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Citations for BioLegend :
101 - 150 of 1221 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and cytokines were measured using a LEGENDplex™ mouse Th cytokine assay (Biolegend).
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bioRxiv - Bioengineering 2023Quote: ... and cytokines were measured using a LEGENDplex™ mouse Th cytokine assay (BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and used for cytokine ELISA assays per manufacturer’s instructions (Biolegend). Total RNAs were prepared from collected cells using Trizol (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Serum samples were analyzed using a bead-based multiplex assay (BioLegend, LEGENDplex, 740809) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and cytokines were measured using a LEGENDplex™ mouse Th cytokine assay (Biolegend).
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and used for cytokine ELISA assays per manufacturer’s instructions (Biolegend). Total RNAs were prepared from collected cells using Trizol (Invitrogen ...
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Lipid Nanoparticles from L. meyenii Walp Mitigate Sepsis through Multimodal Protein Corona FormationbioRxiv - Bioengineering 2024Quote: ... and IFN-γ by enzyme-linked immunosorbent assay (ELISA) (BioLegend, San Diego, CA). We also assessed the ability of MDNP to modulate cytokine levels in the absence of cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Blocked membranes were immunoblotted with antibodies against the following targets separately: AURKA (CST #14475, Biolegend #603301), Phospho-PKA Substrate (CST#9624) ...
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bioRxiv - Neuroscience 2020Quote: ... The top portion of the membrane was probed with 1:1000 anti-FMRP antibody (Biolegend 834601), the middle portion with 1:1000 anti-γ-Tubulin (Sigma T5326) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membranes were incubated with the Anti-FLAG epitope (DYKDDDDK, Biolegend, San Diego, CA, catalog# 637301) or Anti-HA epitope (ABclonal ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101; TNFα ELISA kit, BioLegend # 430204) or using LEGENDplex™ Human CD8/NK Panel Kit (Cat# 741186) ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used to probe the membrane: α-Neurofilament (1:10.000, Biolegend BLD-822601), α-Synapsin (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Coculture supernatants were measured using the LEGENDplex™ T helper assay (cat. # 741028, BioLegend).
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bioRxiv - Microbiology 2021Quote: ... Secreted TNF-α was quantified in supernatants by enzyme-linked immunosorbent assay (ELISA; BioLegend).
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bioRxiv - Immunology 2021Quote: Equal numbers of cells were stained with Zombie UV viability assay (Biolegend, London, UK). All samples were then blocked with 5 μg/mL anti CD16/32 (93 ...
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bioRxiv - Microbiology 2022Quote: ... Serum IL-17A was measured as part of a LEGENDPlex Multiplex protein assay (Biolegend) following the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... we used multianalyte assay (LEGENDplex Mouse B cell Panel; #740819, BioLegend, San Diego, CA) according to the provided protocol ...
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bioRxiv - Immunology 2024Quote: ... For LEGENDplex™ Mouse Cytokine Release Syndrome Panel (13-plex) assays (Biolegend, Cat. 741024), 25uL of supernatant was used following manufacturer’s protocols and samples were analysed by BD LSRFortessa.
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bioRxiv - Biochemistry 2023Quote: ... the LDH cytotoxicity assay was performed according to the manufacturer’s protocol (BioLegend, cat#426401). The colorimetric reading was recorded immediately at OD 450 nm on a microplate reader (Synergy H1 Hybrid Multi-Mode Reader ...
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bioRxiv - Cancer Biology 2024Quote: ... A bead-based assay (LEGENDplex Mouse Proinflammatory Chemokine Panel, #740007; BioLegend, San Diego, CA) that uses the principles of sandwich enzyme-linked immunosorbent assay to quantify soluble analytes using a flow cytometer was used to quantify the chemokine concentrations (CCL2 ...
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bioRxiv - Biochemistry 2022Quote: ... the transferred membrane was blocked with Blocking One and incubated with HRP-linked streptavidin (BioLegend, #405210, 1:10,000) in TBS-T containing 5% Blocking One for 1 h ...
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bioRxiv - Genetics 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with a 1:2,000 dilution of anti-RpoS antibody (Biolegend). For secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... membranes were then incubated with purified anti-complement C3/C3b/iC3b/C3d antibody (BioLegend #846302 clone 1H8/C3b) diluted to 1:5,000 in 5% milk in TBST rocking overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: Cytokine measurements were performed using a customized bead-based multiplex (13-LEGENDplex assay) from Biolegend, Inc ...
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bioRxiv - Immunology 2020Quote: ... TNF-α and IL-17α content were determined by multiplex cytokine assays (Biolegend LEGEND plex). T cells were stimulated with anti-CD3/CD28 for 4-6 hours in the presence of brefeldin A (10μM ...
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bioRxiv - Immunology 2022Quote: ... we used the a multianalyte assay (LEGENDplex Mouse Inflammation Panel; #740446, BioLegend, San Diego, CA) according to the provided protocol.
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bioRxiv - Cancer Biology 2020Quote: ... Other cytokines were assayed using the LEGENDPlex multiplex assay (Human Anti-Virus Response Panel, BioLegend). Fluorescence was analyzed using a FACS Canto II (BD Biosciences).
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bioRxiv - Cell Biology 2021Quote: ... were taken on day 1 and 3 and analyzed for TGFβ using ELISA assay (BioLegend) and IFNγ and TNFα using a multiplex-bead based assay (Meso Scale Discovery ...
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bioRxiv - Immunology 2022Quote: ... mucus scrapes and culture supernatants was carried out using commercially available LEGENDplex Multiplex Assays (BioLegend), murine serum auto-antibodies were quantified using the respective commercially available Alpha Diagnostics Intl ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... ELISA assay to measure VEGFA levels was performed according to the manufacturers’ protocol (Biolegend, 430204) using the lung cancer cell supernatant.
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bioRxiv - Immunology 2023Quote: ... Supernatant was analyzed using the LEGENDPlex™ Assay (BioLegend; Human Inflammation Panel 1 (13-plex)) as per manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: A live/dead stain was included in most assays to measure viability (Zombie Yellow, BioLegend). After murine NK cell enrichment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFNɣ by ELISA (BioLegend).
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bioRxiv - Bioengineering 2023Quote: ... and IL-4 and IL-6 using a LEGENDplex™ mouse inflammation cytokine assay (Biolegend). Collected blood was incubated at room temperature for 1 hr and centrifuged for 7 min at 12,000 g at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatants collected were diluted 1:1000 prior to the IFNψ ELISA assay (BioLegend, 430104). The same co-culture procedure was performed in parallel with wildtype CD8 T cells from the same donor as a control.
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration in the supernatant was measured by Bradford assay (BioLegend, San Diego, CA, USA). Proteins (10-40 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability assay was performed by staining cells with Zombie NIR fixable viability dye (BioLegend). Cells for immune cell profiling were stained with antibodies using BD Brilliant staining buffer and analyzed on either a five-laser Cytek Aurora spectral cytometer or a three-laser LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... c-Kit (BioLegend), EpCam (G8.8 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Biophysics 2020Quote: ... The membrane was probed with an anti-Aβ antibody targeted to residues 1-16 (1:1000, #E 610, Biolegend) and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with primary antibodies for 1h at RT with shaking (1:2000 anti-filaggrin clone Poly19058, Biolegend; 1:2000 anti-β-actin ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101 ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Immunology 2021Quote: Cytokines were measured in plasma samples using the LEGENDplex™ human inflammation assay (cat. # 740809, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Samples were normalized to 0.5 mg/mL protein in Legendplex assay buffer (Biolegend, San Diego, CA) using a Bradford Assay (BioRad ...
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bioRxiv - Synthetic Biology 2020Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFN◻ by ELISA (BioLegend). All assays were performed in triplicate and average IFN◻ production was plotted with standard deviation.
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bioRxiv - Immunology 2021Quote: ... Cytokine levels were analyzed by LegendPlex TGF-β 1 and multiplex human inflammation panel assay (Biolegend). All nine parameters were detectable in every sputum sample derived from patients with AR and AA and compared to healthy control subjects.
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bioRxiv - Immunology 2022Quote: An aliquot (1×106) of total PECs were stained using a viability assay (Zombie UV, Biolegend) and blocked with 5 μg/mL anti-CD16/32 (clone 93 ...