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Citations for BioLegend :
1451 - 1500 of 5067 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Genetics 2021Quote: ... Cells were then washed twice with cold PBSx1 and the cells were incubated with live/dead Zombie Violet Dye (Biolegend, 423114) for 15 min at RT as suggested by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... cells were initially blocked with anti-mouse Fcγ III/II receptor (CD16/CD32) antibodies and dead cells excluded by staining with a fixable live/dead cell-exclusion dye (BioLegend, USA). Then ...
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bioRxiv - Immunology 2022Quote: ... Non-adherent cells were removed after 30min and adherent cells were incubated with αMHC DP-DQ-DR mAb (BioLegend, clone Tü39) or isotype control (IgG2a ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with the following conjugated cell-surface marker antibodies at 1:50 dilutions: CD326 (EpCAM) PE (Biolegend, 324206) and CD45 BV786 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... to assess viable cells and fluorophore-conjugated monoclonal antibodies to the following human cell-surface markers: anti-CD45-BV650 (Biolegend, 304044), anti-CD206-AF647 (Biolegend ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in the supernatants from B cells that had been stimulated for 48 hr were measured using the LEGENDplex™ Human B cell Panel (13-plex) (Biolegend), using the same procedure as described for the antibody measurements ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellets were harvested and resuspended in 5 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 5 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... For adoptive transfer and co-culture experiments naïve OT-II cells were magnetically enriched with MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend Cat: 480040) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Immunology 2024Quote: ... CORSET8 T cells were purified from the TCR Tg donor mice with the MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... Lymph node cells were harvested from TCR Tg donor mice and CD8+ T cells were purified with MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: In order to separate cells of different origin we further stained the cell suspension with antibodies against CD45 (Biolegend #103107; 1:200), FAP (Abcam #ab28244 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: CD4+ cells were isolated from pooled splenocytes and lymph node cells with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were centrifuged at 300×g for 5 min and washed with 10 mL cell staining buffer (#420201, Biolegend, San Diego, CA). Human FLRT2 antibody (#AF2877 ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated by negative selection with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...
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bioRxiv - Cancer Biology 2021Quote: ... cell hashing was performed using TotalSeq-A antibodies (BioLegend) according to manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with DRAQ5 (Biolegend, San Diego, CA) and analyzed by an Operetta CLS high-content analysis system (PerkinElmer ...
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bioRxiv - Molecular Biology 2020Quote: ... cell-impermeant dye 7-amino-actinomycin D (7AAD, BioLegend), cells grown in 6-wells plates were collected upon trypsinization ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were incubated with Ki67-FITC antibody (Biolegend, 652410) for 30 min at room temperature followed by DAPI staining ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with Zombie Aqua viability dye (BioLegend), Fc receptor-blocking antibody (CD16/CD32 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed and then Fc blocked (BioLegend, 101320), before staining with an antibody cocktail (Extended Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... Viability of the cells was >90% (7-AAD, Biolegend), and purity for CD41a+ CD42b- was >85% and CD41a+ CD42b+ was >95% ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... The cells were stained with Annexin V APC (BioLegend) 1:50 diluted in Annexin V binding buffer (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Epithelial cell staining was performed using Epcam+ (BioLegend #118213) and CD45- (eBioscience #64-0451-82).
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with Fc Block (Biolegend) and stained with fluorochrome tagged antibodies for surface markers at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with Zombie Aqua fixable dye (BioLegend), Fc receptors were blocked using human TruStain FcX (BioLegend) ...
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bioRxiv - Systems Biology 2019Quote: ... cells were stained with CD326-AlexaFluor647 antibody (Biolegend, 118212) for 30 min at 4°C in the dark ...
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bioRxiv - Physiology 2019Quote: Cells were labeled with fluorophore-conjugated antibodies from BioLegend, including antibodies against B220 (catalog number 103206) ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were treated with TruStain FcX block (BioLegend #422301) followed by staining with BioLegend antibody panels for proliferating T cells (CD8 clone RPA-T8 #301050 ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with pre-warmed Fixation Buffer (BioLegend) at 37° for 15 min and permeabilized with pre-chilled True-Phos(tm ...
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bioRxiv - Immunology 2019Quote: ... Cell viability was assessed using Zombie Violet dye (Biolegend). Cells were suspended in 1X PBS (pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with anti-Bax antibody (6A7; Biolegend) and anti-SMAC antibody (Sigma ...
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bioRxiv - Immunology 2019Quote: ... Additional cells were surface stained with antibodies from Biolegend against CD3 (17A2) ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were removed from their plates with Accutase (BioLegend) and counted ...
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bioRxiv - Developmental Biology 2019Quote: Single cells were incubated with α-mouse FLK1 (BioLegend) and α-mouse PDGFRα (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... Cell were treated with Fc Block TruStain FcX (Biolegend) and stained in PBS + 1% BSA ...
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bioRxiv - Immunology 2021Quote: ... the cells were supplemented with Human TruStain FcX (Biolegend) to reduce non-specific antibody staining and 5uM Rhodium-103 (Fluidigm ...