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Citations for BioLegend :
101 - 150 of 6404 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... This was followed by a 10 min incubation with anti-mouse Fc block and a 30 minute cell surface staining with anti-mouse CD4-APC/Cy7 and CD8-PE/Cy7 (Both from Biolegend). CFSE labeled CD8+ CTLs were subsequently sorted on a BD FACS Aria III (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Cytokine concentrations in supernatants harvested from CAR T cell: target cell co-cultures were determined by ELISA utilizing IFNγ and IL2 Deluxe kits (Biolegend; 431806 and 430106) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... enriched NK cells were stained with surface antibody and biotin-rat-anti mouse NKp46 (29A1.4, Biolegend) as described above ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Activated CD4+ and CD8+ T cells were stimulated with a cell activation cocktail (Biolegend; CA PMA/Ionomycin with Brefeldin A) overnight and stained for IFN-γ and TNF-α after fixation and permeabilization using the eBioscience/Thermo kit (Grand Island ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101; TNFα ELISA kit, BioLegend # 430204) or using LEGENDplex™ Human CD8/NK Panel Kit (Cat# 741186) ...
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated using MojoSortTM Human CD3 T cell Isolation Kit (Biolegend). Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated using MojoSort Mouse CD4 T cell isolation kit (BioLegend). Upon isolation ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were isolated with MojoSortTM Mouse CD4 T cell isolation kit (Biolegend) from spleens of WT mice with > 90% purity ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Immunology 2023Quote: ... CD8b (Alexa Fluor 488, 1:250, clone YTS156.7.7, Biolegend, 126628), CD45.2 (eFluor 450 ...
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bioRxiv - Immunology 2023Quote: ... and anti-rat-Ig-light-chain (Biolegend #407805, used at 1:250) for anti-mouse-PD-1-stained samples ...
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bioRxiv - Immunology 2022Quote: ... Activation of CAR-MAIT and CAR CD8+ T cells were determined with CD25 staining using CD25-APC antibody (Biolegend). CAR expression was determined with Human Her2 / ErbB2 Protein ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Immunology 2023Quote: Cytokines secreted in the culture supernatant of cytokine-stimulated sip-T were measured by a LEGENDplex Human CD8/NK Panel kit (BioLegend) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with rat polyclonal T antigen antibody (1:500) followed by an anti-rat secondary (Biolegend, 1:200). For measuring sialic acid binding dependence ...
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bioRxiv - Immunology 2022Quote: ... Total CD3+ T cells or naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD3 T Cell Isolation Kit or MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Total CD3+ T cells or naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD3 T Cell Isolation Kit or MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... or MojoSortTM CD4 T cell isolation kit (BioLegend), before being purified by FACS ...
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bioRxiv - Immunology 2024Quote: ... viable T cells were enriched using the MojoSort Mouse CD3 T Cell Isolation Kit and Mouse Dead Cell Removal Kit (BioLegend, San Diego, CA).
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...
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bioRxiv - Immunology 2022Quote: ... The 1G4 murine CD8+ T cells were additionally stained with: mouse-anti-human TCR Vβ 13.1-PE (clone: H131, Biolegend, 362409). For in vivo studies ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... reactivity of TCR-transduced PBMC-derived CD8+ T-cells was evaluated by flow analysis following anti-CD107a (BioLegend, Cat #328620) and anti-CD137 antibody (4-1BB ...
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bioRxiv - Immunology 2023Quote: ... peptide-specific T cells were evaluated by FCM by staining with PE-conjugated MART-1(A27L) tetramer and anti-CD8 FITC (BioLegend) (Supplementary Table 1).
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101 ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells isolated from donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+ T cells were purified by magnetic separation using the CD4+ T cell negative isolation kit (Biolegend) and were routinely > 95% CD3+ by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ T cells isolated from donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were isolated by negative selection using MojoSort Human CD4 T Cell Isolation kit (Biolegend). Primary CD4+ T cells were maintained in complete RPMI 1640 medium supplemented with 10 IU/ml IL-2 (Center for AIDS Reagents [CFAR] ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were separated from PBMCs using a MojoSort human CD3+ T cell isolation kit (Biolegend, USA).
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched using a MojoSort™ T cell isolation kit (BioLegend, Cat no. 480006) following the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from the spleen using MojoSort Mouse CD4 T cell isolation kit (BioLegend) and sorted according to their expression of CD44 and NKG2D ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the MojoSort Human CD3 T Cell Isolation Kit (cat. no. 480021, Biolegend) and EasySep Magnet (cat ...
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Cancer Biology 2023Quote: Single-cell suspensions from tumors or CD3 T cells isolated using MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend) following the manufacture’s protocols ...
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bioRxiv - Immunology 2022Quote: ... CD8 (anti-CD8-PeCy7; BioLegend), and IFN-γ (anti-IFN-γ-APC ...
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bioRxiv - Immunology 2020Quote: ... 116212) and T cell (PE rat anti-mouse CD4, Biolegend, 557308; FITC anti-mouse CD8a, Biolegend, 100706 ...
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bioRxiv - Immunology 2022Quote: ... 5 x 105 cells from each sample were stained for combinatorial hashing using oligonucleotide-conjugated Hashtag antibodies targeting the ubiquitous cell surface antigens Beta-2-Microglobulin and CD298 (Biolegend Totalseq A Hashtag antibodies). Hashed samples were pooled and stained together with a cocktail of 73 oligonucleotide-conjugated antibodies targeting cell surface antigens ...
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bioRxiv - Immunology 2019Quote: ... Purity of enriched naïve CD8+ T cells was determined by flow cytometry staining with anti-mouse CD8α-PerCP (Clone 53-6.7; Biolegend, CA, USA), Vα2-PE (Clone B20.1) ...
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bioRxiv - Genetics 2021Quote: ... A antibody panel was used to identify and sort the CD3+CD8+ T cell population: Human TrueStain FcX (Biolegend; Cat# 422302), PE anti-human CD45 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... IL1β ELISA kit (BioLegend) was used to measure IL1β secreted into the supernatants ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4 T cells were presorted with the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend) based on provided directions ...
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...