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Citations for BioLegend :
101 - 150 of 397 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Protein expression measurements were performed using TotalSeq-A reagents from Biolegend according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
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bioRxiv - Immunology 2020Quote: ... Bound proteins were detected by staining with fluorescently conjugated antibodies (BioLegend) and measured by flow cytometry ...
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bioRxiv - Biochemistry 2021Quote: ... USA)-conjugated anti-mouse CD206 and activated protein C (APC, BioLegend, USA)-conjugated anti-mouse CD86 at room temperature for 1 h without light ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on blots were detected using anti-RpoB (Biolegend; 663905; AB_2566583), anti-RecA (Pocono Rabbit Farm & Laboratory ...
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bioRxiv - Immunology 2024Quote: ... peridinin chlorophyll protein-cyanin 5.5 (PerCP-Cy5.5)-anti-CD16 (3G8; BioLegend), Pacific Blue-anti-CD45 (HI30 ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... p38 mitogen-activated protein kinase (p38MAPK, mouse, 1:2000, Biolegend 602651), and phosphorylated-p38MAPK (phospho-p38MAPK ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
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bioRxiv - Synthetic Biology 2023Quote: ... Secreted proteins were measured using the ELISA MAX Deluxe kits (Biolegend). Absorbance was measured at 450 nm and 570 nm using a Synergy H1 plate reader (BioTek) ...
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bioRxiv - Neuroscience 2024Quote: ... and TotalSeq anti-Nuclear Pore Complex Proteins Hashtag antibody (BioLegend 682205) (0.5 mg per million nuclei ...
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bioRxiv - Cancer Biology 2024Quote: ... biotinylated proteins were stained with streptavidin-FITC (1/200, Biolegend, 405201) and phalloidin-TRITC (1/300 ...
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bioRxiv - Neuroscience 2024Quote: ... and Mouse anti-myelin basic protein (MBP, 1:1000, Biolegend, #808401) in blocking solution at 4C ...
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bioRxiv - Immunology 2023Quote: ... DNA content was analyzed by staining cells with DAPI (BioLegend).
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bioRxiv - Immunology 2021Quote: Cytokine measurements were performed using a customized bead-based multiplex (13-LEGENDplex assay) from Biolegend, Inc ...
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bioRxiv - Molecular Biology 2021Quote: Annexin V and DAPI staining assays were performed using the apoptosis detection Kit (BioLegend, 640930) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... TNF-α and IL-17α content were determined by multiplex cytokine assays (Biolegend LEGEND plex). T cells were stimulated with anti-CD3/CD28 for 4-6 hours in the presence of brefeldin A (10μM ...
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bioRxiv - Immunology 2022Quote: ... we used the a multianalyte assay (LEGENDplex Mouse Inflammation Panel; #740446, BioLegend, San Diego, CA) according to the provided protocol.
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bioRxiv - Neuroscience 2022Quote: Enzyme-linked immunosorbent assays were performed using the mouse IL-6 ELISA-MAX kit (Biolegend), using manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Other cytokines were assayed using the LEGENDPlex multiplex assay (Human Anti-Virus Response Panel, BioLegend). Fluorescence was analyzed using a FACS Canto II (BD Biosciences).
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bioRxiv - Cell Biology 2021Quote: ... were taken on day 1 and 3 and analyzed for TGFβ using ELISA assay (BioLegend) and IFNγ and TNFα using a multiplex-bead based assay (Meso Scale Discovery ...
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bioRxiv - Immunology 2022Quote: ... mucus scrapes and culture supernatants was carried out using commercially available LEGENDplex Multiplex Assays (BioLegend), murine serum auto-antibodies were quantified using the respective commercially available Alpha Diagnostics Intl ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... ELISA assay to measure VEGFA levels was performed according to the manufacturers’ protocol (Biolegend, 430204) using the lung cancer cell supernatant.
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bioRxiv - Immunology 2023Quote: ... Supernatant was analyzed using the LEGENDPlex™ Assay (BioLegend; Human Inflammation Panel 1 (13-plex)) as per manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...
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bioRxiv - Immunology 2023Quote: A live/dead stain was included in most assays to measure viability (Zombie Yellow, BioLegend). After murine NK cell enrichment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFNɣ by ELISA (BioLegend).
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bioRxiv - Bioengineering 2023Quote: ... and IL-4 and IL-6 using a LEGENDplex™ mouse inflammation cytokine assay (Biolegend). Collected blood was incubated at room temperature for 1 hr and centrifuged for 7 min at 12,000 g at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatants collected were diluted 1:1000 prior to the IFNψ ELISA assay (BioLegend, 430104). The same co-culture procedure was performed in parallel with wildtype CD8 T cells from the same donor as a control.
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bioRxiv - Neuroscience 2024Quote: ... Cells were subjected to a JC-10 mitochondrial membrane potential assay (Cat. no. 421902, BioLegend) to assess metabolic responses to plasma and plasma-derived extracellular vesicles (pdEVs) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Biochemistry 2022Quote: ... or 0.5ng/mL of Wnt3a recombinant protein (BioLegend, San Diego, CA, #772301). Luciferase activity was measured by bioluminescence (Promega Dual-Glo Luciferase Assay System ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Bioengineering 2024Quote: The scFc protein was labeled with SNAP-Surface 488 (BG-488, Biolegend). In brief ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Genomics 2022Quote: ... Proteins of interest were visualized using an anti-Flag-PE (BioLegend, 637310), an anti-HA-Alexa647 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... WT biotinylated RBD protein conjugated with TotalSeq™-C0973 Streptavidin (BioLegend, 405275) and TotalSeq™-C0974 Streptavidin (BioLegend ...
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bioRxiv - Molecular Biology 2021Quote: ... using a LEGEND MAX™ Human IL-6 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BioLegend) with pre-coated plates ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Immunology 2021Quote: Cytokines were measured in plasma samples using the LEGENDplex™ human inflammation assay (cat. # 740809, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFN◻ by ELISA (BioLegend). All assays were performed in triplicate and average IFN◻ production was plotted with standard deviation.
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bioRxiv - Immunology 2021Quote: ... Cytokine levels were analyzed by LegendPlex TGF-β 1 and multiplex human inflammation panel assay (Biolegend). All nine parameters were detectable in every sputum sample derived from patients with AR and AA and compared to healthy control subjects.
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bioRxiv - Immunology 2022Quote: An aliquot (1×106) of total PECs were stained using a viability assay (Zombie UV, Biolegend) and blocked with 5 μg/mL anti-CD16/32 (clone 93 ...
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bioRxiv - Cancer Biology 2024Quote: The apoptosis assay was conducted using Annexin V-Pacific Blue (640918; Biolegend, San Diego, CA, USA) and 7AAD (13-6993-T500 ...
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bioRxiv - Immunology 2022Quote: Cytokine concentration in mouse plasma samples were quantified by a 3-plex LEGEND-plex assay (Biolegend) that contained capture beads and detection antibodies for CCL2 (monocyte chemoattractant protein-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cytometric bead array (CBA) assay was performed using BioLegend LEGENDplex Mouse Inflammation Panel (BioLegend, catalog #740446) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Physiology 2023Quote: ... and cytokine measurements were performed using a customized bead-based multiplex (13-LEGENDplex assay) from Biolegend, Inc ...