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Citations for BioLegend :
1401 - 1450 of 3192 citations for Recombinant Human ICAM1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... APC conjugated antibodies to human CD2 (Biolegend, 300214), CD4 (Biolegend ...
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bioRxiv - Genomics 2023Quote: B2M: APC anti-human B2M antibody (Biolegend, 316312)
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bioRxiv - Immunology 2024Quote: ... and phycoerythrin-conjugated anti-human CD25 (Biolegend, 356103).
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bioRxiv - Immunology 2024Quote: ... CCL4 (LegendPlex Human proinflammatory chemokines panel, Biolegend #740985). Immunoassays plates were read on a Novocyte (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... mouse anti-human CD27-PE (clone O323, BioLegend), and mouse anti-human CD38-AF647 (clone HIT2 ...
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bioRxiv - Neuroscience 2024Quote: ... and Brilliant Violet anti-human CD45 antibody (Biolegend) for gating and live / dead staining ...
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bioRxiv - Immunology 2023Quote: ... anti-human CXCR5-PE/Cy5 (clone J252D4, BioLegend), anti-human CXCR5-Alexa Fluor 647 (clone J252D4 ...
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bioRxiv - Immunology 2023Quote: ... anti-human CD27-BV711 (clone M-T271, BioLegend), anti-human CD27-PE/Cy7 (clone LG.7F9 ...
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bioRxiv - Immunology 2023Quote: ... and anti-human CD235a (clone HI264, Biolegend #349102) antibodies in HBSS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... anti-human CD8 (clone RPA-T8, Biolegend #301002), anti-human CD45 (clone HI30 ...
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bioRxiv - Microbiology 2023Quote: ... the LEGENDScreen™ Human Cell PE Kit (Biolegend) was used ...
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bioRxiv - Bioengineering 2024Quote: ... PE/Cy7 anti-human CD16 (clone 3G8, BioLegend), BV421-conjugated anti-human CD45 (clone 2D1 ...
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bioRxiv - Bioengineering 2024Quote: ... BV421-conjugated anti-human CD45 (clone 2D1; BioLegend), BV605-conjugated anti-mouse CD45 (clone 30-F11 ...
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bioRxiv - Cell Biology 2024Quote: ... PE anti-human CD117 antibody (KIT; Biolegend, 313204), or Biotin anti-human CD117 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... PE anti-human CD117 antibody (KIT; Biolegend, 313204) were used ...
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bioRxiv - Biophysics 2024Quote: ... and PE anti-human CD9 (BioLegend, cat. 312106).
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2022Quote: ... cells were pre-treated with 2.5 μg/mL anti-CD16/32 (Fc Block; 2.4G2; BioLegend, San Diego, CA) for 15 minutes before continuing with surface staining ...
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bioRxiv - Immunology 2021Quote: ... 1×105-1×106 cells were stained after Fc-blocking with TruStain FcX (BioLegend, San Diego, CA, USA) and incubation with Zombie Aqua fixable viability dye (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were aspirated and cells were then treated with Fc block (clone 93, Ref: 101302, BioLegend, 1:1000) at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with a mix of Fc Block (anti-mouse CD16/CD32 mAb; clone 2.4G2, BioLegend) and fluorophore-conjugated anti-mouse mAbs.
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Cancer Biology 2019Quote: ... Fc receptors were blocked by incubation with 2.5 μg/mL anti-CD16/32 antibody (clone 93; 101320, BioLegend) in staining buffer (25 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.02% sodium azide) and resuspended in 25 µL 1:50 mouse FC block (TruStain FcX, BioLegend Cat# 101320), and left on ice for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocked cells were initially stained with FITC-conjugated anti-CD4 and BV421-conjugated anti-CD25 (102034; BioLegend) antibodies ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 50 ng/mL anti-Fc-γ III/II (CD16/32) receptor Ab (93, BioLegend) in 100 μl of FACS Buffer (1% FBS and 1 mM EDTA in PBS) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Microbiology 2022Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... FCS files were evaluated with the LEGENDplex™ Qognit 53790 Data Analysis Suite (Biolegend, San Diego, CA, USA). A five-parameter logistic curve-fitting method was used to calculate concentrations from Median Fluorescence Intensity values ...
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
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bioRxiv - Immunology 2023Quote: ... All samples were blocked with Fc-block anti-mouse CD16/32 antibody (clone: S17011E, cat. no. 156603, BioLegend). Single-cell suspensions were stained 30min at 4°C in the dark with PerCP/Cyanine5.5 anti-mouse Ly6G (clone:1A8 ...
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bioRxiv - Immunology 2023Quote: ... and unspecific binding of antibodies to Fc receptors was blocked by incubating the cells with TruStain FcXTM (Biolegend) diluted 1:50 in FACS buffer for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Tissue samples being directly analysed ex vivo were Fc-blocked by incubation with 5μg/ml αCD16/32 (BioLegend) for 10 minutes prior to viability and surface staining ...
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bioRxiv - Immunology 2023Quote: ... All samples were blocked with Fc-block anti-mouse CD16/32 antibody (clone: S17011E, cat. no. 156603, BioLegend). After the supernatant was discarded by centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then pooled into a single tube and incubated with Tru-Stain Fc blocking solution (Biolegend) for 10 mins at RT ...
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bioRxiv - Immunology 2023Quote: ... Individual samples were then combined and stained with intracellular antibodies in CyTOF buffer containing Fc receptor blocker (BioLegend) overnight at 4C ...
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bioRxiv - Microbiology 2023Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were incubated in mouse Fc block (anti-CD16/32) antibody (Biolegend, 101335, 1:300) and then stained with the appropriate antibodies in FACS Buffer as described previously [33] ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Biochemistry 2023Quote: ... Fc-receptors were first neutralized by incubating cells on ice for 5-10 minutes with TruStain FcX (BioLegend) at 2 μg/mL in 100 μL of blocking buffer ...