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Citations for BioLegend :
1301 - 1350 of 1916 citations for 3 Bromo 4 chloro 5 methoxypyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... for 15 minutes at 4°C followed by incubation with AntiHA.11 Epitope Tag Affinity Matrix (BioLegend 900801) for 4 hours at 4°C under constant rotation ...
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bioRxiv - Immunology 2024Quote: ... in FACS buffer for 30 minutes at 4°C prior to being stained with Zombie NIR (Biolegend, #423105) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured for 4-hours and then washed and stained with Zombie RedTM Fixable Viability dye (BioLegend). CountBright Absolute Counting Beads (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and stained for an additional 30 min at 4° C either with biotinylated anti-CD16 (clone 3G8, BioLegend) or anti-CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2024Quote: ... and cells were surface stained at 4°C for 20 minutes: anti-F4/80:FITC (Biolegend, clone BM8), anti-CD64:PerCP-Cy5.5 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Surface markers were then stained for 20 minutes at 4°C with antibodies against TCRb-Fitc (Biolegend #109205), TCRb-PE-Cy7 (Biolegend #109221) ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated overnight at 4 °C with either α-PS1 NTF antibody (1:2000; Biolegend PS1 NT1 823401) or α-PS2 NTF antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were stained in suspension for 30 min at 4°C with anti-CD31-AlexaFluor 488 (Biolegend, 303110), anti-CD274-PE (BD ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated at 4°C for 1h in an antibody cocktail containing anti-CD11b (PE, Biolegend, 101207), anti-CD11c (Brilliant Violet 421 ...
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher], rabbit polyclonal anti-tubulin β-3 IgG at 1:500 [Biolegend]) in PBS containing 1% Triton X-100 for 48 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Every 106 B cells in 100 μl solution were stained with 3 μl FITC antihuman CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μl Brilliant Violet 421 antihuman CD27 antibody (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... Linker oligo with 3’-terminal biotin was purchased from IDT (Coralville, Iowa) and purified streptavidin was purchased from BioLegend (Biolegend, #405150). Biotinylated antibody ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were isolated and stimulated for 48 h with 3 μg/ml plate-bound anti-mouse CD3 antibody (100340, BioLegend, USA) and 2 μg/ml soluble anti-mouse CD28 antibody (102116 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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bioRxiv - Bioengineering 2024Quote: ... hiPSC-CMs were dissociated by enzymatic digestion (Miltenyi Multi Tissue dissociation kit 3) and stained with the Zombie NIR™ Fixable Viability Kit (BioLegend). Then ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: To determine galectin-3 or galectin-8 expression the following: intracellular protein and lysosomes were stained using anti-Lamp1-BV421 (clone H4A3, BioLegend, USA), anti-Galectin-3-AF488 (clone B2C10 ...
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bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% triton in PBS) for 3 hours then incubated with Alexa Fluor 594 anti-β-amyloid (Clone 6E10; 1:1,000; BioLegend; 803019), Olig2 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... diluted in PBS with NHS and Triton X (both at 1%): 1) mouse isotype IgG2a anti-Tubulin beta 3 (TUJ1, 1:1000, BioLegend #801201), 2 ...
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bioRxiv - Neuroscience 2024Quote: ... and preMG were resuspended in 3:1-medium supplemented with 50 ng/mL recombinant human MCSF (Macrophage Colony Stimulating Factor, BioLegend, #574804). Cells were counted using an automated cell counter (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The cell suspension was distributed over a 96-well plate (200 μL/well) that had been precoated with 3 μg/mL anti-CD3ε (Biolegend, #1000340) for 2 h ...
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bioRxiv - Immunology 2021Quote: ... 1-5 * 106 cells were stained with antibodies specific for surface markers Thy1.2 (clone 30-H12, BioLegend), Thy1.1 (clone OX-7 ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies diluted in blocking solution: 5% Normal Goat Serum (Biolegend, 927502), or 0.2% (w/v ...
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bioRxiv - Immunology 2021Quote: ... An aliquot of cells (5 ×105) was labeled with a viability dye (BioLegend Zombie Aqua; Cat# 423101) and fluorochrome- conjugated antibodies for enumerating of activated T cells that expressed high levels of CD44 ...
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bioRxiv - Immunology 2021Quote: ... The cell were stained with 5 µl of PerCP/cy5.5-Armenian Hamster-IgG (Biolegend, USA, cat#400931), PE-Rat-IgG2a (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Immunology 2021Quote: Surface staining was performed using the following fluorescence-labeled antibodies against: CD4 (RM4-5; Biolegend, BD Biosciences), CD5 (53-7.3 ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 420 x g for 5 minutes and resuspended in anti-CD16/CD32 (Fc Block; Biolegend) diluted 1:50 in FACS buffer (PBS with 2% BSA and 1 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse Ly-6A/E (Sca1) (labelled with BV510) (BioLegend – 108129; 5 µl per million cells) and rat anti-lineage cocktail (labelled with BV421 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with antibodies diluted in a blocking solution of 5% Normal Goat Serum (BioLegend) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pelleted at 220-300 x g and resuspended in 5 mL RBC lysis buffer (BioLegend) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... C3b binding was detected with PE-conjugated mouse monoclonal anti-C3b/iC3b (5 μg/mL, BioLegend #846103) and C5b-9 membrane deposition/insertion was detected with mouse monoclonal anti-C5b-9 antibody (8 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... cytokines (hIL-10 20ug, mIL-15 15ug bid, IFN-γ 5×104 IU from BioLegend Inc., CA) were injected intraperitoneally in mice on day 1 ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining lysate (800 μl) was incubated with 5 μl of mouse anti-HA antibody (Biolegend #901514) with rocking for 4 hours at 4°C followed by addition of 200 μl of magnetic beads (Pierce #88803 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a secondary incubation with 5 µg/mL anti-HA-Alexa Fluor 647 (clone 16B12, BioLegend) and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... All samples were then blocked with 5 μg/mL anti CD16/32 (93; BioLegend Cat# 101301, RRID:AB_312800) and heat-inactivated normal mouse serum (1:10 ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 5-10 µg/ml) or purified KPL-1 anti-PSGL-1 mAb (cat. no. 328802, Biolegend). The PL1 hybridoma developed by McEver ...