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Citations for BioLegend :
101 - 150 of 3514 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Molecular Biology 2022Quote: ... and resuspended in Annexin V Binding Buffer (BioLegend) at a concentration of 0.25-1.0 x 10^7 cells/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... and resuspended in Annexin V binding buffer (BioLegend). Cells were then stained with FITC Annexin V and PI for 30 minutes and the percentage of apoptotic and viable cells was analyzed by flow cytometry (BD LSRII).
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bioRxiv - Bioengineering 2020Quote: ... 500 μL of Annexin V binding buffer (Biolegend), and 500 μL of DMEM ...
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bioRxiv - Immunology 2023Quote: ... and Propidium Iodide in AnnexinV binding buffer (BioLegend) for 15 minutes at room temperature.
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bioRxiv - Plant Biology 2024Quote: ... or anti-HA (Biolegend, #901503, for INO80 binding) antibodies together with Dynabeads were added to the sonicated chromatin followed by incubation overnight to precipitate protein bound DNA fragments ...
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Immunology 2021Quote: ... Samples were thawed and proteins quantified using a LEGENDplex Human Proinflammatory Chemokine Panel immunoassay (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... APC-anti-his tag antibody (Biolegend, clone: J095G46)) ...
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bioRxiv - Biochemistry 2022Quote: ... and Purified anti-His Tag antibody (BioLegend 652502) for 1 hour in blocking buffer shaking at rt ...
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bioRxiv - Microbiology 2023Quote: ... Phycoerythrin (PE) anti-His tag antibody (BioLegend 362603) was added to the cell suspension at a dilution of 1:20 and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Immunology 2020Quote: ... was performed in Annexin V binding buffer (422201, BioLegend). Flow cytometric analysis was performed on an LSRFortessa X50 (BD Biosciences ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... 1:50 diluted in Annexin V binding buffer (BioLegend). Apoptotic cells were gated on the LIVE/DEAD-negative ...
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bioRxiv - Cell Biology 2022Quote: ... non-specific binding was blocked using TruStain FcX (Biolegend). For intracellular detection of R-spondin 2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... washed once in Annexin V binding buffer (Biolegend; 422201), resuspended in Annexin V binding buffer with Annexin V BV421 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis was quantified by annexin V-FITC binding (Biolegend). The proliferation index was determined using the Proliferation module of FlowJo (BD).
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bioRxiv - Cancer Biology 2024Quote: ... in Annexin V Binding Buffer (BioLegend, Cat. no. 422201) for 15 min at room temperature) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were detected by immunoblotting using mouse anti-human CD9 (clone HI9a, BioLegend, dilution 1:1.000), mouse anti-human CD63 (clone TS63 ...
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bioRxiv - Immunology 2022Quote: ... and a human cell surface protein antibody panel containing 277 oligo-conjugated antibodies (TotalSeq-A, BioLegend) including 6 TotalSeq-A isotype controls (table S4) ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed twice with 2% FACS buffer (1X PBS and 2% BSA) centrifuged and stained with human TruStain FcX (Biolegend, San Diego, CA) for 20 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Two microliters of mouse anti-hexa His antibody (BioLegend) were added to the 10 mL sample and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 100μl of HRP anti-His Tag Antibody (BioLegend, USA) (diluted 1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a HRP anti-His tag antibody (Biolegend, #652504) diluted 1:1000 in Superblock (Thermo #37515 ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated anti-His tag secondary antibody (BioLegend, 652503) diluted 1:2000 in blocking buffer was then added to the plates and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG ...
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bioRxiv - Bioengineering 2024Quote: ... we used HRP anti-His tag antibody (BioLegend 652503).
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-human BST-2 (clone RS38E, PE-Cy7-conjugated; Biolegend, San Diego, CA, USA) were also used as primary antibodies for cell-surface staining ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... infected or stimulated cells using a LEGENDplex Human Type 1/2/3 Interferon Panel assay (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million PBMC of each sample were first blocked with Human TruStain FcX (BioLegend; catalog #422301), then incubated with 40ng of various anti-human hashtag antibodies carrying unique cell barcodes and 12-100ng of various CITE-seq antibodies ...
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bioRxiv - Immunology 2023Quote: ... Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and anti-human Ki-67 BUV395 (1:50 ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Genomics 2020Quote: ... and incubated in 50 μl annexin-V binding buffer (Biolegend) containing FITC-conjugated annexin-V (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... followed by a wash with annexin V binding buffer (BioLegend). The cells were then stained with 0.5 µg/ml annexin V-PE (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: Binding of antibody 6H6 mIgG1 (Biolegend, PN: 306002; captured ligand) to CD123 WT ECD and variants (analytes in solution ...