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Citations for BioLegend :
1401 - 1450 of 8294 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...
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bioRxiv - Molecular Biology 2024Quote: ... (2) CD8 (clone RPA-T8; Biolegend Cat: 301002) labeled with Atto488 int ...
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bioRxiv - Bioengineering 2024Quote: ... and 50 ng/mL recombinant IL-2 (BioLegend) in RPMI + 10% heat-inactivated FBS for 3 days before use.
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bioRxiv - Immunology 2024Quote: ... and CD138-PE-Cy7 (Clone: 281-2; Biolegend). The cells were washed twice ...
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bioRxiv - Immunology 2024Quote: ... BV605-labelled anti-CD25 (clone: 281-2; BioLegend), PE-Cy7-labelled anti-CD24 (clone ...
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bioRxiv - Cell Biology 2024Quote: PE anti-mouse CD63 (clone NVG-2, Biolegend)
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bioRxiv - Immunology 2024Quote: ... CD63 PE-CF594 (clone: NVG-2, 143913; BioLegend), P2X7R PE-Cy7 (clone ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 2% neutral-buffered formalin diluted in FACS buffer following addition of Zombie Yellow fixable viability dye (BioLegend #423103, 1:200 in PBS).
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 μl of FACS Buffer and incubated with 1 μl of allophycocyanin (APC)-conjugated anti-CD46 antibody (BioLegend, clone TRA-2–10, 352405) for 30 min at 4°C with rotation ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for NK cells: (donors 1-2) anti-IFN-γ FITC (BioLegend catalog #506504, clone B27), anti-CD19 PerCP/Cy5.5 (BioLegend catalog #309504 ...
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bioRxiv - Immunology 2024Quote: ... KO1, KO2) and treatment conditions (no IFN, IFN-β, IFN-λ) were incubated with separate oligonucleotide-tagged antibodies (HTO) for multiplexing (Biolegend TotalSeq, A0301 - A0309). Groups were counted and pooled in equal abundance ...
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lcn-2 levels were determined in fecal supernatants using a LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit (BioLegend #443708).
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Supernatants were analyzed using the Legendplex mouse T helper cytokine panel version 3 (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were suspended in 3 mL of RBC lysis buffer (Biolegend, CA, USA), allowed to stand at room temperature for 3 minutes ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD38 (cat. # 102705, clone 90, FITC, 3 μl, Biolegend, San Diego, CA), anti-mouse CD80 (cat ...
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bioRxiv - Cell Biology 2022Quote: ... or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416, Biolegend) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed 3 times with PBS and counter stained with DRAQ5 (BioLegend) to stain DNA for 10 min ...
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bioRxiv - Immunology 2023Quote: ... B cells were stimulated in vitro with anti-CD40 antibody (clone HM40-3, Biolegend) overnight to fully activate HEL+ B cells ...
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bioRxiv - Immunology 2019Quote: ... Plasma IL-6 levels were determined by sandwich enzyme-linked immunosorbent assay (ELISA) (Biolegend ELISA MAX Deluxe, London UK). Values below the blank were reported at the limit of detection for statistical purposes ...
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Immunology 2019Quote: TNF-α and IL-6 were measured in the media of activated BMDMs using ELISA kits purchased from Biolegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and stained with PE anti-IL-6 and PE-Cy7 anti-IL-10 (JES3-9D7) Abs (Biolegend).
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... For intracellular staining cells were either permeabilized with 0.5% Saponin or 0.1% Triton and stained for TNF-α and/or IL-6 (BioLegend). Cells were washed with 2% serum supplemented 1X PBS and subsequently fixed with 1% PFA ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... male mice were isolated 6 dpi with NP-OVA/alum and stained with anti-CD4 Alexa Fluor 647 (GK1.5, BioLegend), anti-TCRβ PE-Cy7 (H57-597 ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 3.7% formaldehyde for 6 min and PM HA-GLUT4-GFP detected by an anti-HA antibody (901503, BioLegend). HA staining was visualized by Cy3-goat anti-mouse secondary antibody (115-165-062 ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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bioRxiv - Immunology 2021Quote: ... fixed with 4% paraformaldehyde fixation buffer (BioLegend 420801, San Diego, CA, USA) for 20 minutes at room temperature and washed twice more with MACS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated at 4°C overnight with ATF4 antibody (BioLegend) at 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... for surface staining and BV605-anti-IL-4 (504125, BioLegend Europe BV), APC-anti-IL-5 (504306 ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed for 20 min with 4% paraformaldehyde (Fixation buffer; BioLegend). When intracellular staining was performed ...
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bioRxiv - Neuroscience 2021Quote: ... and CD284 (TLR-4, clone SA15-21, PE, 2.5 µg/ml Biolegend). After staining ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... and further stained with BV421-conjugated anti-mouse IL-4 (Biolegend, 504127) and ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4 °C with Zombie Violet staining (BioLegend, 423113) to exclude dead cells ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% paraformaldhyde and permeabilised with CytoPerm buffer (Biolegend). Cells were analysed on BD LSR Fortessa X-20 cytometer ...
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bioRxiv - Cancer Biology 2020Quote: ... rotating at 4°C with either μ5g IRF3 antibody (BioLegend, clone:12A4A35) or equivalent isotype control conjugated with protein A/G magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-AF647 (clone QA17A19 ...