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Citations for BioLegend :
1451 - 1500 of 5584 citations for IL 4 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Immunology 2019Quote: ... Some cultures were supplemented with combinations of 8µg/ml IL-34 (Generon, UK), 100 µg/ml BMP7 (RD systems, USA and 10µg/ml CSF-1 (Biolegend, USA) for the duration of the culture.
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bioRxiv - Physiology 2019Quote: ... Cell culture supernatant was collected and frozen for IL-2 analysis using LEGENDplex™ technology according to the manufacturer’s instructions (Biolegend).
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bioRxiv - Immunology 2021Quote: ... Primary bone marrow-derived murine macrophages (BMDMs) were pretreated with PBS or murine IL-27 (50 ng/ml from BioLegend) for 24 hours and then infected with S ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
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bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... cells from the dermis and epidermis were combined and cultured overnight in the presence of 10 U/ml IL-2 (Biolegend). The following antibodies were used in flow analysis of skin associated γδ T cell populations ...
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bioRxiv - Immunology 2023Quote: ... Phospho-protein stain was performed using an optimized protocol as follows: Following stain with antibodies against surface markers CD4 and IL-23R (12B2B64, Biolegend), the cells were fixed by 2% paraformaldehyde for 10 min at room temperature and washed once with cold PBS ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... or intracellular staining for APC-conjugated anti-human IL-17A (17-7179-42, eBioscience, USA) and Foxp3 (320014, BioLegend, USA) was performed according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were live-stained using PE anti-mouse CD14 antibody or APC anti-mouse TLR4 antibody (BioLegend), then fixed with 4% paraformaldehyde for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse TCRβ was labelled with the Fab fragment of anti-Mouse TCRβ clone H57-597 (Biolegend; #109201).
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bioRxiv - Cell Biology 2019Quote: ... and hashed cells from each mouse with mouse cell hashing antibodies (Totalseq™, BioLegend, San Diego, CA) before pooling and sorting ...
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bioRxiv - Immunology 2019Quote: ... Fc-Block was performed (10µg/ml purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse), after which cells were stained with Fixable Viability Staining e780 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... PE-anti-mouse MHC-I and PE-anti-mouse MHC-II (1 μL/tube) (BioLegend, California, USA) (Fig ...
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bioRxiv - Immunology 2019Quote: ... The negatively enriched cells were stained with anti-mouse PD-1 and anti-mouse KLRG1 antibodies (BioLegend), as well as ESAT-6 tetramer (as above ...
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bioRxiv - Microbiology 2021Quote: Mouse anti-HA.11 (Clone 16B12) and Mouse anti-HIS tag (Clone J099B12) were purchased from BioLegend. Rat anti-HA (Clone 3F10 ...
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bioRxiv - Immunology 2022Quote: ... Mouse anti-P2X7-PE (clone 1F11) and mouse anti-CD45-AF700 (clone 30-F11) were from Biolegend. All samples were analysed using FlowJo 10.7 in a blinded manner ...
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bioRxiv - Cell Biology 2022Quote: ... PE/Dazzle™ 594 Mouse anti-mouse CX3CR1 (Monoclonal; clone SA011F11; Biolegend; 1:300; Cat#149013, FACS). Biotin Rat anti-Mouse F4/80 (Monoclonal ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa-fluor 488 anti-mouse CD49b (Clone HMα2; 103510) and mouse anti-human LAMP2 (354303) from BioLegend, primary antibodies for mouse anti-human CD49b (611017 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells collected from each mouse were stained with Total-Seq Mouse Hashtag antibody-DNA conjugates (BioLegend) containing a unique barcode sequence according to the manufacturers’ recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Then cells were fixed with 4 % methanol-free formaldehyde in HBSS for 10 min prior to staining with either 4 μg/ml anti-ACE2 (Biolegend, clone A20069I) or anti-NRP-1 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human PVR/CD155 (Clone SKII.4, Biolegend), PE anti-human MICA (Clone 159227 ...
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bioRxiv - Immunology 2019Quote: ... anti-4-1BB PE or BV421 (4B4-1, BioLegend), anti-CD14 APC (HCD14 ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4°C in FOXP3 perm buffer (Biolegend). T-cells were analyzed with a BD LSRFortessa or FACSCanto II (BD Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: ... and CTLA-4 (PE/Cy7, clone BNI3, BioLegend #369614) on Days 18 (i.e. ...
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bioRxiv - Neuroscience 2020Quote: ... 4 μl of anti-HA antibody (Clone 16B12, BioLegend) were added to 800 μl of the cleared lysate and incubated for 4 h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 4 μL of anti-CD45-PE (clone HI30, BioLegend), and 4 μL of anti-CD193-PerCP/Cy5.5 (clone 5E8 ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1% TritonX and DAPI (4 ug/mL, #422801, Biolegend) for one hour at RT and stained with the following antibodies ...
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bioRxiv - Immunology 2019Quote: ... or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate; Biolegend). Intracellular staining was carried out using Human FoxP3 Buffer (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and fixed in 1x Fixation Buffer (4% paraformaldehyde, BioLegend). Cells were washed three times with Cell Staining Buffer and stained with Alexa Fluor 647 anti-human Ki-67 antibody (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1%TritonX and DAPI (4 ug/mL, #422801, Biolegend) for one hour at room temperature and were stained with vimentin (#677809 ...
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bioRxiv - Immunology 2023Quote: ... CD102-Alexa Fluor 647 (BioLegend; Clone 3C4 (mlC2/4)) ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4-BV421 (clone UC10-4B9; cat. #106311; BioLegend), TIM3-PECF594 (clone B8.2C12 ...
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bioRxiv - Immunology 2023Quote: ... anti-mIL-4 (11B11, BD, 557728, or BioLegend, 504119), anti-m/hIL-5 (TRFK5 ...
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bioRxiv - Plant Biology 2023Quote: ... Then 4 µg of primary antibodies (αHA antibody: Biolegend, #901502 ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD137 antibody (4-1BB; Biolegend Cat #309804) to assess CD8+ T-cell degranulation and TCR activation ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD137 antibody (4-1BB; Biolegend Cat #309804) staining.
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bioRxiv - Immunology 2021Quote: ... the cells were stained with PE anti-mouse IFNγ (clone XMG1.2) and PerCP/Cy5.5 anti-mouse TNFα (clone MP6-XT22, both from BioLegend). Isotype-matched antibodies were used to control for nonspecific binding ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Vimentin (Santa-Cruz; 2 μg/mL) and mouse anti-placental alkaline phosphatase (Biolegend; 1 μg/mL). Immunostaining for hCMV was performed as previously described [32] ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... with simultaneous depletion of mouse cells stained with anti-mouse-specific antibodies directed against CD45 and CD31 (Biolegend).
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bioRxiv - Immunology 2020Quote: For mouse lung cell staining: cell suspensions were incubated with purified anti-mouse CD16/32 (clone 93, BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... bone sections were double stained with AF647 anti-mouse F4/80 and AF488 anti-mouse CD206 (BioLegend, USA) overnight at 4°C while avoiding light.
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bioRxiv - Immunology 2023Quote: ... Cells were next resuspended in 50 µl (per mouse) lavage buffer containing 0.5 µg biotinylated anti-mouse FcεRIα (clone MAR-1; Biolegend) and incubated 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-mouse EpCAM-PE (clone G8.8, Biolegend), anti-mouse/human CD44-APC (clone !M7 ...
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bioRxiv - Cancer Biology 2021Quote: ... PerCP-conjugated anti-mouse CD86 (105025, BioLegend) and FITC-conjugated anti-mouse CD40 (124607 ...