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Citations for BioLegend :
1351 - 1400 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were stained with PE-coupled anti-Acetylated Lysine Antibody or PE-coupled Mouse IgG2b κ Isotype control Antibody (isotype control) (both Biolegend) for 30 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Genetics 2020Quote: Immunoprecipitation and immunoblotting were carried out according to standard protocols with the following antibodies: rat anti-Flag L5 antibody (BioLegend; 637301), rabbit anti-GFP antibody (Novus ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Neuroscience 2023Quote: ... non-fat dried skimmed milk powder and then probed with ICSM35 anti-PrP antibody (D-Gen Ltd; 1:5000 for RML) or with 6D11 anti-PrP antibody (BioLegend (808004); 1:5000 for ME7 ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blotting was performed using an anti-HA mouse monoclonal antibody (Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody, BioLegend) as primary antibody diluted 1:1000 in TBS-T (20 mM Tris ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: The resultant single cells were stained with cocktails of antibodies diluted with FACS buffer listed as follows: PE-conjugated anti-mouse/human CD324 (E-Cadherin) antibody (1:500, Biolegend, 147303), PE/Cy7-conjugated anti-mouse CD117 (c-Kit ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Genetics 2022Quote: ... Differentiation efficiency was measured using Fluorescence Activated Cell Sorting (FACS) following staining with an APC conjugated antibody to HLAG (APC anti-human HLA-G Antibody Clone: 87G, BioLegend UK), in unmodified hTSC this was consistently between 40-70% ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The blocking buffer was subsequently removed and cells were incubated for an additional hour with 1:100 diluted primary antibody (αHA antibody, Biolegend, #901502) in antibody dilution buffer (1x PBS ...
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bioRxiv - Bioengineering 2023Quote: Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend, 368534) and anti-IFN-γ (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for detection of MDSCs contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106), a rat anti-mouse Ly-6G/Ly-6C (Gr1 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Aspecific antibodies binding was minimized by treating the cells with TruStain FcX anti-CD32/CD16 blocking antibody (1:50, BioLegend # 422301) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Aspecific antibodies binding was minimized by treating the cells with TruStain FcX anti-CD32/CD16 blocking antibody (1:50, BioLegend # 422301) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Aspecific antibodies binding was minimized by treating the cells with TruStain FcX anti-CD32/CD16 blocking antibody (1:50, BioLegend # 422301) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and one of the hashtag antibodies (TotalSeq™-C0251 anti-human Hashtag 1 Antibody, LNH-94 2M2, BioLegend #394661; TotalSeq™-C0252 anti-human Hashtag 2 Antibody, LNH-94 2M2, BioLegend #394663 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CD16/CD32 blocking antibody (Ultra-LEAF Purified Anti-mouse CD16/32 Antibody, Biolegend, San Diego, CA, USA), to prevent phagocytosis ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Bioengineering 2024Quote: ... the beads were resuspended at a 1:10 ratio (10x the volume originally pipetted from the colloid stock bottle) in 0.2% BSA and biotinylated antibody (anti-CD63 antibody, Biolegend Cat #353017) was added at 10 µg per mL of diluted colloids and incubated on a hula rocker for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed and stained for CCNA2 (Cyclin A2) using a primary antibody (PE anti-Cyclin A Antibody – Biolegend – REF: 644004) at a 1:200 dilution in blocking solution (1% BSA in PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h and probed with primary antibody at 4 °C overnight with 1:500 anti-His-tag antibody (BioLegend, J099B12) and 1:200 anti-CD95L antibody (Abnova ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibodies were used to immunotag nuclei: anti-H2A.X-Phosphorylated (Ser139) antibody conjugated to APC (cat no. 613416, BioLegend, San Diego CA), anti-ATM-phospho (Ser1981) ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... PE rat IgG2a-isotype control antibody and PE mouse IgG2b-d κ isotype control antibody were purchased from BioLegend (Sandiego, CA, USA) and PE mouse IgG1-κ Isotype control antibody from BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: The following antibodies were used for flow cytometry: Brilliant Violet 510™ anti-mouse CD45 Antibody (BioLegend, catalog #103137, clone 30-F11), PE/Dazzle™ 594 anti-mouse CD3ε Antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2022Quote: ... mice were injected into the retro-orbital sinus with a total of 3 mg of PE/Cy7-CD45.2 (104, BioLegend) in 200 μl sterile DPBS ...
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bioRxiv - Immunology 2024Quote: ... about 3-4 million CD8+ T cells were blocked with Fc receptor blocking solution (Human TruStain FcX, BioLegend, #422302) for 10 minutes on ice and stained with BEAM-T assemblies for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... with the addition of either DCs (1 cell:3 Tresp) with 0.5 μg/ml purified anti-CD3 (145-2C11, Biolegend), or Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... cell hashing was performed using TotalSeq-A antibodies (BioLegend) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-TNF neutralizing antibody (#506325) was obtained from Biolegend.
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bioRxiv - Cell Biology 2020Quote: ... surface-stained using antibodies raised against cKIT (2B8; Biolegend) and SCA1 (D7 ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (BV421-conjugated donkey anti-rabbit (406410, Biolegend) were added in brilliant stain buffer (BD ...
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bioRxiv - Cell Biology 2020Quote: Mouse anti-beta-actin antibody (clone 2F1-1, Biolegend)
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bioRxiv - Immunology 2021Quote: ... the following antibodies were used (all purchased from BioLegend): anti- CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... The antibody cocktail included FITC-CD45 (BioLegend Cat# 103108), BV421-CD44 (BioLegend Cat# 103040) ...