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Citations for BioLegend :
1351 - 1400 of 8898 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Cell Biology 2019Quote: ... with 2□mM EDTA for flow analysis or resuspended in PBS (pH 7.2) with 2□mM EDTA and 1□μg/ml DAPI (Biolegend) for sorting ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2019Quote: ... against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ: PE-Cy7, clone XMG1.2; IL2: PE, clone JES6-5H4; TNFα: APC, clone MP6-XT22; all Biolegend. all 1:200). Next ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... single-cell spleen suspensions from C57BL/6J mice were cultured at 5 million cells/ mL in complete RPMI with 1 μg/mL anti-CD3 (BioLegend, #100340) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Immunology 2024Quote: The Fc receptors were blocked on 5X105 neutrophils resuspended in PBS with 5% FBS and TruStain FcX (1:50, BioLegend, 101320). Neutrophils were incubated with FITC conjugated anti-mouse Ly6G (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend, #406406) diluted 1:500 in 1x Permeabilisation buffer ...
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bioRxiv - Immunology 2021Quote: RAW264.7 cells of BMDMs were collected after washing in ice-cold PBS and then detaching the cells with Accutase (BioLegend). Prior to collection ...
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bioRxiv - Immunology 2021Quote: ... processing omitted the 7-AAD step and incubated with a fixable live dead dye prior to incubation with fixation buffer (Biolegend) (15 mins) ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... or biotin, 2G5, Beckman Coulter, or Ber-ACT8, Brilliant Violet 711, BUV395, BD Biosciences, or Ber-ACT8, PE-Cy-7, Biolegend), KIR2DL1 (FAB1844F ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD11b expression MDS92 and MDS-L cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD11b (BioLegend) (#557743) ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and stimulated T cells with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used (mouseCD45-FITC from BD bioscience and 7-AAD, humanCD45-PE/Cy7, humanCD19-PE, humanCD3-APC and humanCD14-APC/Cy7 from Biolegend) and cells analyzed on the LSRFortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... 50µL of blood was stained for flow cytometry using conjugated antibodies (mouse CD45-FITC from BD bioscience and 7-AAD, humanCD45-PE/Cy7, humanCD19-PE, humanCD3-APC and humanCD14-APC/Cy7 from Biolegend) and analyzed on LSRFortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
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bioRxiv - Microbiology 2021Quote: ... Cells were then filtered using a 40 μm cell strainer and stained with the Live/Dead dye 7 Amino-actinomycin D (7AAD, Biolegend) and antibodies against CD45.2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.