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Citations for BioLegend :
101 - 150 of 3247 citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... T cells were similarly isolated and stained with Ghost Dye and lineage/differentiation markers (without CD19) and with TIM-3 BV711 (Biolegend clone F38-2E2), LAG-3 BV421 (Biolegend clone 11C3C65) ...
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bioRxiv - Bioengineering 2023Quote: ... Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using an APC Anti-Human IgG Fc (Biolegend) at a 1:100 dilution incubated for 1 hr with the bacteria ...
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bioRxiv - Bioengineering 2022Quote: ... then stained with APC anti-human IgG Fc Antibody clone HP6017 (Biolegend). Samples were acquired on a BD FACSymphony A5 analyzer and data were analyzed using FlowJo (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Pooled barcoded samples were treated with FC-block (Human TruStain FcX, Biolegend) for 10 minutes then surface antibody stained for 30 minutes in cell staining medium (PBS with 0.5% BSA and 0.02% sodium azide) ...
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bioRxiv - Cell Biology 2019Quote: ... Fc receptors were blocked with human IgG antibody at 1:50 (Biolegend) in PBS (pH 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... Fc receptors were blocked using Human TruStain FcX (BioLegend, #422302, 1/20) diluted in cold FACS buffer (1x PBS supplemented with 0.5% BSA and 2 mM EDTA) ...
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bioRxiv - Genomics 2021Quote: ... and incubated with Fc-receptor blocking antibody (Human TruStain FcX, BioLegend 422302) in homogenization buffer for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR-NK cells were loaded with human recombinant PD-L1- Fc (Biolegend) or ErBb2-Fc fusion proteins (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:100 human TruStain FcX (Fc Receptor Blocking Solution) (BioLegend, Cat: 422301) and 1:100 TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with Fc receptor blocking antibodies (Human TruStain FcX, Biolegend) 1:20 in Cell Staining Buffer (CSB ...
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bioRxiv - Immunology 2022Quote: ... pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend, San Diego, CA). As a negative control ...
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bioRxiv - Cell Biology 2022Quote: ... The single-cell suspension was resuspended in Fc-Block (Human TruStain FcX, BioLegend) and incubated with CD31 microbeads (Miltenyi Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... washed and incubated for 10 min with human Fc TruStain FcX (Biolegend #101320). Cells were stained for surface antigens ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were incubated with Fc Receptor Blocking Solution (Human TruStain FcX, Biolegend) according to the manufacturer’s instructions for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... added with 5 µl of Human TruStain FcX™ Fc Blocking reagent (Biolegend). Cell suspensions were then incubated at 4°C for 10 mins ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with 5 µL Fc-block (Human TruStain FcX, Biolegend 422302) and 1.5 µL of S167-180 HLA class II PE-conjugated tetramer for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 50 µL FACS containing 5 µL human Fc-block (Biolegend, 422302), and blocked for 15 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... pre-incubated and blocked with 5μl of Human Fc Receptor Blocking Solution (Biolegend) for 10 mins at room temperature then stained with 5μl/106 cells fluorescent conjugated Brilliant Violet 510™ anti-human CD14 ...
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bioRxiv - Cell Biology 2023Quote: ... 50,000 cells were used and blocked with Human TruStain FcX (Fc block, BioLegend) in FACS buffer (0.5% BSA (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... or 1:1000 (v/v) Alexafluor 647 conjugated Anti-Human IgG Fc (Biolegend) (for AT118-L Fc Fusion proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc receptors were blocked using Human TruStain FcX™ blocking solution (BioLegend, 422301) for 5 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... and Human TruStain FcX Fc receptor blocking solution (no. 422302, BioLegend; 1:400) for 5 minutes in FACS buffer (PBS containing 5% FBS and 1 mM EDTA ...
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bioRxiv - Cell Biology 2024Quote: ... All samples were blocked with Human TruStain FcX (Fc Receptor Blocking Solution; Biolegend) on ice for 10 min before immunostaining with vimentin rabbit mAb conjugated with Alexa fluor 647 (clone ...
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bioRxiv - Immunology 2023Quote: ... and PE/Cy7-conjugated secondary anti-Human IgG Fc antibody (Clone: HP6017, Biolegend) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 1x105 cells were incubated with Human TruStain FcX (Fc Receptor Blocking Solution) (Biolegend) for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... washed twice HBSS containing 2% FCS and incubated with Human Trustain Fcx (Biolegend) for 20 min on ice ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Immunology 2019Quote: ... and stained with an AF647-labeled anti-human IgG Fc antibody (Biolegend, catalog 409320). Cells were then analyzed by flow cytometry on LSRFortessa analyzer (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in 1:50 dilution of human FC blocking solution (BioLegend; #422302) and incubated on ice for 10 min ...
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bioRxiv - Immunology 2021Quote: ... non-specific binding was blocked using Fc Receptor blocking solution (Human TruStain FcX, BioLegend) and cells were stained with antibodies in PBS supplemented with 2% hiFCS for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: After blockade of Fc-receptors using Human TruStain FcX™ (BioLegend, London, United Kingdom), 5*106 PBMCs were stained with fluorescently labelled antibodies in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... re-suspended and labelled with Fc block (Human TruStain FcX™; Biolegend, London, UK) for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked by adding 2.5 μL Human TruStain™ FcX (BioLegend, #101320) per 50 μL of 200 000 cells ...
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then stained with anti-human IgG Fc-APC (Biolegend, Cat#366906) on ice for 30 min and washed with PBS twice ...
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bioRxiv - Developmental Biology 2023Quote: ... Non-specific bindings were blocked using Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend) for 10 min on ice in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 5 µl of the Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend, #422302) were added to the sample and incubated for 10 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2021Quote: ... FITC-conjugated anti-Human IgG Fc antibody (clone M1310G05, BioLegend 410719, San Diego, CA, USA) was used to detect bound anti-CTLA-4 ...
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bioRxiv - Immunology 2022Quote: ... and stained with 1.25 µg/mL anti-human IgG Fc-PE/Cy7 (Clone HP6017; Biolegend) and 5 µg/mL anti-flag-FITC (clone M2 ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with PE labeled anti-human IgG Fc secondary antibody (Biolegend) at a 1:20 dilution in 50 μl staining buffer at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of PE anti-human IgG Fc antibody (clone HP6017, BioLegend, Cat. No. 409304) buffer-exchanged into 1x PBS was added to yeast ...
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bioRxiv - Immunology 2021Quote: ... and then treated with Human TruStain FcX (Fc receptor blocking solution, Cat. No. 422302, BioLegend) for 5 mins ...
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bioRxiv - Genomics 2021Quote: ... and incubated with Human TruStain FcX™ (Fc Receptor Blocking Solution) (Biolegend, Cat#422302, USA) for 15 minutes on ice ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of Human TruStain FcX™ Fc Receptor Blocking Solution (1:400, BioLegend), and fixed with 0.5 mL methanol-free paraformaldehyde 4% (Thermo Scientific™ Pierce™ ...