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Citations for BioLegend :
1301 - 1350 of 1708 citations for 2S 4S 4 Fmoc amino pyrrolidine 2 carboxylic acid methylester hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Immunology 2020Quote: ... or 2) 10 μg/mL αLFA-1 + 10 μg/mL antibodies against CD16 (αCD16; BioLegend, clone 3G8). All loading solutions were mixed in PBS and supplemented with 10 μg/mL bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 2 times in 1x Perm/Wash buffer and resuspended in Cell Staining Buffer (Biolegend) prior to flow cytometry analysis ...
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bioRxiv - Genomics 2024Quote: ... at a 1:300 dilution with DAPI 2 μl in 1 ml of 10 μg/ml (BioLegend) and kept on light rotation for 2 h at RT ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend, 422302) in PBS ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used at a concentration of 1:100: CD14 (BioLegend, #123312, clone Sa14-2), CD86 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and penicillin in a 96-well plate pre-coated with anti-CD3Ab (2 μg/ml, Biolegend 100360), along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122 ...
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bioRxiv - Cell Biology 2023Quote: ... 1–2 million cells/100 μl were incubated with the following antibodies: anti-CD11c PeCy7 (117318, Biolegend), anti-CD11b (101212 ...
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bioRxiv - Immunology 2023Quote: ... aliquots of conditioned media were collected for measuring IL-2 and IFNγ by ELISA (ELISA MAX, BioLegend). Cells were washed and stained with anti-mouse CD3ε-FITC (145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... serum samples were diluted 2-fold with assay buffer and standards were mixed with matrix solution (Biolegend) to account for additional components in the serum samples ...
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bioRxiv - Immunology 2023Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed with 2% paraformaldehyde (Pierce, 28906) diluted in PBS or commercial fixation buffer (Biolegend, 420801) for 20 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated for 2 days with 10 ug/ml of anti-CD40 (Biolegend, Cat#102812) and 10 ng/ml of rec ...
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bioRxiv - Immunology 2022Quote: ... in the presence of rhIL-2 (50U/ml, Novartis) and anti-IFNγ (5µg/ml, clone XMG1.2, Biolegend). For intracellular cytokine staining ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: ... cells were activated at ∼2-4M cells/ml for 8 hrs using CD3 antibody (Biolegend clone OKT3) coated tissue culture plates ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2) (Biolegend, San Diego CA) viability dye and blocked using 2% FBS ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31 ...
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bioRxiv - Genetics 2024Quote: ... MDA-MB-231 and B80-T5 by flow cytometry (Biolegend, PE anti-human CD340 (erbB2/HER-2) Antibody ...
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bioRxiv - Microbiology 2024Quote: ... HEPES (10 mM) and L-glutamine (2 mM) supplemented with 25 ng/mL M-CSF (Biolegend, 574806) for 6 days.
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bioRxiv - Molecular Biology 2024Quote: ... Red blood cells were lysed using a 2-minute incubation with RBC lysis buffer (BioLegend, catalog #420302) and the cells were then washed ...
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bioRxiv - Immunology 2024Quote: ... eleven different TotalSeq™-C anti-human Hashtag oligo-antibodies (0.5 g/106cells; BioLegend, Supplementary table 2) were added to the PBMCs stimulated with myelin peptides to enable further multiplexing of the sorted samples at the single-cell TCR sequencing step ...
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bioRxiv - Genetics 2024Quote: ... they were stained with 200 ng/1 million cells PE CD46 (TRA-2-10) flow antibody (BioLegend) and the CD46 negative population was separated by fluorescence-activated cell sorting ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Bioengineering 2022Quote: ... For immune cells detections the tumoroids sections also were incubated overnight at 4°C with anti-B220 (Biolegend 103224, APC-cy7 conjugated) and anti-cd11b (Biolegend 101208 ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... supernatant was aspirated and the cells were incubated in blocking buffer mixture for 5min at 4°C (1uL anti-mouse CD16/32 TruStain Fcx (RRID: AB-1877271 AB-1574973, Biolegend Cat #: 101320) and 99uL FACS buffer (1% BSA + 0.05% sodium azide in HBSS w/o Ca2+/Mg2+)) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation (1500rpm; 5mins; 4°C) and washed twice with Annexin V binding buffer (Biolegend; 1ml per wash). The cell pellets were resuspended in of binding buffer (100μl ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and stained in PermWash buffer (eBioscience) for 30 min at 4°C with Abs (purchased from BioLegend unless noted) against Bcl2 (BCL/10C4) ...
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bioRxiv - Immunology 2021Quote: ... Cell surface proteins were stained for 20 min at 4 °C with the following antibodies: CD45.1 (BioLegend, 110714, Clone ID: A20, RRID:AB_313503), CD45.2 (BioLegend ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Stain 510 for 15 minutes at 4 °C followed by anti-Fc blocking reagent (Biolegend, Cat# 101320) for 10 minutes prior to surface staining ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend, clone 17A2), CD44 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with 1x Perm buffer (Tonbo) and stained for intracellular antigens for 30 mins at 4°C with the following antibodies: GranzymeB: AF647 (Biolegend, Clone: GB11), IL-2 ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...