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Citations for BioLegend :
1351 - 1400 of 1916 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Immunology 2021Quote: Surface staining was performed using the following fluorescence-labeled antibodies against: CD4 (RM4-5; Biolegend, BD Biosciences), CD5 (53-7.3 ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 420 x g for 5 minutes and resuspended in anti-CD16/CD32 (Fc Block; Biolegend) diluted 1:50 in FACS buffer (PBS with 2% BSA and 1 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse Ly-6A/E (Sca1) (labelled with BV510) (BioLegend – 108129; 5 µl per million cells) and rat anti-lineage cocktail (labelled with BV421 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with antibodies diluted in a blocking solution of 5% Normal Goat Serum (BioLegend) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pelleted at 220-300 x g and resuspended in 5 mL RBC lysis buffer (BioLegend) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... C3b binding was detected with PE-conjugated mouse monoclonal anti-C3b/iC3b (5 μg/mL, BioLegend #846103) and C5b-9 membrane deposition/insertion was detected with mouse monoclonal anti-C5b-9 antibody (8 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... cytokines (hIL-10 20ug, mIL-15 15ug bid, IFN-γ 5×104 IU from BioLegend Inc., CA) were injected intraperitoneally in mice on day 1 ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining lysate (800 μl) was incubated with 5 μl of mouse anti-HA antibody (Biolegend #901514) with rocking for 4 hours at 4°C followed by addition of 200 μl of magnetic beads (Pierce #88803 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a secondary incubation with 5 µg/mL anti-HA-Alexa Fluor 647 (clone 16B12, BioLegend) and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... All samples were then blocked with 5 μg/mL anti CD16/32 (93; BioLegend Cat# 101301, RRID:AB_312800) and heat-inactivated normal mouse serum (1:10 ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 5-10 µg/ml) or purified KPL-1 anti-PSGL-1 mAb (cat. no. 328802, Biolegend). The PL1 hybridoma developed by McEver ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were treated for 5 min with TruStain FcXTM (anti-mouse CD16/CD32) (cat. #101320, BioLegend) prior to staining ...
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bioRxiv - Immunology 2023Quote: ... supernatants from cell cultures were harvested for detecting cytokine using LEGENDplexTM Human Th1 Panel (5-Plex) (BioLegend). Samples were diluted 5-fold using assay buffer and then mixed with beads and shaken for 2 hours at 500 rpm in a 96 plate well ...
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bioRxiv - Microbiology 2023Quote: ... parasites were stained with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Cell Division Tracker Kit, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Immunology 2023Quote: Following primary antibodies were used for immunofluorescence microscopy: anti-CD4 AF 647 (clone RM4-5, Biolegend #100530), anti-CD8α (clone EPR21769 ...
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bioRxiv - Immunology 2023Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...
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bioRxiv - Immunology 2024Quote: Surface staining for flow cytometry: PE-Cy7 rat anti-mouse CD4 (clone RM4-5, BioLegend, cat# 100528) (48) ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and incubated with fluorescently labeled antibodies (CD4 PE, clone RM4-5, 1:400, BioLegend; CD8a FITC ...
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bioRxiv - Immunology 2024Quote: ... Plates for CD8+ T-cell culture were pre-treated with 5 ug/mL α-CD3 (Biolegend, 100239) in phosphate buffered saline for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse interleukin (IL)-4 PE (504104) and anti-mouse IL-17 PE (517008) (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Samples were next stained for surface markers for 20min at 4°C with: PerCP-Cy5.5-conjugated anti-TCRβ (H57–597, BioLegend), APC-CY7-conjugated anti-B220 (RA3-6B2 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... cells were cultured overnight with or without 4 μg/mL Syrian hamster anti-mouse CD28 (clone 37.51, BioLegend, USA) and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Cell Biology 2020Quote: After coating with poly-L-Lysine, fluorodishes were coated overnight at 4 °C with αCD3ε (OKT3, #16-0037-85, eBioscience) + αCD28 (CD28.2, #302914 Biolegend), washed three times and pre-warmed at 37 °C for 10–15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min at 4°C with a fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 Ab (BioLegend). The cells were then fixed ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Immunology 2020Quote: ... then stained 15 minutes for surface markers and fixed for 20 minutes with 4% paraformaldehyde (PFA) in PBS (BioLegend). Cells were then permeabilized with 0.5 % saponin in Running buffer and stained for intracellular markers ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μL of WCE was incubated overnight at 4°C with 10μg of monoclonal RNA Poll antibody (Biolegend, USA), 2μg of anti-myc antibody (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained at 4°C on an agitation stage overnight with anti-CD169 and anti-Ly6G antibodies (BioLegend). Stained sections were microscopically analyzed using Leica SP8 confocal microscope (Leica Microsystem ...
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bioRxiv - Cancer Biology 2022Quote: ... we collected the beads and labeled them with 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6) for 45 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Genomics 2024Quote: ... The sorted cells were centrifuged at 400xg for 5mins at 4°C and re-suspended in 25μl Cell Staining Buffer (BioLegend). 2.5μl of 1:10 TruStain FcX™ PLUS (antimouse CD16/32 ...