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Citations for BioLegend :
101 - 150 of 3044 citations for 3 5 Diacetoxy 2 acetoxymethyl 6 phenethyl tetrahydro pyran 4 yl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed for 5 to 10 min at room temperature in 4% paraformaldehyde fixation buffer (BioLegend). Cells were permeabilized for 30 min at 4°C in PhosFlow Perm Buffer III (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 5 min at 4°C with biotinylated anti-CD19 antibody (1D3; BioLegend) to exclude CD19-positive B cells in the cell suspension using anti-biotin microbeads and LS columns ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (Biolegend, #740159) and IL-17A (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... α6 integrin (Biolegend, 313602).
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bioRxiv - Immunology 2024Quote: ... and IL-6 (Biolegend).
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bioRxiv - Immunology 2024Quote: ... mouse IL-6 (BioLegend), and mouse KC (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with 1 µM carboxyfluorescein succinimidyl ester (CFSE) dye (BioLegend, San Diego, CA, USA) for 15 minutes under standard cell culture conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Carboxy fluoroscein succinimidyl ester (CFSE) and Cell Trace Violet (CTV) were from BioLegend (San Diego, CA) and Invitrogen (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Tim-3 clone RMT 3-23 (BioLegend) and clone FAB1529 (R&D) ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cancer Biology 2024Quote: ... Residual red blood cells (RBC) were lysed incubating cell suspension for 3 minutes at 4°C with 1X RBC Lysis Buffer (BioLegend). Cell suspensions were washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with cold methanol for 3 min at room temperature and incubated overnight at 4 °C with an anti-HA antibody (diluted 1:2000) (Biolegend,). Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...
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bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Physiology 2024Quote: ... FGF23 was measured using the mouse FGF23 (c-term) ELISA (Immutopics) and IL-6 – using mouse interleukin-6 (IL-6) ELISA (BioLegend, CA, US). Serum 1,25-Dihydroxyvitamin D (1,25(OH)2D ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... CD8 BUV496 or BV650 (2:100 or 4:100; BD Biosciences Cat# 612942 or Biolegend Cat# 301042), and Live/Dead eFluor506 (5:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... they were incubated overnight at 4 °C with mouse anti-βIII tubulin (2 µg/mL, TUBB3, Biolegend). After the primary antibody incubation ...
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bioRxiv - Immunology 2023Quote: ... Trypsinized tumor cells were counted and stained with carboxyfluorescein diacetate succinimidyl ester (CFSE, 1 μL/mL; BioLegend) following the manufacturer’s protocol and cultured overnight ...
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bioRxiv - Immunology 2023Quote: ... IL-6 was measured using the ELISA MAX Standard Set Mouse IL-6 (Biolegend).
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... EBs were plated on Geltrex™-coated 6-well plates (10 EBs/well) in APEL™2 medium supplemented with VEGF (40 ng/ml) (BioLegend), SCF (50 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... and IL-6 (BioLegend, 570804). To differentiate M2 macrophages ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 6 (Biolegend, 905701), anti-pericentrin (abcam ...
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bioRxiv - Immunology 2021Quote: ... IL-6 (Biolegend, cat # 501101) and CD16/32 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... IL-6 ELISA (Biolegend, 431304); Histamine ELISA (Beckman Coulter ...
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bioRxiv - Pathology 2023Quote: ... and IL-6 (Biolegend, 431304) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... and IL-6 (BioLegend #431307) were measured by ELISA.
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bioRxiv - Immunology 2023Quote: ... anti-human IL-6 (Biolegend), or isotype control antibodies for 30 min.
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bioRxiv - Cancer Biology 2024Quote: ... IL-6(Cat. #431301, Biolegend) and glucose(Cat ...
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bioRxiv - Systems Biology 2021Quote: ... deeply anesthetized mice (5% isoflurane) were retro-orbitally injected with 3 μg of anti-CD45 antibody (PerCP, clone 30-F11, Biolegend) 3 min prior to euthanasia [46,81] ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Cell Biology 2024Quote: ... the sperm smears were fixed for 10 min with 3.2 % paraformaldehyde and permeabilized with the Intracellular Staining Perm Wash Buffer for 3 x 5 min (421002, BioLegend, USA). Following permeabilization ...
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bioRxiv - Microbiology 2021Quote: ... Sections were blocked with 10% sheep serum and 1% BSA in TBS for 30 min then incubated at 4°C overnight with 1 μg ml−1 rabbit polyclonal anti-tubulin β-3 IgG (Biolegend) and 1% BSA in TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Bioengineering 2024Quote: ... anti-mouse IgG2a/b-FITC (5 g/ml) and anti-mouse CD138-PE (4 ug/ml; BioLegend cat# 142504); ii ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...