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Citations for BioLegend :
101 - 150 of 2860 citations for Recombinant Human Coagulation Factor IX His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining for transcription factor (Foxp3) was performed on using True-Nuclear Factor kit (Biolegend) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2021Quote: Recombinant mouse GCSF (BioLegend) was injected intraperitoneally (125μg/mouse ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine SCF (BioLegend), recombinant murine G-CSF (BioLegend) ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant mouse IFNβ (BioLegend), PALA (Open Chemical Repository of the Developmental Therapeutics Program NCI/NIH) ...
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bioRxiv - Immunology 2022Quote: ... permeabilised and stained for transcription factors using the True-Nuclear Transcription Factor Buffer Set (Biolegend, #424401). Cytokines and transcription factors in the same FACS panel were stained with the FOXP3 transcription factor staining kit (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... and transcription factor staining kit (Biolegend). To assess the effect of RORγt inhibition on IL-17 production the specific inhibitor SR1001 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... or by consecutive cell sorting for the expression of fluorescent proteins (mCherry-tagged or mVenus-tagged constructs) or the re-expression of TLR2 at the cell surface (Clone T2.5, Biolegend 121810) until the cell population was homogeneous ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and tetramerized using fluorescently tagged streptavidin from Biolegend. 5,6-Dimethylxanthenone-4-acetic Acid (DMXAA ...
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bioRxiv - Immunology 2021Quote: ... metal-tagged antibodies including pan-keratin (C11, Biolegend), BDCA2 (Polyclonal ...
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bioRxiv - Immunology 2022Quote: ... and APC anti-His Tag antibody (Biolegend) for 30 min each ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized and stained for transcription factors using the T rue-Nuclear Transcription Factor Buffer Set (Biolegend, Cat# 424401) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant galectin-3 (Biolegend # 599706) was then incubated either for 1-2 hrs at RT or overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine G-CSF (BioLegend), recombinant murine ICAM-1 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... Reagents: recombinant murine CXCL1 (BioLegend), recombinant murine SCF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant murine IL-4 (Biolegend) was combined with rat IgG1 mAb to IL-4 ...
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bioRxiv - Immunology 2020Quote: Recombinant murine IL-33 (Biolegend) was injected intraperitoneally to C57BL/6 mice (100 ng/mouse) ...
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bioRxiv - Immunology 2021Quote: ... Carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Immunology 2022Quote: ... recombinant mouse IL2 (Biolegend #575408) and recombinant murine GMCSF (Peprotech #315-03 ...
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bioRxiv - Immunology 2023Quote: ... and recombinant IL-17A (BioLegend). Streptavidin HRP (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... recombinant murine IFN-γ (Biolegend) or PBS was administered by IP injection at 0.25 mg/kg on days 3-8 of DSS exposure ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant BMP6 (rBMP6) (Biolegend, USA), recombinant Follistatin (rFST ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant Follistatin (rFST) (Biolegend, USA) or vehicle control were added to the desired concentration whilst still preconfluent (approximately 24 hours before confluency) ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Immunology 2020Quote: ... True Nuclear Transcription Factor Buffer set (Biolegend) was used and cells were incubated with antibodies for intracellular staining during 40min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescently-tagged anti-mouse antibodies (BioLegend, San Diego, CA) were used to label cell surface markers for flow cytometry analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were also supplemented with a physiological growth factor cocktail consisting of 0.2 ng/ml stem cell factor (SCF, catalogue no. 573902; BioLegend), granulocyte macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Bioengineering 2023Quote: ... wherein each well mixed 50 μL of X2 photobleached conjugated magnetic beads with 50 μL solutions at different concentrations of a recombinant human Interleukin-8 (IL-8) protein (#574202, BioLegend, San Diego, CA, USA). The overall well concentration levels were 0.05 ...
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bioRxiv - Immunology 2020Quote: ... APC-anti-his tag antibody (Biolegend, clone: J095G46)) ...
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bioRxiv - Biochemistry 2022Quote: ... and Purified anti-His Tag antibody (BioLegend 652502) for 1 hour in blocking buffer shaking at rt ...
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bioRxiv - Microbiology 2023Quote: ... Phycoerythrin (PE) anti-His tag antibody (BioLegend 362603) was added to the cell suspension at a dilution of 1:20 and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 ng/mL granulocyte macrophage colony stimulating factor (GM–CSF, for M1 polarization) or 50 ng/mL macrophage colony-stimulating factor (M–CSF, for M2 polarization) (BioLegend, San Diego ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401, Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The Foxp3 Transcription Factor Staining Buffer Kit (Biolegend) was used for staining GATA3 ...
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bioRxiv - Genomics 2022Quote: ... True-Nuclear Transcription factor Buffer set (Biolegend #424401) was used according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... processed using a transcription factor buffer kit (Biolegend), and subsequently stained with antibody against FOXP3 (Biolegend).
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bioRxiv - Biophysics 2019Quote: ... 10 ng/ml basic Fibroblast Growth Factor (Biolegend). All reagents were from ThermoFisher Scientific if another supplier is not stated.
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bioRxiv - Immunology 2023Quote: ... and 50 ng/mL stem cell factor (Biolegend). Bone marrow cells were transduced with retroviral supernatants supplemented with Polybrene (6 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... True-Nuclear Transcription Factor Staining Buffer Set (Biolegend); Fixable Viability Dye (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and True-Nuclear Transcription Factor Buffer Set (BioLegend) was used to fix and permeabilize cells before staining with anti-FoxP3-PE (Clone REA788 ...