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Citations for BioLegend :
101 - 150 of 3979 citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Cancer Biology 2019Quote: ... The TDEs were then washed and stained for intracellular cytokines using the PE/Cy7 anti-human IL-10 antibody (clone JES3-9D7) and Brilliant Violet 421™ anti-human LAP (TGF-β1) antibody (TW4-2F8) (BioLegend, CA). TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6 ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Immunology 2022Quote: ... cells were incubated with 40 ng/ml human IL-10 (BioLegend, San Diego, CA, USA) for 2 h at 4°C and cells were collected for flow cytometric analysis.
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Cancer Biology 2021Quote: ... CD66b (FITC, BioLegend, #305104, clone G10F5, 5 uL, human), CD33 (PE/CF594 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD14 (BV650, BioLegend, #301836, clone M5E2, 5 uL, human), ILT3 (PE/Cy7 ...
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bioRxiv - Cell Biology 2023Quote: ... and 5% CO2 (human cytokines from Biolegend or PeproTech). Cord blood CD34+ cells were additionally cultured with UM171 0.35 nM (Xcessbio) ...
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bioRxiv - Immunology 2023Quote: ... 5 µl Human TruStain FcX (Biolegend, cat. no. 422301) and 1% dextran sulfate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 anti-human CD206 and PE/Cy7 anti-human Interleukin-10 (IL-10) were used for analysis (all from BioLegend, San Diego, CA). The samples were run on an LSR Fortessa cell analyzer from BD (Becton Dickinson ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... using a LEGEND MAX™ Human IL-6 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BioLegend) with pre-coated plates ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNF-α using the LEGENDplex™ human inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... and the cell lysates were assessed on a human IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Immunology 2023Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... IL-7 and IL-23(BioLegend). CD56bright and CD56dim NK cells were expanded in NK MACS media(Miltenyi ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 and IL-7 (Biolegend) were added at 10 ng/ml final concentration ...
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bioRxiv - Immunology 2023Quote: ... IL-2 and IL-7 (Biolegend) were added at 10 ng/ml final concentration ...
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bioRxiv - Immunology 2022Quote: ... and mouse anti-human MAC1 (α-αMβ2, CBRM1/5, Biolegend) were incubated with 30 fold molar excess of sulfo-SMCC cross-linker (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... APC-human CD73 (Ecto-5’-nucleotidase) (clone AD2, 344005, Biolegend), APC/Cy7 anti-human CD90 (Thy1)(clone 5E10 ...
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bioRxiv - Cancer Biology 2021Quote: ... ILT3 (PE/Cy7, BioLegend, #333012, clone ZM4.1, 5 uL, human), Mouse IgG1 Isotype Control (PE/Cy7 ...
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bioRxiv - Immunology 2021Quote: ... Blocking Solution (5 μL of Human Trustain FcX (BioLegend #422302), and13.7 μL of a 10% Bovine Serum Albumin (BSA) ...
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bioRxiv - Immunology 2022Quote: ... Blocking Solution (5 μL of Human Trustain FcX (BioLegend #422302), and 13.7 μL of a 10% Bovine Serum Albumin (BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... and Human TruStain FcX (BioLegend, Cat#422301; 5 μl/sample) for 10 min ...
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bioRxiv - Pathology 2021Quote: ... mice were injected with 5 μg recombinant mouse IL-10 (Biolegend, San Diego, CA) or sterile saline 24 hours after APAP challenge ...
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bioRxiv - Microbiology 2020Quote: ... anti-His PE (Biolegend), and human Hashtag 3 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and cytokines (IFN-y and IL-5) were measured by ELISA (Biolegend. San Diego, CA). For intracellular cytokine determination ...
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bioRxiv - Genomics 2021Quote: After the addition of 5 ul Human TruStain FcX™ (BioLegend), the cell suspension was incubated for 10 min on ice ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Cancer Biology 2022Quote: ... at 1:5 dilution and anti-human CD45-APC (BioLegend 304012) at 1:10 dilution ...
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bioRxiv - Genomics 2023Quote: ... and blocked with 5 µL Human TruStain FcX™ (422301, Biolegend) for 10 minutes at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639, Biolegend). Antibody-tagged cells were washed three times with 3 ml of Cell Staining Buffer using centrifugation speed of 500 x g for 5 minutes at 4°C ...
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bioRxiv - Genetics 2023Quote: ... we added 5 ug/mL anti-human anti-CD3 (Biolegend-317315) and 100 ng/mL phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 1 hour with mouse HRP α-His Tag antibody (1:1000 in 5% milk) (BioLegend, USA) and developed ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... IL-6 levels of culture supernatants were determined using the LEGENDplex™ Human Inflammation Panel I (BioLegend, 740809), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Immunology 2019Quote: ... and 40ng/ml IL-18) for day 1 intracellular cytokine staining (IL-12 from R&D, IL-7 and IL-15 from Biolegend).
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bioRxiv - Immunology 2022Quote: ... IL-17A, IL-17F, IL-21, IL-22) and mouse Pro-inflammatory Chemokine Panel (CCL20, CXCL1, CCL2, CCL3, CCL4) (Biolegend) using a FACSVerse flow cytometer (BD Bioscience) ...
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bioRxiv - Immunology 2021Quote: IL-4 or IL-13 (Biolegend or PeproTech) was added into macrophage cultures at various concentrations to induce the M2/M2a phenotype in the presence or absence of a soluble CD47 extracellular domain (mCD47.ex or hCD47.ex) ...
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bioRxiv - Immunology 2020Quote: ... ELISAs for IL-1α and IL-1β (BioLegend), as well as for IFN-γ ...
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bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... IL-17A (capture Ab 5 µgml−1 TC11-18H10.1; detection Ab 2,5µgml−1 TC11-8H4, Biolegend); IFNɣ (capture Ab 5 µgml−1 AN18 ...