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1451 - 1500 of 2682 citations for TMEM173 Human HEK293 Sumo His since 2019

• Spike-specific CXCR3⁺ T_FH cells play a dominant functional role in supporting antibody responses in SARS-CoV-2 infection and vaccination

  Jian Zhang, et al., bioRxiv - Immunology 2022

  Quote: ... FITC mouse anti-human PD-1 (EH12.2H7) (BioLegend, San Diego, CA, USA), and PE-eFluor 610 mouse anti-human CXCR5 (MU5UBEE ...
• A dynamic atlas of immunocyte migration from the gut

  Silvia Galván-Peña, et al., bioRxiv - Immunology 2022

  Quote: ... anti-mouse/human CD11b (Pacific Blue™, BioLegend, clone M1170, cat# 101224), anti-mouse F4/80 (APC-Cyanine® 7 ...
• Discovery of prevalent, clinically actionable tumor neoepitopes via integrated biochemical and cell-based platforms

  Hem Gurung, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend) and 2.5 ug/mL Ultra-LEAF anti-human CD28 (Biolegend) ...
• Investigating T cell Recruitment in Atherosclerosis using a novel Human 3D Tissue-Culture Model reveals the role of CXCL12 in intraplaque neovessels

  Laura Parma, et al., bioRxiv - Molecular Biology 2024

  Quote: ... the cell suspension was incubated with Human TruStain FcX (Biolegend, Cat. 422301) and afterward ...
• Overcoming brain-derived therapeutic resistance in HER2+ breast cancer brain metastasis

  Danyyl Ippolitov, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Recombinant human NRG1 was acquired from BioLegend (San Diego, CA, Cat. 551904). High-throughput drug screening assays were used with compounds sourced as indicated in Suppl ...
• Modelling Glioma Stem Cell-mediated Tumorigenesis Using Zebrafish PDX Systems

  Hema Priya Mahendran, et al., bioRxiv - Cancer Biology 2024

  Quote: FITC Anti-Human CD274 (B7-H1, PD-L1) Antibody (BioLegend; CA#393606)
• Involvement of the cellular prion protein in seeding and spreading of sarkosyl-derived fractions of Alzheimer’s disease in Prnp mutant mice and in the P301S transgenic tauopathy mice model

  Julia Sala-Jarque, et al., bioRxiv - Neuroscience 2024

  Quote: ... human tau-specific anti-Tau13 (1:200 dilution) (Biolegend, catalog no. 835201), murine tau-specific antibody anti-T49 (1:200 dilution ...
• De novo PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcription

  Luca Love, et al., bioRxiv - Microbiology 2024

  Quote: ... 5 µl of APC anti-human Ki-67 Antibody (BioLegend, CAT# 350513) in 100 µL of cell solution ...
• Defining the function of disease variants with CRISPR editing and multimodal single cell sequencing

  Yuriy Baglaenko, et al., bioRxiv - Genomics 2024

  Quote: ... followed by staining with the TotalSeq-A Human Universal Cocktail 1.0 (BioLegend) for 30 minutes on ice before washing and proceeding to single cell sorting ...
• Genome scale CRISPR screens identify actin capping proteins as key modulators of therapeutic responses to radiation and immunotherapy

  Nipun Verma, et al., bioRxiv - Genetics 2024

  Quote: ... For some experiments anti-human TIM3 antibody (Biolegend # 345009, 10 μg/ml) or anti-human PD1 antibody (BioXCell # BE0188 ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... FITC anti-mouse/human CD45R/B220 (clone RA3-6B2, BioLegend, 1:100), PE anti-mouse CD62L (clone MEL-14 ...
• Influenza A Virus Disruption of Dendritic Cell-Natural Killer Cell Crosstalk Impacts Activation of Helper and Cytotoxic T cell Subsets

  Connor A. Morson, et al., bioRxiv - Immunology 2024

  Quote: ... followed by staining with PE anti-human CD69 (clone: FN50; BioLegend®), or PE anti-human CD25 (clone ...
• Mitochondrial metabolism sustains CD8⁺ T cell migration for an efficient infiltration into solid tumors

  Luca Simula, et al., bioRxiv - Immunology 2024

  Quote: ... detected with the following anti-human antibodies: PE-anti-GrzB (Biolegend 372208), PE-Cy7-anti-Perforin (Biolegend 353315) ...
• Influenza A Virus Disruption of Dendritic Cell-Natural Killer Cell Crosstalk Impacts Activation of Helper and Cytotoxic T cell Subsets

  Connor A. Morson, et al., bioRxiv - Immunology 2024

  Quote: ... and Brilliant Violet 421™ anti-human CD209 (clone: 9E9A8 BioLegend®) antibodies ...
• Mutation-specific CAR T cells as precision therapy for IGLV3-21^R110 expressing high-risk chronic lymphocytic leukemia

  Florian Märkl, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The following markers were used: anti-human CD45-Pacific Blue (Biolegend, 368540), anti-mouse CD45 (30F11 ...
• Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survival

  Laura Zein, et al., bioRxiv - Cell Biology 2023

  Quote: ... rat anti-mouse/human Galectin 3 (BioLegend Cat# 125402, RRID:AB_1134238, 1:100), rabbit anti-TAX1BP1 (Sigma-Aldrich Cat# HPA024432 ...
• Longitudinal analysis of innate immune system in infants with perinatal HIV infection until 18 months of age

  Vinh Dinh, et al., bioRxiv - Immunology 2023

  Quote: ... 1 to 1.5 million PBMC were treated with Human TruStain FcX (Biolegend) to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen) ...
• CryoEM Structures of the Human HIV-1 Restriction Factor SERINC3 and Function as a Lipid Transporter

  Susan A. Leonhardt, et al., bioRxiv - Biophysics 2022

  Quote: ... and subjected to microvesicle depletion using anti-human CD45 magnetic beads (BioLegend) and a separation magnet (Miltenyi Biotec) ...
• Macrophage-targeted lipid nanoparticle delivery of microRNA-146a to mitigate hemorrhagic shock-induced acute respiratory distress syndrome

  Qinqin Fei, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... Cells were blocked with Human TruStain FcX (BioLegend, San Diego, CA, USA) for 20 minutes and stained for 20 minutes with fluorescently labeled antibodies ...
• MerTK mediates the immunologically silent uptake of alpha-synuclein fibrils by human microglia

  Marie-France Dorion, et al., bioRxiv - Cell Biology 2023

  Quote: Cells were blocked with Human TrueStain FcX and TrueStain Monocyte Blocker (Biolegend) and stained with the following antibodies ...
• Transgene-Free Ex Utero Derivation of A Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naïve PSCs

  Bernardo Oldak, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Mouse monoclonal anti human CD140a (PDFGR-a)-APC labeled (BioLegend Cat# 323512). FSC and SSC singlets were gated ...
• A unique human cord blood CD8⁺CD45RA⁺CD27⁺CD161⁺ T cell subset identified by flow cytometric data analysis using Seurat

  Julen Gabirel Araneta Reyes, et al., bioRxiv - Immunology 2023

  Quote: ... and Human TruStain FcX™ FcR block (Biolegend, San Diego, CA, USA) for 30 minutes at 4 °C ...
• Preclinical assessment of CAR-NK cell-mediated killing efficacy and pharmacokinetics in a rapid zebrafish xenograft model of metastatic breast cancer

  Nivedha Murali Shankar, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CAR-NK cells were loaded with human recombinant PD-L1- Fc (Biolegend) or ErBb2-Fc fusion proteins (R&D Systems ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 1µg/ml anti-human CD3 antibody (BioLegend, Clone OKT3, Cat. No. 317326) and 1µg/ml anti-human CD28 antibody ((BioLegend ...
• Combination of NY-ESO-1-TCR-T-cells coengineered to secrete SiRPα decoys with anti-tumor antibodies to augment macrophage phagocytosis

  Evangelos Stefanidis, et al., bioRxiv - Bioengineering 2023

  Quote: ... and subsequently stained with an anti-human Ki-67 (Ki-67, Biolegend) mAb at RT in the dark for 45min ...
• Combination of NY-ESO-1-TCR-T-cells coengineered to secrete SiRPα decoys with anti-tumor antibodies to augment macrophage phagocytosis

  Evangelos Stefanidis, et al., bioRxiv - Bioengineering 2023

  Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
• An aptamer-mediated base editing platform for simultaneous knock-in and multiple gene knockout for allogeneic CAR-T cells generation

  Immacolata Porreca, et al., bioRxiv - Molecular Biology 2023

  Quote: ... cells were stained with fluorophore-conjugated antibodies against human B2M (BioLegend, #316304), CD52 (BD BioSciences ...
• Trained immunity in human monocyte enhances myeloid-T-cell pathogenic crosstalk in Ankylosing Spondylitis

  Jinyi Zhao, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The following FACS anti-human antibodies were used: CD3 (clone OKT3; Biolegend); CD4 (clone RPA-T4 ...
• Reprogramming human B cells with custom heavy chain antibodies

  Geoffrey L. Rogers, et al., bioRxiv - Immunology 2023

  Quote: ... and 200 ng/mL recombinant human B cell-activating factor (BAFF, BioLegend).
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... Alexa Fluor® 647 anti-mouse/human GL7 antigen (Biolegend, Cat:144605)
• Combination of NY-ESO-1-TCR-T-cells coengineered to secrete SiRPα decoys with anti-tumor antibodies to augment macrophage phagocytosis

  Evangelos Stefanidis, et al., bioRxiv - Bioengineering 2023

  Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
• S100A8/A9 predicts triple-negative breast cancer response to PIM kinase and PD-1/PD-L1 inhibition

  Lauren R. Begg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rat anti-human CD4 conjugated to Alexa Fluor 700 (A161A1, Biolegend, 357417), eBioscience Fixable Viability Dye conjugated to eFluor 450 (Thermo Fisher Scientific ...
• CD8⁺ T-cell memory induced by successive SARS-CoV-2 mRNA vaccinations is characterized by clonal replacement

  Hiroyasu Aoki, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-human CD40 (final concentration of 0.5μg/mL, clone HB14, BioLegend)] with or without PepTivator SARS-CoV-2 Prot_S Complete (final concentration of 1 mg/mL ...
• Bioactive constituents of Verbena officinalis alleviate inflammation and enhance killing efficiency of natural killer cells

  Xiangdong Dai, et al., bioRxiv - Immunology 2022

  Quote: ... The cells were then stained with PerCP anti-human CD16 antibody (Biolegend) and APC mouse anti-human CD56 antibody (BD Biosciences ...
• EHD1-dependent traffic of IGF-1 receptor to the cell surface is essential for Ewing sarcoma tumorigenesis and metastasis

  Sukanya Chakraborty, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and live cells stained with PE-anti-human-IGF-1R (#351806; Biolegend) or PE-Mouse-IgG isotype control (#400112 ...
• GSK-3 inhibitor elraglusib enhances tumor-infiltrating immune cell activation in tumor biopsies and synergizes with anti-PD-L1 in a murine model of colorectal cancer

  Kelsey E. Huntington, et al., bioRxiv - Cancer Biology 2023

  Quote: ... APC/Cy7 anti-mouse/human CD11b clone: M1/70 (cat# 101226, BioLegend), anti-Cd27 Monoclonal Antibody (LG.7F9 ...
• MGA deletion leads to Richter’s transformation via modulation of mitochondrial OXPHOS

  Prajish Iyer, et al., bioRxiv - Cancer Biology 2023

  Quote: ... B220 (Pacific Blue™ anti-mouse/human CD45R/B220 [RA3-6B2], Biolegend) CD3 (APC/Cy7 anti-mouse CD3 [17A2] ...
• Distinct tumor architectures for metastatic colonization of the brain

  Siting Gan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 1:100 human TruStain FcX (Fc Receptor Blocking Solution) (BioLegend, Cat: 422301) and 1:100 TruStain FcX (anti-mouse CD16/32 ...
• Early β-amyloid accumulation in the brain is associated with peripheral T cell alterations

  Christoph Gericke, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were incubated with Fc receptor blocking antibodies (Human TruStain FcX, Biolegend) 1:20 in Cell Staining Buffer (CSB ...
• N-glycosylation engineering in chimeric antigen receptor T cells enhances anti-tumor activity

  Elien De Bousser, et al., bioRxiv - Immunology 2023

  Quote: ... CD123 and CD235 (MojoSort™ Human CD3 T cell selection kit, Biolegend) according to the manufacturer’s protocol ...
• Co-engagement of TIGIT⁺ immune cells to PD-L1⁺ tumours by a bispecific antibody potentiates T-cell response and tumour control

  Xiaopei Cui, et al., bioRxiv - Immunology 2023

  Quote: ... cells were collected and stained with BV510 anti human CD56 (Biolegend/318340), APC/CY7 anti human CD16 (Biolegend/302017) ...
• Mapping the functional impact of non-coding regulatory elements in primary T cells through single-cell CRISPR screens

  Celia Alda Catalinas, et al., bioRxiv - Genomics 2023

  Quote: ... Antibodies used were APC anti-human CD4 clone RPA-T4 (Biolegend, 300514), APC anti-human CD81 clone 5A6 (Biolegend ...
• The Sequence Basis for Selectivity of Ephrin-B2 Ligand for Eph Receptors and Pathogenic Henipavirus G Glycoproteins

  Krishna K. Narayanan, et al., bioRxiv - Microbiology 2023

  Quote: ... and anti-human IgG-allophycocyanin (APC) (clone M1310G05, 1/250 dilution, BioLegend) in PBS-BSA for 20 minutes at 4°C ...
• FAO-supported OxPhos leukemic stem cells are sensitive to cold

  Emmanuel Griessinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Human hematopoietic cells were first stained with Zombie Aqua™ (#423101, Biolegend) following the manufacturer’s instructions ...
• Endothelial cells are a key target of IFN-g during response to combined PD-1/CTLA-4 ICB treatment in a mouse model of bladder cancer

  Sharon L. Freshour, et al., bioRxiv - Cancer Biology 2023

  Quote: ... APC anti-mouse/human CD11b Antibody (BioLegend, catalog #101211, clone M1/70), PE/Cyanine7 anti-mouse CD11c Antibody (BioLegend ...
• Loss of IRF5 increases ribosome biogenesis leading to alterations in mammary gland architecture and metastasis

  Zarina Brune, et al., bioRxiv - Cancer Biology 2023

  Quote: Alexa Fluor® 647 anti-human/mouse CD49f (Biolegend, #313609, 1:100)
• CBFA2T3-GLIS2-dependent pediatric acute megakaryoblastic leukemia is driven by GLIS2 and sensitive to Navitoclax

  Mathieu Neault, et al., bioRxiv - Cancer Biology 2022

  Quote: ... 10 ng/mL human recombinant IL-3 (BioLegend, San Diego, CA, USA) and antibiotics ...
• Bile acids mediate liver-bone marrow crosstalk

  Daniele Vitale, et al., bioRxiv - Immunology 2023

  Quote: ... Brilliant Violet 650™ anti-mouse/human CD44 Antibody (Biolegend, Cat. 103049).
• Engineering an anti-CD206-synNotch receptor: insights into the development of novel synthetic receptors

  Sofija Semeniuk, Bin-Zhi Qian, Elise Cachat, bioRxiv - Synthetic Biology 2024

  Quote: ... PE/Cyanine5 anti-mouse/human CD45R/B220 Antibody (Biolegend; Cat. No. 103209), PE/DazzleTM 594 anti-mouse CD3 Antibody (Biolegend ...
• Bosutinib stimulates macrophage survival, phagocytosis and intracellular killing of bacteria

  Ronni A. G. da Silva, et al., bioRxiv - Microbiology 2023

  Quote: ... in the presence of 50 ng/mL recombinant human M-CSF (Biolegend) for 7 days ...