-conjugated antibodies targeting cell surface antigens ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 3ml flow buffer (PBS with 2% FBS) at 300xg and stained with goat anti-mouse IgG-APC (BioLegend, clone Poly4053, catalog # 405308) and rat anti-mouse IgM-PE (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: Tumor cell lines and murine splenocytes were stained for flow cytometry assays in FACS buffer (1X PBS, 2 mM EDTA and 2c% FBS) with the following antibody panel: CD3-BV510 (BioLegend, clone 17A2, cat. 100233), NK1.1-PE-Cy7 (BioLegend ...
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bioRxiv - Immunology 2021Quote: IL-6 and IL-10 ELISAs (ELISA MAX Deluxe Set, Biolegend) were performed according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... ELISAs were used to measure IL-6 (Biolegend, San Diego, CA), GM-CSF (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... we used human RP10 media with combinations of rIL-6 (BioLegend), rIL-12 ...
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bioRxiv - Immunology 2020Quote: ... and stained intracellularly with anti-BCL-6 Alexa647 (IG191E/A8; Biolegend). Non-human primate LN suspensions and PBMC were stained with the same protocol ...
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bioRxiv - Immunology 2020Quote: ... interleukin (IL)-6 (10 ng/mL, Biolegend, San Diego, CA, USA), IL-1β (10 ng/mL ...
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bioRxiv - Genomics 2022Quote: ... and 20 ng/mL of recombinant IL-6 (BioLegend, Cat# 575702) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD41/CD61-PC7 (Clone: A2A9/6; Biolegend, 359812), Verified Reactivity ...
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Immunology 2022Quote: ... and further stained with anti-human Bcl-6 (PE, clone: 7D1, BioLegend) and anti-human Ki-67 (BV421 ...
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bioRxiv - Molecular Biology 2021Quote: ... Il-6 and IL-1β levels were quantified by ELISA (Biolegend kits).
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... H-2Kb/H-2Db-APC/Fire 750 (28-8-6; BioLegend, 114617), Streptavidin-APC/Cy7 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant mouse Oncostatin M and IL-6 (Biolegend, Cat #: 762802 & 575702 respectively).
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bioRxiv - Neuroscience 2023Quote: ... Four μg of Tau12 (N-terminal [epitope = amino acids 6-18], BioLegend), HT7 (mid-region [epitope = amino acids 159-163] ...
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bioRxiv - Cancer Biology 2023Quote: ... stained with antibodies to MHC-I (H2dB/H2kB; 28-8-6; Biolegend) and/or ab573(Evans et al. ...
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bioRxiv - Immunology 2023Quote: ... then washed 6 times followed by incubating with TMB substrate (Biolegend/421101) and stop solution ...
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bioRxiv - Pathology 2023Quote: ... and CD66b (mouse monoclonal, clone 6/40c; BioLegend, San Diego, CA, USA). Next ...
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bioRxiv - Immunology 2023Quote: ... C57BL/6 mice received 200 μg anti-Ly6G antibody (clone 1A8, Biolegend) via the i.p ...
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bioRxiv - Immunology 2022Quote: ... and antibodies at a 1:200 dilution (anti-IFNγ, clone B27, Biolegend; anti-IL-17A, clone N49-653, BD Bioscience; anti-IL-22, clone 2G12A41, Biolegend; anti-IL-2, clone MQ1-17H12). Cells were washed and resuspended in FACS buffer and then analyzed on either an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... with IL-6 (10ng/ml) (Peptrotech, USA) and BAFF (200ng/ml) (Biolegend, UK), supplemented with differing concentrations of leucine ...
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bioRxiv - Immunology 2021Quote: ... The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel (6-plex) was bought from BioLegend. Ultrapure water was obtained from an ELGA PURELAB flex 2 system with resistivity measured to at least 18.2 MΩ -cm.
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bioRxiv - Molecular Biology 2021Quote: ... PE anti-human IFNγ and anti-IL-6 antibodies were purchased from BioLegend. Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit α-keratin 10 and rabbit α-keratin 6 (Biolegend, San Diego, CA), rat α-keratin 8 (Developmental Studies Hybridoma Bank ...
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bioRxiv - Genomics 2021Quote: ... for hashing with TotalSeq-A anti-human Hashtag reagents (Supplementary Table 6, Biolegend) for 30 min at 4°C and following previously published protocol68 ...
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bioRxiv - Immunology 2021Quote: ... rat anti-mouse IL-6 or rat anti-mouse Ly6G (all from Biolegend), followed by fluorescence-conjugated secondary antibodies ...