20 Hydroxyecdysone ELISA Kit 1 Plate citations for BioLegend

Quote: ... cells in black wall 96-well-plate were washed with PBS for once and blocked with FcR blocking buffer (FcX block, Biolegend, San Diego, CA, USA) at room temperature for 10 minutes ...

CD8⁺ T-Cell Metabolic Rewiring Defined by Single-Cell RNA-Sequencing Identifies a Critical Role of ASNS Expression Dynamics in T-Cell Differentiation

Juan Fernández-García, et al., bioRxiv - Immunology 2021

Quote: ... CD8+ T cells isolated at each time point of interest were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...

CD8⁺ T-Cell Metabolic Rewiring Defined by Single-Cell RNA-Sequencing Identifies a Critical Role of ASNS Expression Dynamics in T-Cell Differentiation

Juan Fernández-García, et al., bioRxiv - Immunology 2021

Quote: ... The resulting whole-splenocyte single-cell suspensions were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...

AMPK Drives Both Glycolytic and Oxidative Metabolism in T Cells During Graft-versus-host Disease

Archana Ramgopal, et al., bioRxiv - Immunology 2023

Quote: Levels of murine cytokines in transplanted mice were assessed using the LEGENDplex™ Murine Inflammation panel (13-plex) in a V-bottom plate per manufacturer’s instructions (Cat. No: 740446, BioLegend, San Diego, CA, USA). LEGENDPlex data were acquired on a BD Fortessa analyzer (BD Biosciences ...

Neutral sphingomyelinase 1 regulates cellular fitness at the level of ER stress and cell cycle

Dolma Choezom, Julia Christina Gross, bioRxiv - Cell Biology 2022

Quote: ... FITC Annexin V Apoptosis Detection Kit with PI (BioLegend) was used according to the manufacturer’s instructions ...

Metabolic and Epigenomic Regulation of Th17/Treg Balance by the Polyamine Pathway

Chao Wang, et al., bioRxiv - Immunology 2020

Quote: ... Apoptosis was assessed using Annexin V staining kit (BioLegend). Phosphorylation of proteins to determine cell signaling was performed with BD Phosflow buffer system (BD bioscience ...

Dynamically Linking Influenza Virus Infection Kinetics, Lung Injury, Inflammation, and Disease Severity

Margaret A. Myers, et al., bioRxiv - Systems Biology 2021

Quote: ... followed by viability (Biolegend, Zombie Violet Fixable Viability Kit) and surface marker staining with anti-mouse antibodies ...

Redefining CD4 T cell residency: Helper T cells orchestrate protective humoral immunity in the lung

Nivedya Swarnalekha, et al., bioRxiv - Immunology 2020

Quote: ... Live-dead stain (Zombie Red Fixable Viability kit, Biolegend) was added to the antibody mix and stained at 4°C for 30 min ...

Tyrosine kinase inhibitors trigger lysosomal damage-associated cell lysis to activate the NLRP3 inflammasome

Emilia Neuwirt, et al., bioRxiv - Immunology 2022

Quote: ... Annexin V-FITC apoptosis staining/detection kit (Abcam, BioLegend) and acridine orange (Invitrogen) ...

Clearance of defective muscle stem cells by senolytics reduces the expression of senescence-associated secretory phenotype and restores myogenesis in myotonic dystrophy type 1

Talita C. Conte, et al., bioRxiv - Cell Biology 2022

Quote: Apoptotic cells were identified using a detection kit (Biolegend, FITC Annexin V Apoptosis Detection Kit with PI ...

Resilient anatomy and local microplasticity of naïve and stress hematopoiesis

Qingqing Wu, et al., bioRxiv - Physiology 2022

Quote: ... LEGENDScreen™ Mouse PE Kit (BioLegend, catalogue number 700005) was used according to the manufacturer’s instructions ...

CD8⁺ T cell priming in oral mucosa-draining lymph nodes supports systemic immunity

Juliana Barreto de Albuquerque, et al., bioRxiv - Immunology 2020

Quote: ... or MojoSort™ Isolation Kits (BioLegend, San Diego, US) according to the manufacturer’s protocol ...

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