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Citations for BioLegend :
101 - 150 of 1146 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with a PE streptavidin conjugate (BioLegend). Stained PECs were detected using a BD LSR II flow cytometer (BD ...
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bioRxiv - Immunology 2019Quote: ... and IFNγ detection by ELISA per manufacturer’s instructions (BioLegend).
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bioRxiv - Biophysics 2023Quote: ... but with detection using anti-RpoB antibody (BioLegend NT63) instead of anti-RpoC.
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bioRxiv - Immunology 2024Quote: ... were targeted for detection using PE-Dazzle 594 (BioLegend). An Anti-CD16/CD32 Fc receptor blocking antibody was to eliminate non-specific receptor binding ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Immunology 2022Quote: ... IL-17A, IL-17F, IL-21, IL-22) and mouse Pro-inflammatory Chemokine Panel (CCL20, CXCL1, CCL2, CCL3, CCL4) (Biolegend) using a FACSVerse flow cytometer (BD Bioscience) ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and proceeded to Annexin V-fluorescein isothiocyanate (FITC)/PI staining according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI; BioLegend, San Diego, CA, USA). Cells stained with Annexin V (both Annexin V+- and Annexin V+/PI+-cells ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Microbiology 2023Quote: ... while the detection antibody (Biotin anti-mouse IFN-β; BioLegend) island Avidin-HRP (BioLegend ...
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection using anti-HA (Covance 16B12, BioLegend, Dedham, MA, USA) or antiactin antibodies (Cell Signaling ...
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bioRxiv - Microbiology 2020Quote: ... Signal detection was performed using TMB High Sensitivity Solution (BioLegend, # 421501) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Detection reagents were anti-mouse IgG-HRP (all BioLegend, London, UK). Optical densities were measured at 450 nm using a SpectraMAX i3 microplate reader (Molecular Devices ...
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bioRxiv - Immunology 2020Quote: ... Detection of biotin was performed with APC-Cy7-conjugated streptavidin (BioLegend).
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bioRxiv - Bioengineering 2020Quote: All reagents for detection of IL-2 were purchased from Biolegend. Supernatants were collected after 16 hours of coculture of 5MCAR 58α−β− cells and M12 target cells ...
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bioRxiv - Cancer Biology 2020Quote: ... BrdU-FITC antibody staining for 20 min at RT (BioLegend #364104) and 20 min incubation with PI solution (100 µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and double knockdown cells were treated with the IC50 value of cisplatin and viability was assessed via Annexin V and propidium iodide staining by flow cytometry (Biolegend APC Annexin V Apoptosis Detection Kit).
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bioRxiv - Neuroscience 2020Quote: ... followed by Avidin-HPR and detection with a TMB Substrate Set (BioLegend). Plates were washed 4 times with PBS containing 0.05% Tween-20 between each step.
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bioRxiv - Immunology 2021Quote: ... neutrophils were stained with an anti-CD66b Pacific Blue detection antibody (Biolegend). For detection of complement deposition ...
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bioRxiv - Immunology 2021Quote: ... neutrophils were stained with an anti-CD66b Pacific Blue detection antibody (Biolegend) prior to flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... and stained for 15 min at RT with annexin V (Biolegend, 640919).
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bioRxiv - Cancer Biology 2021Quote: Vcam1 detection: APC anti-mouse CD106 (Vcam1) antibody (BioLegend, San Diego, CA, USA). Cell proliferation ...
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bioRxiv - Bioengineering 2020Quote: ... Detection of the immune cells infiltrates were performed using rat anti-mCD8 (Biolegend), rat anti-mCD4 (Biolegend) ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection used biotin-conjugated anti-mouse IgG (BioLegend #405303, 0.5 μg/ml), followed by HRP-Streptavidin conjugates (BioLegend #405210 ...
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bioRxiv - Biophysics 2020Quote: ... astrocytes were labelled 1 hour at RT with anti-GFAP (1:150,644702, BioLegend) for staining GFAP ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 1h at RT using IC fixation/permeabilization buffer (Biolegend) and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... at RT with either human IgG1 isotype control (Biolegend #403502, 0.47 mg/mL), mAb C7 (0.47 mg/mL) ...
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bioRxiv - Immunology 2022Quote: ... and antibodies at a 1:200 dilution (anti-IFNγ, clone B27, Biolegend; anti-IL-17A, clone N49-653, BD Bioscience; anti-IL-22, clone 2G12A41, Biolegend; anti-IL-2, clone MQ1-17H12). Cells were washed and resuspended in FACS buffer and then analyzed on either an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: (i) Cholesterol and glycosphingolipid detection: CD4-BV711 (clone RPA-T4, BioLegend Cat# 300558, RRID:AB_2564393), CD25-BV510 (clone MA251 ...
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bioRxiv - Immunology 2020Quote: ... Detection of CD56 on cell lines was performed using clone HCD56 (1:100, Biolegend). For evaluation of cell line CD56 expression ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used for detection: anti-FMRP (MAB2160, Millipore; 6B8, Biolegend), and anti-Calnexin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... A biotinylated primary antibody was used for direct detection without signal amplification (Biolegend 339809). Positive staining was confirmed by reference to the testing information provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Detection of Aβ species was achieved using the mouse monoclonal HRP-conjugated 6E10 (Biolegend) antibody at a dilution of 1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... with a secondary (anti-rat) detection antibody labeled with Alexa Fluor-647 (Biolegend, 407511) at a 1:500 dilution ...
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bioRxiv - Immunology 2023Quote: ... Capture and biotin conjugated detection anti-mouse IL-4 antibodies were purchased from BioLegend. Anti-mouse IL-13 antibody pair were purchased from Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for at least 60 min at RT (BioLegend, San Diego, CA, USA). Slides were washed 2 times in 0.5% BSA/PBS and once in PBS at RT ...
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bioRxiv - Immunology 2022Quote: ... we lysed red blood cells using RBC Lysis Buffer for 5min at RT (BioLegend). RBC Lysis Buffer was quenched with MACS buffer and washed once ...
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bioRxiv - Immunology 2023Quote: ... and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend). Intracellular staining was performed for cytokines using antibodies for IL-5 ...
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bioRxiv - Cell Biology 2023Quote: ... and DRAQ5 (1ng/mL, incubation 5 minutes at RT; BioLegend, San Diego, CA, USA). Samples were measured using a FACS Canto II (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 times and then incubated with Avidin-HRP (Biolegend, 405103) for biotinylated probes at 1:2000 dilution for 45 minutes at room temperature ...