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Citations for BioLegend :
1451 - 1480 of 1480 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated by negative selection with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... TF and ICS was performed following permeabilization using True-Nuclear buffer kit according to the recommended protocol (True-Nuclear Transcription Factor Buffer Set; BioLegend, San Diego, CA). Samples were acquired on a Cytek Aurora (Cytek ...
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bioRxiv - Neuroscience 2019Quote: ... Red blood cells were osmotically lysed and CD4+ T-cells were isolated using a MojoSort Mouse CD4+ T Cell Isolation Kit (Biolegend, San Diego, CA). Purified CD4s were labeled with Cell Proliferation Dye eFluor450 (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to the staining of 332 surface antigens using LEGENDScreen Human Cell Screening PE Kit (cat.no. 700001; Biolegend, San Diego, CA, USA). Data acquisition was performed on FACSVerse (Becton Dickinson (BD) ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130, San Diego, CA) following manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and proceeded to Annexin V-fluorescein isothiocyanate (FITC)/PI staining according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI; BioLegend, San Diego, CA, USA). Cells stained with Annexin V (both Annexin V+- and Annexin V+/PI+-cells ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... The levels of serum cytokines and chemokines were determined by a bead-based multiplex assay using the LEGENDplex mouse anti-virus response panel (13-plex) kit (#740622, Biolegend, San Diego, USA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: The metabolic activity of HDF cells cultured exposed to the films was analysed using a resazurin assay (Deep Blue Cell Viability Kit (BioLegend, San Diego, USA)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained for viability using Zombie NIR™ fixable viability kit for 15 min at room temperature (Cat # BioLegend, CA, USA), followed by fluorescently labeled high- or low-molecular weight HA (2.5mg/ml in PBS ...
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bioRxiv - Physiology 2023Quote: CD8+ cells were isolated from the spleens of four 8-week-old diabetic female NY8.3 NOD mice using a MojoSort Mouse CD8 T-cell isolation kit (Cat# 480043, BioLegend, San Diego, CA, USA) and MojoSort buffer (Cat# 480017 ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... naïve CD4 and CD8 T-cells were isolated from syngeneic cryopreserved PBMC aliquots using the MojoSort magnetic negative selection kits (BioLegend, 480041 and 480045). Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Immunology 2021Quote: ... were determined using bead based immunoassays (CBA kit, Cat. 560484, BD PharMingen, San Diego, CA, USA; and LEGENDplex, Cat. 740003, BioLegend, San Diego, CA, USA). Log-transformed data were used to construct standard curves fitted to 10 discrete points using a 4-parameter logistic model ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in PBS with 2% FBS containing anti-mouse CD16/CD32 antibody (table 1) and 1:1000 Fixable Viability Dye eFluor™ 506 (eBioscience, Cat#65-0866) or Zombie UV™ Fixable Viability Kit (BioLegend, Cat#423108). Staining with primary fluorophore-conjugated antibodies directed against cell surface markers (Additional file 4 ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then negatively-selected (CD8+CD44- purity > 90%) from the resulting single-cell splenocyte suspension using the MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit (BioLegend, San Diego, CA, USA). Two modifications were introduced relative to the manufacturer’s guidelines to increase purity ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Flow cytometry viability staining was conducted by suspending murine spleen and tumor single cell suspensions in Zombie Violet fixable viability kit (Cat # 423114, BioLegend, San Diego, CA, USA) according to manufacturer instructions for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Transduced T cells were positively selected with a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells ...
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bioRxiv - Microbiology 2023Quote: ... the FOXP3/Transcription Factor Staining Buffer kit (eBioscences – 00-5523-00) was used along with the following anti-mouse antibodies: BV605-conjugated anti-CD4 (Biolegend, RM4-5; 1/700), BV650-conjugated anti-B220 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were labelled with antibodies against cell surface antigens as described above and then prepared using a Cyto-Fast Fix/Perm kit (BioLegend, San Diego, CA, US), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: ... then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE) (BioLegend – 105808; 0.4 µg per million cells), rat anti-mouse CD34 (labelled with AF700 ...
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bioRxiv - Cancer Biology 2022Quote: ... and double knockdown cells were treated with the IC50 value of cisplatin and viability was assessed via Annexin V and propidium iodide staining by flow cytometry (Biolegend APC Annexin V Apoptosis Detection Kit).
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...