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Citations for BioLegend :
1301 - 1350 of 7866 citations for 6 Chloro 2 3 diphenylimidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... TH17 skew media contained IL-6 (20ng/mL; BioLegend, 575704), IL-23 (10ng/mL ...
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bioRxiv - Immunology 2024Quote: ... We stimulated T cells with human recombinant IL-6 (BioLegend), IL-15 ...
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bioRxiv - Immunology 2024Quote: ... and anti-mouse TotalSeq-A Hashtag antibody (4-6; BioLegend). The enriched samples were then stained with APC-labeled Streptavidin (BioLegend ...
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bioRxiv - Biochemistry 2024Quote: ... Direct-Blot HRP anti-6×His Epitope Tag antibody (BioLegend) at 1:10,000 ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes and ascitic cells were isolated and re-stimulated with SIINFEKL peptide (immunodominant ovalbumin peptide) for 12 hours in the presence of 2 uM Monensin (BioLegend, San Diego, CA) with the following intracellular staining of IFN-γ and measurement of IFN-γ-producing CD8+ T cells (meaning ovalbumin-specific T cells ...
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bioRxiv - Immunology 2022Quote: ... before staining with anti-mouse Granzyme B-efluor 450 and anti-mouse IFNγ-PE (BioLegend). Brain cells isolated from Pba-infected mice injected with BrdU were stained using the FITC BD BrdU Flow Kit (BD Pharmingen™ ...
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bioRxiv - Immunology 2019Quote: ... and GC B cells were stained with antibodies against mouse B220 (Clone: RA3-6B2, Biolegend), GL7 (Clone ...
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bioRxiv - Immunology 2021Quote: ... B cells were also stained with following anti-human antibodies: anti-IgD-BV421 (Biolegend, 348226), anti-CD27-FITC (BD biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... PE-HLA-A,B,C antibody (311406) and isotype control (400214) were purchased from BioLegend. Data were analyzed in FlowJo.
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bioRxiv - Cell Biology 2021Quote: ... Cells from three small and three large tissues were stained with TotalSeq-B Antibodies (Biolegend) for CITE-seq analysis ...
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bioRxiv - Cancer Biology 2022Quote: HLA surface expression was assessed using pan-HLA-A/B/C antibody (BioLegend, W6/32), anti-HLA-A03 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and stained with fluorescently-conjugated antibodies against HLA-A/B/C (clone W6/32; BioLegend), HLA-B ...
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bioRxiv - Immunology 2021Quote: ... confluent BCR-expressing B cells were FACS sorted on IgM (APC anti-human IgM; BioLegend) and kappa light chain (PE anti-human kappa light chain ...
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bioRxiv - Immunology 2022Quote: ... Nalm6 and primary B cells were labeled with PE/Cy7 anti-human CD19 Antibody (Biolegend) and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2 ...
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bioRxiv - Immunology 2024Quote: ... B-mercaptoethanol and Pen/Strep in the presence of 100ng/ml M-CSF (576406, Biolegend) followed by 3 more days with renewed media and cytokines ...
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bioRxiv - Cancer Biology 2023Quote: ... Alexa Fluor 647 or BV421-conjugated Granzyme B (BioLegend Cat# 515405, BD Biosciences Cat#563389); FITC-conjugated Foxp3 (Thermo Fisher Scientific Cat# 11577382) ...
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bioRxiv - Cell Biology 2023Quote: ... as well as 0.5 ug each of the 10 TotalSeq-B oligo-tagged antibodies (BioLegend) listed in Supplementary Table 3 were added and cell pellets were suspended ...
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bioRxiv - Neuroscience 2024Quote: ... To 4µl of each hashtag antibody (Hashtag B 301#, 0.5 mg/ml (BioLegend, Cat#: 155831); Hashtag B 302# 0.5 mg/ml (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Cells from three small and nine large tissues were stained with TotalSeq-B Antibodies (Biolegend) for CITE-seq analysis ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Galectin-3-AF647 (125408) from BioLegend and anti-CHMP4B (13683-1-AP ...
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bioRxiv - Immunology 2022Quote: ... and 3 μg/ml anti-CD28 (Biolegend) in RPMI with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... and rTGFβ (3 ng/ml; Biolegend, #580706), with or without 2.5μM mCRAMP ...
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bioRxiv - Immunology 2020Quote: ... and rTGFβ (3 ng/ml; Biolegend, #580706), with or without 2.5 μM mCRAMP for 1 to 3 days ...
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bioRxiv - Immunology 2021Quote: ... [clone 1D4B (BioLegend)] and CD107b (Mac-3) [clone M384 (BioLegend)].
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bioRxiv - Genomics 2022Quote: ... CD55 (PE-conjugated; clone RIKO-3; Biolegend), CD41 (APC-conjugated ...
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bioRxiv - Immunology 2022Quote: ... FITC-TIM-3 (clone F38-2E2, BioLegend), FITC-LAG-3 (clone 11C3C65 ...
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bioRxiv - Immunology 2024Quote: ... anti TIM-3-BV605 (RMT3-23, Biolegend), anti CD44-PE-Cy7 (IM7 ...
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bioRxiv - Immunology 2022Quote: ... and anti-mouse LAG-3 (C9B7W, Biolegend), which were stained for 60 min at 37°C as previously described (Gagliani et al. ...
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bioRxiv - Immunology 2023Quote: ... IgG2b BV605 or R718 (R12-3, BioLegend), IL-7Rα BV605 or BV421 (AFR34 ...
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bioRxiv - Immunology 2023Quote: ... Panel 3) CD3-AF700 (Biolegend Cat. 300323), CD4-APC Fire750 (Biolegend Cat ...
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bioRxiv - Neuroscience 2024Quote: ... β3 tubulin (mouse, BioLegend, TUJ1, #MMS-435P), myc (rabbit ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.