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Citations for BioLegend :
101 - 150 of 9381 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... CD3 antibody (FITC-conjugated, Biolegend), HSP70 (unconjugated ...
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bioRxiv - Bioengineering 2020Quote: ... The non-macrophages cell fraction was then incubated in mouse CD4+ T cell isolation biotin-antiboy cocktail (BioLegend) and sorted ...
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bioRxiv - Immunology 2023Quote: ... 15 million PBMCs were labeled with anti-human CD3-biotin (Biolegend, 1:100) in MACS buffer (PBS supplemented with 0.5% BSA and 2mM EDTA ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... The production of cytokines and cytolytic mediators was determined in OT-1 T-cells using intracellular cytokine staining as previously described93,94 to determine interferon-gamma (anti-IFN-γ PE, Biolegend, # 505807), tumor necrosis factor-alpha (anti-TNF-α FITC ...
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bioRxiv - Immunology 2022Quote: Cells were analyzed for cell-surface markers using fluorophore-conjugated antibodies (BioLegend, eBioscience). Cell surface staining was performed in FACS Buffer (2% FBS and 2 mM EDTA in PBS ...
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bioRxiv - Immunology 2023Quote: Cells were analyzed for cell surface markers using fluorophore-conjugated antibodies (BioLegend, eBioscience). Cell surface staining was performed in FACS Buffer (2% FBS and 2 mM EDTA in PBS ...
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bioRxiv - Immunology 2023Quote: ... electroporated Jurkat cells were interrogated by pMHC-multimer staining with the following surface panel: anti-CD3 APC Fire 50 (SK7, Biolegend), anti-CD8 Pacific Blue™ (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ and CD8+ T cells from PBMC were stained with the following cell surface markers in MACS buffer at room temperature for 20 mins: CD3-AF488 (clone OKT3, Biolegend), CD14-eFluor450 (clone 61D3 ...
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bioRxiv - Immunology 2022Quote: ... Purified NK cells were surface-stained with anti-NK1.1 antibodies (Biolegend) for flow cytometry analysis ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with surface antibodies and fixed/permeabilization buffers (Biolegend) for staining with anti-IFN-γ antibody ...
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bioRxiv - Immunology 2021Quote: ... Samples were then stained by cell-surface conjugated antibodies from BioLegend diluted in autoMACS buffer (Miltenyi ...
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bioRxiv - Physiology 2020Quote: ... Samples were then stained by cell-surface conjugated antibodies from BioLegend diluted in autoMACS buffer (Miltenyi ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies specific for the cell surface antigens from Biolegend were used for the flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... cells were also surface stained with antibodies against CD45 (30F11, BioLegend), CD117 (2B8 ...
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bioRxiv - Immunology 2024Quote: ... Surface marker stains for murine samples were carried out with anti-mouse CD3 (17A2, BioLegend), anti-mouse CD4 (RM4-5 ...
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bioRxiv - Immunology 2023Quote: ... Surface antibodies were purchased from BioLegend or ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... biotin anti-CD3 (7 μg/mL) Abs for 1 h at room temperature (BioLegend, clones RA3-6B2 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822] ...
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bioRxiv - Immunology 2019Quote: Polarized CD4+ effector T cells were generated by activating purified HD naïve CD4 T cells with plate-bound anti-CD3 (UCHT1) and anti-CD28 (CD28.2) (both 5μg/ml, Biolegend) in the presence of IL-2 (50U/ml) ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 105 CTV-labelled T-cells were stimulated with 5µg/ml of plate-bound anti-CD3 (300331, Biolegend) and 5µg/ml of soluble anti-CD28 (302913 ...
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bioRxiv - Cancer Biology 2023Quote: BMDMs were cocultured with naïve CD4+ T cells and differentiated into Tregs in the presence 0.5 μg/mL (anti-CD3 BioLegend), 1ng/mL TGFβ (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... the phenotype of live T-/CAR T-lymphocytes in all the experimental conditions was assessed using the following antibodies: BV510-anti CD3 (clone OKT3, BioLegend), APC-CY7-anti CD3 (clone UCHT1 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were surface-stained with BCMA-PE and CS1-APC antibodies (BioLegend) to verify antigen knockout ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as antibodies to cell surface markers (anti-45 AF700 [Biolegend, 147716] ...
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bioRxiv - Immunology 2023Quote: ... Antibodies for surface makers of myeloid cells were: Ly6G-BV421 (BioLegend, 127628), CD11c-BV605 (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... and antibodies GL7-biotin (BioLegend), anti-mouse IgD (clone ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained with a cocktail of cell surface antibodies (Biolegend, Table 2) in 0.5%BSA/PBS and analyzed on a LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... total T cells or naïve CD4+ T cells were isolated from mouse spleen and lymph nodes using MojoSort mouse CD3/CD4 naïve T cell Isolation Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cell were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Biochemistry 2023Quote: ... Naïve CD4+ T cells were then activated with anti-CD3 (Biolegend, Cat#100340, 1:500 dilution to 5μg/mL), anti-CD28 (Biolegend ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a T cell activation analysis cocktail including: anti-mouse CD3 clone 17A2 APC conjugate (BioLegend, 100235), rat anti-mouse CD4 AlexaFluor700 conjugate (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Immunology 2022Quote: ... and a human cell surface protein antibody panel containing 277 oligo-conjugated antibodies (TotalSeq-A, BioLegend) including 6 TotalSeq-A isotype controls (table S4) ...
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bioRxiv - Immunology 2022Quote: ... All surface staining antibodies were from BioLegend. Data were collected using a FACSverse flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... CD45+ cells were then labeled for surface markers using TotalSeq-C antibodies (Biolegend). All sample and antibody information can be found in Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with 0.5μg of one of eight surface hashing antibodies (Biolegend) so that their sample of origin could be identified following sequencing across two lanes as previously described (Kendal AR 2020).
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed off and antibody secreting cells were detected using IgG biotin (BioLegend) followed by streptavidin-alkalkine phosphatase (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: Flow cytometry antibodies: mouse CD3-APC (Biolegend), mouse CD4-PE (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used: anti-CD3 Bv650 (OKT3, Biolegend), anti-CD3 FITC (SK7 ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-human CD3 antibody (Biolegend, 300306) and PE streptavidin (Biolegend ...
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bioRxiv - Bioengineering 2020Quote: ... and antibodies to human CD3 (HIT3a, BioLegend), CD45 (2D1 ...
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bioRxiv - Immunology 2022Quote: ... APC-Cy7-conjugated anti-CD3 antibody (Biolegend), APC-Cy7-conjugated anti-CD14 antibody (Biolegend) ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of conjugated CD3 antibody (Biolegend) was hybridized with 1 ul of 1 uM of a reverse complementary oligo with a Cy5 modification (IDT modification /5Cy5/) ...
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bioRxiv - Immunology 2020Quote: ... specific antibodies against human CD3 (OKT3, BioLegend), CD4 (OKT4 ...