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Citations for BioLegend :
101 - 150 of 177 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Cell Biology 2024Quote: ... the proteins from the native gel were transferred to the PVDF membrane and probed with an anti-His-tag antibody (BioLegend). For BN-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... PDGFRa (Alexa 488 FAB1062G, R&D systems, APC 135908, Biolegend), Epcam (PE 118205 ...
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bioRxiv - Immunology 2023Quote: ... with additional anti-TCRg/d (1:50; PE; Biolegend #118108). Cells were then permeabilized using BD Fix/Perm for 20 min at RT and stained intracellularly with anti-IFN-γ (1:100 dilution ...
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: ... The media was changed with new serum free media and incubated with his tagged recombinant mouse VEGF-C (10 μg/mL) for 30 min before staining in PBS with anti-his PE (1:100, BioLegend, #362603) and cell surface markers.
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bioRxiv - Neuroscience 2024Quote: ... The cells were re-suspended in PBS / 2% HI-FBS / 10mM EDTA-Na (pH 8.0) and stained with PE/Cyanine7 anti-mouse CD45 (BioLegend, #103114, RRID:AB_312979) and FITC anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h and probed with primary antibody at 4 °C overnight with 1:500 anti-His-tag antibody (BioLegend, J099B12) and 1:200 anti-CD95L antibody (Abnova ...
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bioRxiv - Cancer Biology 2024Quote: ... As a binding control, recombinant mouse macrophage colony-stimulating factor (M-CSF) (28 kDa, with 9-His tag) (Biolegend, Cat# 576406) at 10µg/mL was immobilized using a flow rate of 20µl per minute only though channel 2 ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-aminoactinomycin D (Biolegend, Cat# 420404). After incubation for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-aminoactinomycin D (Biolegend, Cat# 420404). After incubation at 4°C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-aminoactinomycin D (Biolegend, Cat# 420404). After incubation for 20 min on ice ...
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bioRxiv - Immunology 2021Quote: ... and PE-conjugated anti-human Perforin antibody (B-D 48, Biolegend) in 0.1% PBS/0.1% saponin ...
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bioRxiv - Neuroscience 2022Quote: ... PAX6 (PRB-278P, BioLegend, 1:500; AF8150, R&D, 1:200), PHOX2B (AF4940 ...
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bioRxiv - Immunology 2024Quote: ... KIR3DL2 (clone FAB2878A; R&D) and KIR2DL5 (clone UP-R1; BioLegend). To exclude dead cells ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2021Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
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bioRxiv - Immunology 2021Quote: Immunofluorescence combining IL-33 (R&D AF3626) and CD45 (Biolegend 30-F11) antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone ...
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bioRxiv - Cell Biology 2024Quote: ... 60 ng of CD95L-IZ-His and CD95L-IZ after His-tag cleavage was loaded first on a 10% SDS-PAGE followed by a western blot probed with anti-His-tag antibody 1:1000 (BioLegend, San Diego, CA, USA). After His-tag cleavage ...
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bioRxiv - Immunology 2024Quote: ... or a control isotype-matched antibody IgG (clone Poly4053) (BioLegend, R&D systems) were added at the primary phase (day 0 and 7 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Galectin-3 (1:50; R&D systems, AF1197 & 1:100; Biolegend, 125401), anti-Hsp70 (1:300 ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... SCF (50 ng/ml) (R&D) and IL-3 (50 ng/ml) (BioLegend). For neutrophilic differentiation ...
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bioRxiv - Cancer Biology 2024Quote: TotalSeq-D Heme Oncology Panel of oligo-conjugated reagents were purchased from Biolegend (clone information can be found in Supplemental Methods) ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with Pacific Blue-Annexin V and 7AAD (7-aminoactinomycin D) (Biolegend) before running in an LSRII cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 20 μg/mL 7-AAD solution (7-amino-actinomycin D, Biolegend, 650 nm) for 15 min at room temperature in dark ...
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bioRxiv - Cell Biology 2021Quote: ... PAC-1 FITC (1:12, D Bioscience) and annexin V APC (1:60, Biolegend) for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... 1M cells were stained with TotalSeq-D Heme Oncology Cocktail (BioLegend; Cat. num. 399906) reconstituted in 59 μl of Cell Staining buffer (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptotic B16.F10 cells were stained with 7-Amino-actinomycin D (7-AAD, Biolegend) to determine viability ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-human CCR2-biotin (FAB151B; R&D Systems/ 357224; BioLegend) in FACS buffer (HBSS ...
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bioRxiv - Immunology 2021Quote: ... IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems). For data output cell number and ELISA were normalized per 1,000 of each APC subset used for co-culture.
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bioRxiv - Cancer Biology 2020Quote: ... Dead cells were excluded using 7-aminoactinomycin D (7-AAD, eBioscience) and zombie violet (BioLegend). FACS analysis was conducted using a MACSQuant analyzer (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: The PE-Annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit (BioLegend, UK) was used to detect apoptosis according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Anti-CD49f/APC (R&D, Cat.# FAB13501A) and anti-EpCAM/APC-Cy7 (Biolegend, Cat.# 118217) was used to identify Lin- stromal and epithelial subpopulations ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-CD49f/APC (R&D, Cat.# FAB13501A) and anti-EpCAM/APC-Cy7 (Biolegend, Cat.# 118217) were used to identify the lineage- (Lin- ...
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bioRxiv - Biochemistry 2022Quote: ... and 15 μg/mL of 7-amino-actinomycin D (7-AAD) (BioLegend, San Diego, CA, USA) for 2 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with 7-Amino Actinomycin D viability staining solution (7-AAD, BioLegend, San Diego, CA). The suspensions were incubated for 45 min on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... and homogenized lung were measured by ELISA according to the manufacturer’s instructions (Biolegend or R&D Systems). The levels of PGE2 were measured at the serum or the peritoneal lavage fluid of septic mice ...
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bioRxiv - Immunology 2023Quote: ... Commercial anti-RBD antibodies (mouse IgG1κ, clone 1035753, R&D systems or human IgG1κ, clone AM001414, BioLegend) and isotype controls (mouse IgG1κ ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31 ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...