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Citations for BioLegend :
1401 - 1450 of 9295 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes and ascitic cells were isolated and re-stimulated with SIINFEKL peptide (immunodominant ovalbumin peptide) for 12 hours in the presence of 2 uM Monensin (BioLegend, San Diego, CA) with the following intracellular staining of IFN-γ and measurement of IFN-γ-producing CD8+ T cells (meaning ovalbumin-specific T cells ...
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bioRxiv - Microbiology 2024Quote: ... 2 X 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 μl of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (dilution 1:200 ...
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bioRxiv - Synthetic Biology 2024Quote: ... supernatant samples were collected to measure IFN-γ and IL-2 using commercial enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions (BioLegend, 430801 and 431801).
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bioRxiv - Cell Biology 2024Quote: ... the cells were labeled for one hour with KRH buffer containing 2% FBE and the following antibodies: anti-HA (BioLegend, #901501, RRID: AB_2565006), APC-conjugated anti-LAMP1 (BioLegend ...
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bioRxiv - Developmental Biology 2021Quote: TRA-1-60 staining was performed as previously described (4) and anti-SSEA1 antibody (Biolegend, catalog no. 125604) was used to quantify MEF reprogramming ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4 °C and followed by incubation with Streptavidin conjugated to Alexa 488 (1:200, Biolegend, #405235) and an anti-mouse secondary conjugated (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated overnight at 4 °C with either α-PS1 NTF antibody (1:2000; Biolegend PS1 NT1 823401) or α-PS2 NTF antibody (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant murine IL-4 (Biolegend) was combined with rat IgG1 mAb to IL-4 ...
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bioRxiv - Microbiology 2021Quote: ... IL-4 APC (Biolegend, #504106), IL-17 PECy7 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... CD4 ([clone OKT-4] BioLegend), CD3 ([clone SP34-2] BD) ...
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bioRxiv - Immunology 2020Quote: ... anti-Vβ5 (BioLegend, MR9-4), and anti-Nur77 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... AF488-IL-4 (11B11, Biolegend), PerCP-eFluor710-IL-13 (eBio13A ...
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bioRxiv - Neuroscience 2022Quote: ... IL-4 (clone 11B11, BioLegend), IL-17a (clone eBiol7B7 ...
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bioRxiv - Immunology 2022Quote: ... CTLA-4 (UC10-4B9, Biolegend); FcR Block (120-000-826 ...
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bioRxiv - Immunology 2023Quote: ... α4-1BB-APC (17B5, Biolegend), αCD107a-APC-Cy7 (1D4B ...
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bioRxiv - Immunology 2024Quote: ... IL-4 (Biolegend cat #: 500818), and IL-17A (Biolegend cat # ...
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bioRxiv - Immunology 2024Quote: ... TIM-4 (RMT4-54, Biolegend) 1:200 ...
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bioRxiv - Immunology 2024Quote: ... IL-4 (clone: 11B11; BioLegend), IL-17A (clone ...
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bioRxiv - Immunology 2024Quote: ... CD137 (BV650, 4-1BB, Biolegend), CD8 (BV605 ...
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bioRxiv - Immunology 2021Quote: ... 1-5 * 106 cells were stained with antibodies specific for surface markers Thy1.2 (clone 30-H12, BioLegend), Thy1.1 (clone OX-7 ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and incubated with fluorescently labeled antibodies (CD4 PE, clone RM4-5, 1:400, BioLegend; CD8a FITC ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... Staining was performed by washing with 0.1% Triton-X 100 in PBS for 3 x 15 minutes then staining for CD3-PE (1:100, Biolegend, 100205) overnight at room temp ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5, BioLegend), CD3e (145-2C11 ...
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bioRxiv - Immunology 2020Quote: ... CD4 (RM4-5; BioLegend), CD8 (53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... CD4 (RM4-5, Biolegend), CD8a (53-6.7 ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... CD4 (RM4-5, Biolegend), CD80 (16-10A1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Keratin 5 (BioLegend Poly19055) (1:1,000 WB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... CD4 (RM4-5; Biolegend), CD8 (54-6.7 ...
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bioRxiv - Immunology 2022Quote: ... IL-5 (TRFK5; BioLegend), IL-4 (11B11 ...
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bioRxiv - Cell Biology 2023Quote: ... α5 integrin (Biolegend, 921704), α6 integrin (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... CD4 (RM4-5; Biolegend), NKp46 (29A1.4 ...