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Citations for BioLegend :
1301 - 1350 of 7957 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with 2 mL of PBS + BSA (400 g for 5 min) and run fixed (Biolegend fixation buffer) or unfixed prior to sorting and analysis ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm Staining Buffer (SB; Biolegend #420201). Cells were washed twice at 300 g for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm staining buffer (SB; Biolegend #420201) or media buffer (Thermo Fisher Scientific DMEM #12634010 or RPMI #61870010 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... EBs were plated on Geltrex™-coated 6-well plates (10 EBs/well) in APEL™2 medium supplemented with VEGF (40 ng/ml) (BioLegend), SCF (50 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... AML cell lines and PBMCs from healthy individuals and AML patients were stained with HERV-K102 Env-TM autoantibody and the secondary antibody PE F(ab’)2 Goat anti-human IgG Fcγ (BioLegend, Cat 398004), as well as PE Mouse IgG1 ...
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bioRxiv - Neuroscience 2024Quote: ... CTPI-2 toxicity was assessed by treating assembloids with 50uM CTPI-2 for 24hours prior to dissociation and staining with an Annexin V Cell Viability assay (Biolegend #422201 & #640935) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... were coated overnight at 4 °C with 100 μl Tau-12 capture antibody per well (2 μg/ml; Biolegend, cat. no 806502) in 20 mM NaH2PO4 pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with 0.5μg of one of eight surface hashing antibodies (Biolegend) so that their sample of origin could be identified following sequencing across two lanes as previously described (Kendal AR 2020).
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bioRxiv - Immunology 2023Quote: ... One sample received an irrelevant antibody (a mouse IgG1k against IFNγ from Biolegend) control ...
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bioRxiv - Neuroscience 2023Quote: ... for one hour at room temperature and were stained with vimentin (#677809, Biolegend), cleaved Caspase 3 (#9604S ...
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bioRxiv - Cancer Biology 2023Quote: ... Then one million cells were blocked with an anti-CD16/32 antibody (BioLegend) for 10 min at 4 °C followed by staining with the following appropriate antibodies for 30 min at 4 °C ...
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bioRxiv - Genetics 2024Quote: ... One million cells were stained with Zombie Violet Fixable Viability Kit (423113, BioLegend) for 30 min at 4 °C prior to fixation and permeabilization (FOXP3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Synthetic Biology 2024Quote: ... supernatant samples were collected to measure IFN-γ and IL-2 using commercial enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions (BioLegend, 430801 and 431801).
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bioRxiv - Microbiology 2024Quote: ... 2 X 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 μl of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (dilution 1:200 ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes and ascitic cells were isolated and re-stimulated with SIINFEKL peptide (immunodominant ovalbumin peptide) for 12 hours in the presence of 2 uM Monensin (BioLegend, San Diego, CA) with the following intracellular staining of IFN-γ and measurement of IFN-γ-producing CD8+ T cells (meaning ovalbumin-specific T cells ...
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bioRxiv - Cell Biology 2023Quote: ... These coverslips were then washed with PBS containing 0.025% Tween 20 and EVs were labeled with one of the following affinity reagents: 1) a mixture of AF647-labeled antibodies specific for mouse CD9 (Biolegend, Cat. No. 124802, San Diego, CA, USA), mouse CD63 (Biolegend ...
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bioRxiv - Genomics 2022Quote: ... one million PBMCs were resuspended in 47.5 μL of Cell Staining Buffer (BioLegend #420201) and 2.5 μL TruStain FcX block (BioLegend #422301 ...
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bioRxiv - Neuroscience 2024Quote: ... one series was processed using the monoclonal antibody SMI-32 (Biolegend; Sternberger & Sternberger 1983) to allow for laminar and areal delineation of auditory cortical subfields ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2021Quote: ... The cells were stimulated with 20μg/ml of a purified F(ab’)2 goat anti-mouse IgM (μ chain) antibody (BioLegend, Clone Poly21571, Cat# 157102) at 37°C for 10 seconds ...
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bioRxiv - Immunology 2022Quote: ... 5 x 105 cells from each sample were stained for combinatorial hashing using oligonucleotide-conjugated Hashtag antibodies targeting the ubiquitous cell surface antigens Beta-2-Microglobulin and CD298 (Biolegend Totalseq A Hashtag antibodies). Hashed samples were pooled and stained together with a cocktail of 73 oligonucleotide-conjugated antibodies targeting cell surface antigens ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 3ml flow buffer (PBS with 2% FBS) at 300xg and stained with goat anti-mouse IgG-APC (BioLegend, clone Poly4053, catalog # 405308) and rat anti-mouse IgM-PE (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: Tumor cell lines and murine splenocytes were stained for flow cytometry assays in FACS buffer (1X PBS, 2 mM EDTA and 2c% FBS) with the following antibody panel: CD3-BV510 (BioLegend, clone 17A2, cat. 100233), NK1.1-PE-Cy7 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million cells for 30 min at 4 °C (Cat. No. 394601, 394603, 394605 and 394661, 394663, 394665, respectively, Biolegend, San Diego, CA, USA). After 3 washes with PBS with 0.05 % BSA respective cell suspensions were mixed prior to single-cell RNA library preparation ...
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bioRxiv - Immunology 2023Quote: ... or one drop each of positive and negative Biolegend compensation beads (Biolegend, San Diego, USA) were diluted in 1ml of staining buffer (HBSS +2mM EDTA) ...
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bioRxiv - Developmental Biology 2023Quote: ... one uterine horn of XER mice was injected intraluminally with 1µg recombinant Lif (BioLegend, 554006) at 1300h on GD3 ...
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 million cells per sample were resuspended in CITE-seq staining buffer (2% BSA, .01% Tween in PBS) and incubated for 10 minutes with Fc receptor block (TruStain FcX, BioLegend and FcR blocking reagent, Miltenyi) to prevent antibody binding to Fc receptors ...