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Citations for BioLegend :
101 - 150 of 284 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and were fixed and permeabilized using a True-Nuclear Transcription Factor Buffer Set (BioLegend). The permeabilized cells were stained with anti-KDM2A antibody ...
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bioRxiv - Immunology 2021Quote: ... and the two pHrodo loaded sets were stained with 2μL of FITC Annexin V (BioLegend), while the DQ-Ovalbumin loaded set was stained with APC Annexin V (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilisation for intracellular staining was performed with IC perm buffer or FoxP3 Buffer set (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were stained by using True-Nuclear transcription factor buffer set (Biolegend, San Diego, CA). Briefly ...
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bioRxiv - Genomics 2022Quote: ... at room temperature for 30 minutes using True-Nuclear Transcription Factor Buffer Set (Biolegend #424401) to assess GATA3 protein level ...
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bioRxiv - Immunology 2021Quote: ... T-bet and Ki67 was performed using True-Nuclear™ Transcription Factor Buffer Set (BioLegend), according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed and permeabilized using Cyto-Fast Fix/Perm Buffer Set (#426803, BioLegend, USA), prior to staining for 20 min at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... were used to detect IgG1 antibodies upon addition of TMB substrate (TMB substrate set, Biolegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... Staining of intracellular BCL2 was performed with True-Nuclear™ Transcription Factor Buffer Set (Biolegend) as per manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend) before intracellular staining of Arginase I ...
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bioRxiv - Microbiology 2023Quote: ... the cells were stained using True-Nuclear™ Transcription Factor Buffer Set (Cat: 424401; Biolegend) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... permeabilised and stained for transcription factors using the True-Nuclear Transcription Factor Buffer Set (Biolegend, #424401). Cytokines and transcription factors in the same FACS panel were stained with the FOXP3 transcription factor staining kit (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... IL-1α and IL-6 were quantified with Mouse IFN-γ ELISA MAXTM Deluxe Set (Biolegend), Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... Cells were then fixed and permeabilized using a True-Nuclear™ Transcription Factor Buffer Set (BioLegend) and stained with anti-RORγt (Q31-378 ...
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cytokine secretion in the supernatant was evaluated by ELISA MAX Deluxe Set Human TNF-α (BioLegend), ELISA MAX Deluxe Set Human IFN-γ (BioLegend) ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine staining was performed using Cyto-Fast™ Fix/Perm Buffer Set (Cat: 426803; BioLegend) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (BioLegend 424401) following the manufacturers instructions and were then stained with anti-FOXP3 APC (Invi-trogen 17-5773-82 ...
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bioRxiv - Immunology 2023Quote: ... mIL-1β was determined by ELISA MAX™ Standard Set Mouse IL-1β kit (BioLegend# 432601). Human IL-1β and IL-18 were determined with Cisbio HTRF Cisbio Human IL1β (#62HIL1BPET ...
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bioRxiv - Immunology 2023Quote: ... cell-surface stained cells were fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (BioLegend), incubated with anti-CD5L antibody for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... IL1beta ELISAs were performed using the ELISA MAX™ Deluxe Set Human IL1beta kit (437015, Biolegend) following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... The PBMCs were then fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and IL-1β were carried out using ELISA MAXTM Standard Set (Biolegend, San Diego, CA, USA). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... BrdU-FITC antibody staining for 20 min at RT (BioLegend #364104) and 20 min incubation with PI solution (100 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of Ki67 and p21 were carried out using FoxP3 Fix/Perm Buffer set (Biolegend #421403) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Intranuclear staining of Ki-67 was performed with the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed (TrueNuclear™ TF fixation and permeabilization buffer set, BioLegend, San Diego, CA, USA) at room temperature for 60 min ...
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bioRxiv - Immunology 2022Quote: ... Collected supernatants were assessed for cytokine content using ELISA MAX™ Standard Set Mouse IFN-γ (Biolegend). DCs were analyzed for the expression of CD86 and MHC class II using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with a cytokine staining buffer set (Biolegend, Cat#420801 and Cat#421002) for cytokine/chemokine staining and a Foxp3/Transcription Factor Staining Buffer Set (eBioscience/Thermo Fisher Scientific ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Immunology 2023Quote: ... After washing cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) followed by intracellular staining for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using the True-Nuclear Transcription Factor Buffer Set (BioLegend Cat. No. 424401) using the manufacturer’s protocol with overnight fixation/permeabilization.
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bioRxiv - Immunology 2024Quote: ... were stained after cell fixation and permeabilization with True-NuclearTM transcription factor buffer set per protocol (BioLegend). Data were collected on BD LSR Fortessa or Beckman CytoFLEX flow cytometer and analyzed by FlowJo (Tree Star Inc. ...
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bioRxiv - Immunology 2024Quote: ... When appropriate NK cells were fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Zombie-Green/Violet fixable viability dyes and Foxp3 buffer set were purchased from Biolegend (San Diego, CA). Lymphocytes were stained for cell surface markers ...
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bioRxiv - Immunology 2024Quote: ... and stained for 15 min at RT with annexin V (Biolegend, 640919).
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bioRxiv - Immunology 2021Quote: ... Staining for FOXP3 followed the instruction provided by True-Nuclear Transcription Factor Buffer Set kit (BioLegend, Cat#: 424401). For apoptosis analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in PBS and fixed/permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Biolegend). Surface (CD3 ...
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bioRxiv - Pathology 2021Quote: ... were coated with purified GM-CSF capturing antibody from ELISA MAX™ Deluxe Set Mouse GM-CSF (Biolegend), left overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The upper size limit detection threshold was set by fluorescent rainbow particles with mid-range intensity and size 3.0-3.4 µm (Biolegend) resuspended in HBSS ...
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and CV05) were stained with a divergent set of antibodies: CD19-PE 1:50 (clone HIB19, BioLegend, 302207), CD38-PEcy7 1:50 (clone HIT2 ...
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bioRxiv - Neuroscience 2022Quote: ... Panels requiring intracellular staining were then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (BioLegend), and then stained with intracellular antibodies for 30 minutes at room temperature in the dark before washing ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized and stained for transcription factors using the T rue-Nuclear Transcription Factor Buffer Set (Biolegend, Cat# 424401) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: 1X Fix Concentrate from the True-Nuclear™ Transcription Factor Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 424401) was added to each sample ...
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bioRxiv - Biophysics 2020Quote: ... astrocytes were labelled 1 hour at RT with anti-GFAP (1:150,644702, BioLegend) for staining GFAP ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 1h at RT using IC fixation/permeabilization buffer (Biolegend) and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... at RT with either human IgG1 isotype control (Biolegend #403502, 0.47 mg/mL), mAb C7 (0.47 mg/mL) ...