Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE citations for BioLegend

Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...

Redirecting full-length FLT1 expression towards its soluble isoform promotes postischemic angiogenesis

Maja Bundalo, et al., bioRxiv - Cell Biology 2024

Quote: ... Unspecific binding to Fc receptors was blocked by incubation of cell suspension with anti-CD16/32 (Biolegend #101302, dilution 1:50) at 4°C for 10 minutes ...

In vivo PSC differentiation as a platform to identify factors for improving the engraftability of cultured muscle stem cells

Ning Xie, et al., bioRxiv - Cell Biology 2023

Quote: ... The antibodies utilized for sorting muscle stem cells (each at concentration of 0.5 μL per million cells) were PE-Cy7 anti-CD31 (BioLegend #102418 ...

Alpha-synuclein supports interferon stimulated gene expression in neurons

Aaron R. Massey, et al., bioRxiv - Neuroscience 2020

Quote: ... The following antibodies were used for intracellular flow cytometry: anti-mouse CD68 PE-Cy7 (clone FA-11, Biolegend), anti-mouse IL-17 APC ...

Nanoparticle Internalization Promotes the Survival of Primary Macrophages

Bader M. Jarai, Catherine A. Fromen, bioRxiv - Bioengineering 2021

Quote: ... and stained for intracellular markers with the following antibodies: CD206-PE-Cy7 and EGR2-APC (All from Biolegend) and analyzed using ACEA NovoCyte Flow Cytometer ...

An integrated cell atlas of the human lung in health and disease

L Sikkema, et al., bioRxiv - Cell Biology 2022

Quote: ... and the fluorophore-conjugated antibody cocktail was added in 1:1 ratio (EpCAM, Clone 9C4, PE-Cy7, BioLegend, catalog number 324222 ...

FAM21 is critical for TLR2-CLEC4E-mediated dendritic cell function against Candida albicans

Rakesh Kulkarni, et al., bioRxiv - Cell Biology 2022

Quote: ... followed by staining for 20 min on ice with a fluorescent antibody cocktail containing anti-CD11c-PE (BioLegend), anti-CD11b-APC-eflour780 (eBiosciences) ...

Naïve CD8 T cell IFNγ responses to a vacuolar antigen are regulated by an inflammasome-independent NLRP3 pathway and Toxoplasma gondii ROP5

Angel K. Kongsomboonvech, et al., bioRxiv - Immunology 2020

Quote: ... and Nlrp3-/- BMDMs were stained with anti-mouse MHC 1 Kb-PE labeled antibodies (BioLegend, clone AF6-88.5) and they were positive by FACS analysis.

REAP: A platform to identify autoantibodies that target the human exoproteome

Eric Y. Wang, et al., bioRxiv - Immunology 2021

Quote: ... 108 yeast were pelleted and resuspended in 1 mL PBE (PBS with 0.5% BSA and 0.5 mM EDTA) containing 1:100 anti-FLAG PE antibody (BioLegend). Yeast were stained at 4° for 75 minutes ...

A SARS-CoV-2 neutralizing antibody protects from lung pathology in a COVID-19 hamster model

Jakob Kreye, et al., bioRxiv - Immunology 2020

Quote: ... and CV05) were stained with a divergent set of antibodies: CD19-PE 1:50 (clone HIB19, BioLegend, 302207), CD38-PEcy7 1:50 (clone HIT2 ...

Essential role of proline synthesis and the one-carbon metabolism pathways for systemic virulence of Streptococcus pneumoniae

Elisa Ramos-Sevillano, et al., bioRxiv - Microbiology 2023

Quote: ... fluorescein isothiocyanate-conjugated polyclonal goat anti-human C3b antibody (ICN) and PE-conjugated goat anti-mouse IgG (Biolegend). For the neutrophil killing assays ...

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