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Citations for BioLegend :
1301 - 1350 of 8287 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Genomics 2024Quote: ... A subset of the iPSC lines was evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2024Quote: ... anti-SHIP-1 (P1C1-A5, PE), human IgE (MHE-18, Alexa Fluor 647), HLA-DR (L243, APC-Cy7) (all from Biolegend); and phospho-p65 NF-κB (K10-895.12.50 ...
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bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.
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bioRxiv - Immunology 2019Quote: ... 0.2% BSA in PBS with 0.02% sodium azide), blocked with human (humans, NHPs) or mouse (mice) TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Immunology 2021Quote: ... IL-10 in human cell culture supernatants were measured using the LEGEND Max Human IL-10 ELISA kit (BioLegend). Technical duplicates were averaged and then normalized to the total number of live cells in each sample at the time of collection.
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD64 FITC-conjugated (clone 10.1) and monoclonal mouse IgG2a anti-human CD59 PE-conjugated were from BioLegend, San Diego ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 420 x g for 5 minutes and resuspended in anti-CD16/CD32 (Fc Block; Biolegend) diluted 1:50 in FACS buffer (PBS with 2% BSA and 1 mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... The nonspecific binding of Fc receptors was blocked using anti-CD16/32 antibody (BioLegend, catalog no. 101302) on ice for 30 min ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend), and Brilliant Stain Buffer (BD Bioscience) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were stained with Zombie-NIR followed by Fc block with anti-mouse CD16/32 antibody (Biolegend). Cells were then stained for cell surface markers as described above ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Fc-Block was performed (10µg/ml purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse), after which cells were stained with Fixable Viability Staining e780 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with Fc block TruStain FcX (CD16/32) to block nonspecific FcR binding (#422302, Biolegend). To detect MHC-I and MHC-II ...
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bioRxiv - Neuroscience 2022Quote: ... Fc receptors were then blocked with an anti-CD16/CD32 antibody (BioLegend, San Diego, CA, U.S.A., #553141). Cells were washed and then incubated with conjugated antibodies (FITC-CX3CR1 ...
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bioRxiv - Immunology 2023Quote: ... Nonspecific binding was blocked by incubating cells anti-mouse CD16/32 Fc block (Biolegend; cat. no. 101310). Sytox-Green viability dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in washing buffer (PBS 0.04% BSA) and incubated with mouse Fc blockers (FcX, Biolegend). Each sample was then incubated with either TotalSeq anti-mouse Hashtag Antibody 1 (Biolegend #155861 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% NaN3 in 1X PBS) and blocked with Fc receptor antibody specific for FcyR III/II (Biolegend TruStain FcX PLUS anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in semi-facs buffer in the presence of Fc receptor blocking antibody (BioLegend, 101302) and stained with antibodies at a 1:200 dilution with the myeloid or lymphoid antibody cocktails ...
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bioRxiv - Bioengineering 2023Quote: ... CAR-M cells was prepared and the Fc receptor was blocked with anti-moCD16/32 antibody (BioLegend) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cells that were used in CITE-seq experiments were blocked with TruStain Fc block (BioLegend; Cat. 422301) for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Biophysics 2022Quote: ... Monoclonal antibodies against human Glycophorin AB (CD235ab) (HIR2; catalog number: 306602) and human CD47 (CC2C6; catalog number: 323102) were purchased from BioLegend.
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
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bioRxiv - Immunology 2020Quote: ... human Interlukin-6 (IL-6) (100 ng/ml) and human IL-6Rα (100 ng/ml) (BioLegend, San Diego, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... FITC anti-human CD18 antibody and FITC anti-human CD11c antibody (clone Bul15) were purchased from BioLegend (San Diego, USA). Rabbit anti-human MERTK antibody (D21F11 ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend ...
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bioRxiv - Microbiology 2024Quote: CD14+ monocytes were isolated from PMBCs of healthy human blood using the MojosortTM Human CD14 Selection Kit (BioLegend, Cat# 480026) To generate monocyte derived dendritic cells (moDCs) ...
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bioRxiv - Microbiology 2020Quote: ... stained with anti-human CD14 (Clone 63D3; Biolegend) and quantified using Attune NxT (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... BV421 anti-human CD14 (325627, clone HCD14, BioLegend), PE-Cy7 anti-human CD123 (560826 ...
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bioRxiv - Cell Biology 2020Quote: ... FITC anti-human Fas/CD95 (Clone DX2, Biolegend), APC anti-human Nectin-2/CD112 (Clone TX31 ...
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bioRxiv - Immunology 2022Quote: ... and the MojoSort Human CD8 T cell (BioLegend) isolation kit ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-human CD133 and anti-ICAM1 antibodies (BioLegend). A549 and H460 cells pretreated with DMSO ...
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bioRxiv - Bioengineering 2020Quote: ... or PE-Cy7 anti-Human CD117 antibody (Biolegend) then surface c-Kit intensity was read by flow cytometry ...