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Citations for BioLegend :
1301 - 1350 of 2082 citations for 6 Ethylthieno 2 3 d pyrimidin 4 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse P2ry12 (cat # 848006, BioLegend, clone S16007D, 2 ng/μl), BV421 rat anti-mouse CD45 (cat # 103133 ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were labeled with CFSE (Supporting Information Table 2, Biolegend, San Diego CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Pooled 8:2 CD45+:CD45-cells were resuspended in Cell Staining Buffer (BioLegend) and stained with a pool of 137 TotalSeq-C antibodies (Table ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Toll-like receptor 2 (anti-TLR2; Clone T2.5 for flow cytometry, Biolegend 121810 ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with anti-mouse CD14 rat IgG2a (clone Sa14-2; BioLegend) for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... H2-Dd-FITC (1:200; clone 34-2-12; BioLegend Cat. No. 110606), H2-Kd-APC (1:160 ...
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bioRxiv - Immunology 2024Quote: ... washed twice HBSS containing 2% FCS and incubated with Human Trustain Fcx (Biolegend) for 20 min on ice ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/ml anti-mouse-IgM or IgG (Biolegend) in NaHCO3 0.1 M pH 9.6 ...
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bioRxiv - Biophysics 2024Quote: ... 100 µL of PE donkey anti-rabbit IgG (BioLegend #406421; 2 ug/mL) for GST-LANA ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µL of PE goat anti-mouse IgG (BioLegend #405307; 2 ug/mL) added and incubated in the dark for 30 min with shaking (800 rpm) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 stimulation group (LN stim) with hashtag 4 (BioLegend, Cat# 155807); lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper ...
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bioRxiv - Genetics 2019Quote: ... anti-CD25-PE (8 µl) and anti-CD45RA-BV785 (4 µl, clone HI100, Cat. no. 304140, BioLegend), incubated at RT in the dark for at least 30 min. ...
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bioRxiv - Bioengineering 2021Quote: ... which were incubated overnight at 4 °C with primary antibodies diluted 1:5,000 in blocking buffer (BioLegend Way ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend) monoclonal anti-CD10 ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were cultured for 4 hours in the presence of PMA and Ionomycin (Cell Stimulation Cocktail, Biolegend) and Brefeldin A (Biolegend) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse CD8a (Biolegend, PE/Cy5), CD4 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... stained for 30 minutes at 4℃ with the following antibody: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend), PE/Cyanine7 anti-mouse CD4 antibody (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... intracranial and systemic lymphocyte samples were stained with anti-human CTLA-4 antibody (Biolegend, clone BNI3, BV421). Additionally ...
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bioRxiv - Immunology 2020Quote: The isolated cells were then stained for 30 min at 4°C with the following antibodies (Biolegend): APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for CTLA-4 were treated with Cell Activation Cocktail without Brefeldin A (Biolegend, Cat #423302) for 4 hours prior to fixation ...
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bioRxiv - Cell Biology 2021Quote: ... were blocked with 5ul Human TruStain FcX™ Blocking Reagent for 10 minutes at 4°C (BioLegend). 1ug of each TotalSeq™-A antibody was added to the cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained for 30 min at 4°C with FITC-conjugated anti-human CD3 antibody (Biolegend), followed by fixation and permeabilization of cells with BD Cytofix/Cytoperm™ (BD Bioscience) ...
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bioRxiv - Immunology 2022Quote: ... at 4°C) and stained with a fixable viability dye (Zombie NIR™ Fixable Viability Kit, Biolegend) in PBS for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11 ...
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bioRxiv - Immunology 2022Quote: ... Samples were incubated overnight at 4°C with anti-CD15(HI90)-Bi209 (primary antibody purchased from Biolegend; conjugated with Fluidigm’s MaxPar labeling kit ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...
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bioRxiv - Immunology 2023Quote: ... 4°C) the cells were stained with fluorochrome-conjugated antibodies for FACS Analysis (all antibodies from Biolegend) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse GL7 (Biolegend, Pacific Blue), IgM (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 5 min at 4°C with biotinylated anti-CD19 antibody (1D3; BioLegend) to exclude CD19-positive B cells in the cell suspension using anti-biotin microbeads and LS columns ...
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bioRxiv - Immunology 2023Quote: ... for 10min at 4°C, then FcR (Miltenyi, Cat. 130-092-575) and monocyte (Biolegend, Cat. 2000135006) blocking for 10min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated at 4°C overnight in primary antibodies against anti-HA (1:100; BioLegend; 901533), CA3 (1:150 ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed for 5 to 10 min at room temperature in 4% paraformaldehyde fixation buffer (BioLegend). Cells were permeabilized for 30 min at 4°C in PhosFlow Perm Buffer III (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Staining with primary antibodies was performed overnight at 4°C in blocking buffer (LN: IgD (Biolegend, 405705), and CD8 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... wells were incubated overnight at 4°C with mouse anti-β-III-tubulin (1:500, Biolegend, #MMS435P). After washing with PBST ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Systems Biology 2021Quote: ... deeply anesthetized mice (5% isoflurane) were retro-orbitally injected with 3 μg of anti-CD45 antibody (PerCP, clone 30-F11, Biolegend) 3 min prior to euthanasia [46,81] ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...