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Citations for BioLegend :
101 - 150 of 5295 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... OT-I T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (BioLegend; San Diego, CA): TCR γ/δ (clone GL3) ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membrane was next added to 5% nonfat milk in TBST containing horseradish peroxidase-conjugate goat anti-mouse IgG1 secondary antibodies (Biolegend 405306) diluted at 1:5000 and incubated on a horizontal rocker for 60 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS supplemented with 2% FBS (PBS+) and then stained with 50µl of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (BioLegend, clone HI30), 1:50 diluted anti-human CD3-BV605 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged at 1000 rpm and resuspended in flow buffer containing the primary antibodies: rat GFP (Biolegend, 338001, 1:200), guinea pig MAP2 (Synaptic Systems ...
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bioRxiv - Bioengineering 2023Quote: 50 μm streptavidin-coated nanovials were reconstituted at a five-time dilution in Washing Buffer containing 270 nM (40 μg/mL) of biotinylated anti-CD63 antibody (Biolegend, 353018). Nanovials were incubated with antibodies for 30 minutes at room temperature on a rotator and washed three times as described above ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Cell Biology 2022Quote: Whole lung single cell suspensions were prepared as above and then blocked in SB containing 1:50 TruStain FcX™ (anti-mouse CD16/32) Antibody (BioLegend, #101319) for 10 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... Individually titrated working solutions containing one of eight hashtag antibodies were added to each of the eight respective patient samples (Biolegend; Table S13) and incubated on ice for 30 minutes followed by three magnet-based washes in FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... for 5 min on ice prior to immunostaining with a surface stain cocktail containing the following antibodies: I-A/I-E Alexa Fluor 488(Biolegend, cat.107616), CD8a PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were treated with 3% bovine albumin in PBS containing 0.3% Triton X-100 and then stained with CD31 antibody conjugated with Alexa 647 (1:100 dilution, Biolegend, San Diego, CA) and EMCN antibody conjugated with PE (1:50 dilution ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (Biolegend, clone HI30), 1:100 diluted anti-human CD3-FITC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... the pellets were resuspended and incubated for 15 min on ice in 0.5 ml MEM containing CD24-APC antibody (1:500, 101814, Biolegend, San Diego, CA). After centrifugation for 5 min at 200 × g at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted nuclei were suspended in Nuclear Stain Buffer (Sucrose Buffer containing 1:4,000 Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins Antibody (BioLegend, Cat# 682204) plus 4µM ethidium homodimer (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... containing mouse TruStain FcX (BioLegend) and the mouse cell surface protein antibody panel containing 174 oligo-conjugated antibodies (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2020Quote: ... and were stained at 20 μl of FACS buffer per 1×106 cells containing the first set of following antibodies (all from Biolegend, unless specified otherwise): biotinylated hematopoietic lineage antibodies against NK1.1 (clone PK136) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... containing FITC-conjugated annexin-V (Biolegend) and propidium iodide (PI ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with a surface stain cocktail containing the following antibodies: CD4 APC (BioLegend, cat. 100412, clone GK1.5), CD8 Alexa Fluor 700 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with CR3022 or 4A8 and a surface stain cocktail containing the following antibodies: CD169 PerCP/Cy5.5 (BioLegend (San Diego, CA], cat. 142410), SIGN-R1 PE (R&D Systems [Minneapolis ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and antibodies at a 1:200 dilution (anti-IFNγ, clone B27, Biolegend; anti-IL-17A, clone N49-653, BD Bioscience; anti-IL-22, clone 2G12A41, Biolegend; anti-IL-2, clone MQ1-17H12). Cells were washed and resuspended in FACS buffer and then analyzed on either an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... cell stimulation cocktail (containing PMA-Ionomycin, Biolegend), 1μg/ml SARS-CoV-2 S peptide pool ...
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bioRxiv - Microbiology 2021Quote: ... cell stimulation cocktail (containing PMA-Ionomycin, Biolegend), or Lassa virus (LASV ...
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bioRxiv - Microbiology 2021Quote: ... with media containing soluble CD28 (Biolegend #302943) antibody and 20 ng/ml IL-2 (Biolegend #589106 ...
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bioRxiv - Immunology 2022Quote: ... cell stimulation cocktail (containing PMA-Ionomycin, Biolegend), or SARS-CoV-2 nucleocapsid peptide pool together with 5μg/ml Brefeldin A (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... re-suspended in flow cytometry staining buffer (FACS) buffer containing 1:100 dilution of target-specific antibodies (anti PD1:cat≠329905; Biolegend, and anti-PDL1: Cat≠329705; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... before and after 1 hour incubation at room temperature in 50 μL FACS buffer containing allophycocyanin (APC)-conjugated anti-FLAG monoclonal antibody (BioLegend, diluted 1:100 in FACS buffer). For internalisation and recycling experiments ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2021Quote: ... then mounted with DAPI-containing fluoromount (BioLegend, California), cover-slipped ...
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bioRxiv - Immunology 2020Quote: ... and then staining mixture (containing anti-CD3, Biolegend, anti-CD4 ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 1:500 anti-CD45-ApCCy7 (Biolegend #103116), 1:300 anti-CD3-APC (Thermo Fisher #17-0032-80) ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 10 μL of anti-FoxP3 (Biolegend; 126408) and Ki67 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... culture media containing 20ng/ml of recombinant CCL20 (Biolegend) or conditioned media (either adjacent or lesion) ...
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bioRxiv - Microbiology 2020Quote: ... media or cell stimulation cocktail (containing PMA-Ionomycin, Biolegend), together with 1μg/ml Golgi-plug (BD ...
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bioRxiv - Immunology 2020Quote: ... media or cell stimulation cocktail (containing PMA-Ionomycin, BioLegend), together with 1 μg/mL GolgiPlug (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies (BioLegend) used were as follows:
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... with cold PBS containing Fc block (0.125 g/ml Biolegend) and antibodies ...
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bioRxiv - Immunology 2019Quote: ... A mix containing CD11c (PE-Cy7, N418 1/400 Biolegend), B220 (BV510 ...