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Citations for BioLegend :
1401 - 1450 of 2921 citations for Recombinant Human HFE2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and PE-labelled anti-human CD82 (Biolegend, clone ASL-24) antibodies and washed twice with 1% BSA staining solution ...
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bioRxiv - Immunology 2024Quote: ... or PE/Cyanine7 anti-mouse/human CD11b Antibody (BioLegend, #101216), and incubated in the dark for 30 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and two human-specific markers HLA-ABC-FITC (BioLegend, 311404) and hCD44-PE (BioLegend ...
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bioRxiv - Genetics 2024Quote: ... The Fc receptors were blocked with Human TruStain FcXTM (BioLegend) before staining with antibodies against CD235a and CD69 for 20 min at 4°C in the dark ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Immunology 2024Quote: ... HVEM binding to BTLA was assessed by incubating cells with the HVEM-Fc recombinant protein (20 µg/ml; Biolegend) followed by a staining with an Alexa488 conjugated-anti-IgG1 secondary Ab (clone HP6069 ...
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bioRxiv - Immunology 2024Quote: ... KO1, KO2) and treatment conditions (no IFN, IFN-β, IFN-λ) were incubated with separate oligonucleotide-tagged antibodies (HTO) for multiplexing (Biolegend TotalSeq, A0301 - A0309). Groups were counted and pooled in equal abundance ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 20 ng/ml mouse recombinant GM-CSF (Biolegend) for 8 days ...
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bioRxiv - Neuroscience 2022Quote: ... neuronal activity was recorded for 15– 30 min daily before and after treatment with recombinant CXCL11 (200 nM, BioLegend #573606) using the Maestro Pro MEA System (Axion BioSystems) ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin-streptomycin for 16 h before exposure to recombinant mouse IL-6 (rIL-6) at 50 ng/ml (575702, BioLegend) or vehicle (PBS ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 5×106 cells/mL in RPMI media with 10% FBS and 30ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF; BioLegend). After 48 hours and again after 120 hours from initial plating cell culture media was replaced with fresh media containing M-CSF ...
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bioRxiv - Bioengineering 2021Quote: ... Cytokines were determined by fluorescent bead-based multiplex sandwich immunoassays (LEGENDplex™ Human Angiogenesis Panel 1, 740697 and LEGENDplex™ Human Adipokine Panel, 740196; BioLegend, Inc., San Diego, CA) read by flow cytometry (Guava easyCyte 8HT ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend, Table S6) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... APC-conjugated anti-human IgG Fc (clone HP6017) was from Biolegend. For analysis of CD47 expression on human PBMCs ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor® 647 anti-human CD326 (EpCAM) Antibody (Biolegend 324212), PE anti-human/mouse CD49f Antibody (Biolegend 313612) ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD4 (BV711 ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD3 (APC-Cy7 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD123 BV510 (1:100, clone 6H6, BioLegend, cat # 306022), Anti-human CD34 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD69 BV650 (1:100, clone FN50, BioLegend, cat # 310934), Anti-human CD8a BV650 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TCRgd BV510 (1:100, clone B1, BioLegend, cat # 331220), Anti-human TCRab BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD56 BV605 (1:50, clone HCD56, BioLegend, cat # 318334), Anti-human CD69 BV650 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD13 BV421 (1:50, clone WM15, BioLegend, cat # 301716), Anti-human TCRgd BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD3 BV510 (1:100, clone OKT3, BioLegend, cat # 317332), Anti-human CD14 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD3 FITC (1:100, clone UCHT1, BioLegend, cat # 300440), Anti-human CD8a PerCP (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD107a BV750 (1:50, clone H4A3, BioLegend, cat # 328638), Anti-human CD103 BV711 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TCRgd FITC (1:100, clone B1, BioLegend, cat # 331208), Anti-human CD3 FITC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD326 BV650 (1:100, clone 9C4, BioLegend, cat # 324226), Anti-human CD107a BV750 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CXCR3 BV421 (1:50, clone G025H7, BioLegend, cat # 353716), Anti-human CD13 BV421 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD20 BV510 (1:100, clone 2H7, BioLegend, cat # 302340), Anti-human CD3 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CCR6 APC (1:25, clone G034E3, BioLegend, cat # 353416), Anti-human CD206 APC (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD34 BV510 (1:100, clone 581, BioLegend, cat #343528), Anti-human CD20 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD45 BV711 (1:100, clone HI30, BioLegend, cat # 304050), Anti-human CD3 BV785 (1:100 ...