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101 - 150 of 8186 citations for 2 Chloro 4 4 cyanophenyl 1 butene since 2020

• Enhancing and inhibitory motifs have coevolved to regulate CD4 activity

  Mark S. Lee, et al., bioRxiv - Immunology 2021

  Quote: ... were coated overnight at 4°C with 8µg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
• Proteomics reveals unique identities of human TGF-β-induced and thymus-derived CD4⁺ regulatory T cells

  Mark Mensink, et al., bioRxiv - Immunology 2022

  Quote: ... Cell surface expression of CTLA-4 was measured by adding anti-CTLA-4-PE-Dazzle594 mAb (BioLegend) in culture during restimulation for 24 h ...
• The CD4 transmembrane GGXXG and juxtamembrane (C/F)CV+C motifs mediate pMHCII-specific signaling independently of CD4-LCK interactions

  Mark S. Lee, et al., bioRxiv - Immunology 2023

  Quote: ... were coated overnight at 4°C with 8μg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
• Macrophage-Based iNos Reporter Reveals Polarization and Reprogramming in the Context of Breast Cancer

  Javier A. Mas-Rosario, et al., bioRxiv - Immunology 2022

  Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...
• Pathological changes induced by Alzheimer’s brain inoculation in amyloid-beta plaque-bearing mice

  Suzanne Lam, et al., bioRxiv - Neuroscience 2022

  Quote: ... Sections were then incubated at +4°C with the 4G8 (Biolegend 800706, 1/500), Iba1 (Wako 1919741 ...
• Native holdup (nHU) to measure binding affinities from cell extracts

  Boglarka Zambo, et al., bioRxiv - Systems Biology 2022

  Quote: ... Vimentin was performed overnight at 4°C using anti-HA (1:750, BioLegend #901502), anti-SLC1A5 (1:200 ...
• Trogocytic-molting of T-cell microvilli controls T-cell clonal expansion

  Jeong-Su Park, et al., bioRxiv - Immunology 2022

  Quote: ... Anti-LFA-1 antibody (M17/4) was purchased from BioLegend (San Diego, CA, USA). Fluorescence-conjugated CD3e ...
• Progenitor identification and SARS-CoV-2 infection in long-term human distal lung organoid cultures

  Ameen A. Salahudeen, et al., bioRxiv - Cell Biology 2020

  Quote: ... Mouse anti-TNFRSF12A (clone ITEM-4, Biolegend) was utilized for (Fig ...
• Synergistic activity of Nanog, Pou5f3, and Sox19b establishes chromatin accessibility and developmental competence in a context-dependent manner

  Liyun Miao, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 4 µg of anti-RNA polymerase II CTD repeat YSPTSPS antibody [8WG16] from Abcam (ab817) and 4 µg from BioLegend (664912) were used for each sample ...
• Antigen-derived peptides directly engage the unfolded-protein sensor IRE1α to curb cross-presentation by dendritic cells

  Ofer Guttman, et al., bioRxiv - Cell Biology 2021

  Quote: ... and 10 ng/ml IL-4 (Biolegend) for 9 days ...
• Suppression of Cytotoxic T Cell Functions and Decreased Levels of Tissue Resident Memory T cell During H5N1 infection

  Matheswaran Kandasamy, et al., bioRxiv - Microbiology 2020

  Quote: ... anti-CD4 (2µg/ml, RM4-4; Biolegend), anti-CD3 (2µg/ml ...
• High Frequencies of PD-1⁺TIM3⁺TIGIT⁺CTLA4⁺ Functionally Exhausted SARS-CoV-2-Specific CD4⁺ and CD8⁺ T Cells Associated with Severe Disease in Critically ill COVID-19 Patients

  Pierre-Gregoire Coulon, et al., bioRxiv - Immunology 2022

  Quote: ... anti-CTLA-4 (APC, clone BNI3 – BioLegend), anti-CD138 (APC-Cy-7 ...
• Heterotypic Influenza Infections Mitigate Susceptibility to Secondary Bacterial Infection

  Ellyse M. Cipolla, et al., bioRxiv - Immunology 2022

  Quote: ... Cd11a (M17/4,BioLegend, San Diego, CA), Cd127 (SB/199,Invitrogen-ThermoFisher Scientific ...
• Vaccine Elicitation of HIV Broadly Neutralizing Antibodies from Engineered B cells

  Deli Huang, et al., bioRxiv - Immunology 2020

  Quote: ... IL-4 at 50ng/ml(Biolegend, #574306), anti-CD40 at 5 μg/ml (Thermo ...
• Programming the lymph node immune response with Amphiphile-CpG induces potent cellular and humoral immunity following COVID-19 subunit vaccination in mice and non-human primates

  Lochana M. Seenappa, et al., bioRxiv - Immunology 2022

  Quote: ... IL-4 (PE, clone: 8D4-8, BioLegend), TNFα (BV605 ...
• Differential Nr4a1 and Nr4a3 expression discriminates tonic from activated TCR signalling events in vivo

  Emma Jennings, et al., bioRxiv - Immunology 2020

  Quote: ... TCRVβ5.1/5.2 APC (Clone MR9-4, BioLegend), B220 PerCPcy5.5 (Clone RA3-6B2 ...
• Acute lymph node slices are a functional model system to study immunity ex vivo

  Maura C. Belanger, et al., bioRxiv - Immunology 2020

  Quote: ... ELISA MAX detection anti-IL-4 (Biolegend), or detection TNFα (R&D Systems ...
• Antigen and Checkpoint Receptor Recalibration of T Cell Receptor Signal Strength

  Thomas A.E. Elliot, et al., bioRxiv - Immunology 2021

  Quote: ... CTLA-4 PE (clone UC10-4B9, BioLegend), OX40 APC (clone OX-86 ...
• The PD-1 checkpoint receptor maintains tolerance of self-reactive CD8 T cell in skin

  Martina Damo, et al., bioRxiv - Immunology 2021

  Quote: ... CD4 (clone GK1.5 or RM4-4, BioLegend), CD44 (clone IM7 ...
• Cellular iron governs the host response to malaria

  Sarah K. Wideman, et al., bioRxiv - Immunology 2023

  Quote: ... 10 ng/mL IL-4 (574302, BioLegend), 10 ng/mL IL-5 (581502 ...
• Dendritic cell-mediated responses to secreted Cryptosporidium effectors are required for parasite-specific CD8⁺ T cell responses

  Breanne E. Haskins, et al., bioRxiv - Immunology 2023

  Quote: ... CD11a PE (clone M17/4) from Biolegend; CD90.1 APC-ef780 (clone HIS51) ...
• A PD-1-ST2 axis controls Th2 effector function in tissue via a metabolic checkpoint

  Graham A. Heieis, et al., bioRxiv - Immunology 2023

  Quote: ... IL-4 (11B11) conjugated to BV421 (BioLegend); IL-5 (TRFK5 ...
• A PD-1-ST2 axis controls Th2 effector function in tissue via a metabolic checkpoint

  Graham A. Heieis, et al., bioRxiv - Immunology 2023

  Quote: ... 1ug/ml αIL-4 (UBC Ablab/BioLegend); or Th2 ...
• CD163 and Tim-4 identify resident intestinal macrophages across sub-tissular regions that are spatially regulated by TGF-β

  Ian E. Prise, et al., bioRxiv - Immunology 2023

  Quote: ... rat anti-Tim-4 (RMT4-54, BioLegend) or rabbit anti-CD163 (ab182422 ...
• CD163 and Tim-4 identify resident intestinal macrophages across sub-tissular regions that are spatially regulated by TGF-β

  Ian E. Prise, et al., bioRxiv - Immunology 2023

  Quote: ... and Tim-4 (RMT4-54) from BioLegend as well as CD163 (TNKUPJ ...
• Mechanical activation of TWIK-related potassium channel by nanoscopic movement and rapid second messenger signaling

  E. Nicholas Petersen, et al., bioRxiv - Neuroscience 2023

  Quote: ... 4 ug/mL apolipoprotein E3 (BioLegend, USA) was mixed with fresh 10% FBS and applied to the cells for 1hr prior to shear and or fixing.
• A Broad-Spectrum Multi-Antigen mRNA/LNP-Based Pan-Coronavirus Vaccine Induced Potent Cross-Protective Immunity Against Infection and Disease Caused by Highly Pathogenic and Heavily Spike-Mutated SARS-CoV-2 Variants of Concern in the Syrian Hamster Model

  Swayam Prakash, et al., bioRxiv - Immunology 2024

  Quote: ... anti-CTLA-4 (APC, clone BNI3 – BioLegend), anti-CD137 (APC-Cy-7 ...
• A viral-specific CD4⁺ T cell response protects female mice from Coxsackievirus B3 infection

  Aryamav Pattnaik, et al., bioRxiv - Immunology 2023

  Quote: ... CD11a (clone M17/4, BioLegend, catalog # 101106), CD62L (clone MEL-14 ...
• Suppression of autoimmune arthritis and neuroinflammation via an amino acid-conjugated butyrate prodrug with enhanced oral bioavailability

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... CTLA-4 (PE-Cy7, Cat#106314, Biolegend), CD25 (APC ...
• Analysis of regulatory network modules in hundreds of human stem cell lines reveals complex epigenetic and genetic factors contribute to pluripotency state differences between subpopulations

  Timothy D. Arthur, et al., bioRxiv - Genomics 2023

  Quote: ... and SSEA-4 (PE anti-human, Biolegend). Pluripotency was also examined using PluriTest-RNAseq 70 ...
• eQTL mapping in fetal-like pancreatic progenitor cells reveals early developmental insights into diabetes risk

  Jennifer P. Nguyen, et al., bioRxiv - Genomics 2023

  Quote: ... and SSEA-4 (PE anti-human, Biolegend) 35 ...
• Multi-omic QTL mapping in early developmental tissues reveals phenotypic and temporal complexity of regulatory variants underlying GWAS loci

  Timothy D. Arthur, et al., bioRxiv - Genomics 2024

  Quote: ... and SSEA-4 (PE anti-human, Biolegend). Pluripotency was also examined using PluriTest-RNAseq47.
• The dietary fiber guar gum ameliorates experimental autoimmune encephalomyelitis via attenuated Th1 activation and differentiation

  Naomi M Fettig, et al., bioRxiv - Immunology 2022

  Quote: ... and 4 ng/mL TGF-β (Biolegend) for mixed Th1/Th17 skewing conditions ...
• MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis

  Em P Harrington, et al., bioRxiv - Neuroscience 2022

  Quote: ... 20 ug/mL anti-IL-4 (BioLegend), 20 ug/mL anti-IFN-γ (BioLegend) ...
• Anti-TNF-induced IL-27 modulates regulatory T cell responses in patients with IBD that respond to therapy

  Raphael S. Peres, et al., bioRxiv - Immunology 2022

  Quote: ... CTLA-4 BV605 (clone UC10-4B9, BioLegend). Dead cells were excluded using efluor-780 fixable-viability dye (eBioscience) ...
• STING activation promotes autologous type I interferon-dependent development of type 1 regulatory T cells during malaria

  Yulin Wang, et al., bioRxiv - Immunology 2022

  Quote: ... and 4 μg/ml CD49b PeCy7 (BioLegend) in 30 μl PBS for 30 minutes at 37°C ...
• BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine

  Dilhan J. Perera, et al., bioRxiv - Immunology 2023

  Quote: ... IL-4-BV421 (11B11, RRID: AB_2562594, BioLegend), GrB-PE (QA1602 ...
• Increasing Signal Intensity of Fluorescent Oligo-Labeled Antibodies to Enable Combination Multiplexing

  Madeline E. McCarthy, et al., bioRxiv - Molecular Biology 2024

  Quote: ... (4) CD3 (clone UCHT1; Biolegend Cat: 300402) labeled with Atto647 int ...
• Bone marrow by day, lymph nodes by night: Undernutrition imparts a distinct circadian rhythm to T cells in mice

  Takesha R. Foster, et al., bioRxiv - Immunology 2024

  Quote: ... naïve (CD62L (MEL-4, Biolegend, California, USA) and/or CD44 (IM7 ...
• Combined PARP14 Inhibition and PD-1 Blockade Promotes Cytotoxic T Cell Quiescence and Modulates Macrophage Polarization in Relapsed Melanoma

  Rotem Leshem, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and CTLA-4 (Cat. 106338) from Biolegend. Tox (Cat ...
• SUMO Inhibition Plus CD40 Agonism Increases Anti-Tumor Immunogenicity Through Interferon Mediated Macrophage Activation

  Kevin Li, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Anti-CTLA-4 (Biolegend, Clone: UC10-4B9), Anti-CD3 (Biolegend ...
• Preclinical evaluation of a SARS-CoV-2 mRNA vaccine PTX-COVID19-B

  Jun Liu, et al., bioRxiv - Immunology 2021

  Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
• Functional single-cell profiling identifies that exosomes are associated with increased immune cell infiltration in non-metastatic breast cancer

  Mohsen Fathi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
• Developmental pathways regulate cytokine-driven effector and feedback responses in the intestinal epithelium

  Håvard T. Lindholm, et al., bioRxiv - Immunology 2020

  Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
• Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathology

  Thomas Goralski, et al., bioRxiv - Neuroscience 2023

  Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702 ...
• Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation

  Chika Mochizuki, et al., bioRxiv - Immunology 2024

  Quote: ... or 96-well plates precoated overnight at 4°C with 2 µg/ml anti-CD3ε antibody (145-2C11, BioLegend). For Th0 and Th17 differentiation ...
• Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

  Gerdien Mijnheer, et al., bioRxiv - Immunology 2022

  Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
• CSF1R inhibition by small molecule affects T-helper cell differentiation independently of microglia depletion

  Fengyang Lei, et al., bioRxiv - Immunology 2021

  Quote: ... Th1 differentiation was achieved using 1 μg/mL anti-IL-4 antibody (clone: 11B11, Biolegend), 5 ng/mL IL-2 (PeproTech) ...
• Single-cell multi-omic analysis of thymocyte development reveals drivers of CD4/CD8 lineage commitment

  Zoë Steier, et al., bioRxiv - Immunology 2023

  Quote: ... cells were incubated at 4°C overnight pERK antibody (1:20 dilution, Biolegend Cat. #675504). Samples were washed and resuspended for analysis ...