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Citations for BioLegend :
1251 - 1300 of 2115 citations for 7 Bromo 4 chlorothieno 3 2 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and CD8 (2 μg/ml APC/Cy7 a-mouse CD8a antibody; Biolegend) in PBS for 20 min before fixation in 2% paraformaldehyde (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... and IFN-γ (2 μg/ml APC a-mouse IFN-γ, Biolegend) prior to analysis on a flow cytometer (LSR Fortessa X-20 ...
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bioRxiv - Immunology 2021Quote: ... and anti-mouse-IL-2-PE-Cy7 (Biolegend Cat# 503832, RRID: AB_2561750). Stained cells were acquired by FACSAriaIII Flow Cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... and AlexaFluor-647-conjuaged anti-CD18 antibody (M18/2, Biolegend, 1:100). Cells were washed with FACS buffer ...
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bioRxiv - Pathology 2021Quote: ... pan aSyn (N-terminal directed, SYN303 [aa 2-12 Cat# 82401, Biolegend] ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 μg/ml soluble anti-mouse CD28 antibody (102116, BioLegend, USA) in RPMI 1640 medium (21870-076 ...
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bioRxiv - Immunology 2021Quote: ... PE-anti-human-Nectin-2 (TX31) (all from BioLegend, San Diego, CA). The following antibodies were used for in vivo blocking experiments ...
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bioRxiv - Immunology 2020Quote: ... KLRG1 APC-Cy7 (clone 2FI/KLRG1, BioLegend, cat# 138426, 2:100 dilution); CD279 (PD-1 ...
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bioRxiv - Immunology 2020Quote: ... CD279 (PD-1) BV650 (clone EH12.2H7, BioLegend, cat# 329950, dilution 2:100); CD45RA BV510 (clone HI100 ...
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bioRxiv - Immunology 2022Quote: ... 1-2×106 cells were incubated with Human TruStain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406 ...
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bioRxiv - Neuroscience 2022Quote: ... Fisher) along with 2 µl of live/dead cell stain (DRAQ7, BioLegend) per ml and then FACS sorted.
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bioRxiv - Immunology 2022Quote: ... Rat anti-CD138 antibody (rat anti-CD138, clone 281-2, Biolegend, 142502) was applied to each section and specific bounds were visualized using a Alexa Fluor488-conjugated anti-rat antibody (JacksonImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... anti-CD138 Alexa Flour 647 (1:80 dilution, clone 281-2, BioLegend), donkey anti-mouse IgG Alexa Fluor Plus 405 (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS; BioLegend) and incubated with PGP9.5 for 20 min at RT ...
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bioRxiv - Immunology 2024Quote: ... 1-2×106 cells were incubated with Fc receptor blocking reagent (BioLegend) for 5 min at RT ...
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bioRxiv - Immunology 2024Quote: ... and with 100 U/ml IL-2 (BioLegend, San Diego, CA, USA) (referred to as “Bead activated T cells” or “nonspecific activation” ...
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Immunology 2024Quote: Neutrophil phenotyping panel 2: Ter119-FITC (clone TER-119 Biolegend 1:200), NKp46-FITC (clone 29A1.4 Biolegend 1:200) ...
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bioRxiv - Immunology 2024Quote: ... Media was additionally supplemented with 10 ng/mL murine IL-2 (BioLegend) prior to resuspension and subsequent passaging ...
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bioRxiv - Immunology 2021Quote: ... prior to staining for 1 h at 4°C with the following antibodies from BioLegend (most) or Novus Biologicals (MARCO ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated overnight at 4 °C with the primary antibodies against ASC (HASC-71, BioLegend). Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20 ...
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bioRxiv - Genomics 2019Quote: ... cells were stained in batches of 1×107 cells for 30 min at 4°C (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation (“in vitro-derived”) were washed and labeled using CFSE (BioLegend). Murine bone marrow neutrophils were isolated by negative selection (StemCell Technologies ...
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bioRxiv - Genomics 2021Quote: ... This was followed by 30min incubation at 4 °C with a Streptavidin-PE (Biolegend, 1:5000), anti c-kit-APC (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... After 4 hours BMDMs were harvested and stained with an antibody against F4/80 (BM8, Biolegend) and propidium iodide (BD Biosciences) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse B220 (Biolegend, APC), GL7 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were incubated for 15 minutes at 4°C in streptavidin-PE/Cy7 (BioLegend) to detect NCAM-biotin ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were first incubated for 1h at 4°C with 2μl of anti- HA antibody (BioLegend) and then for another hr after adding 30μl of washed magnetic ProteinG beads (Cytiva) ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... Cells were then incubated for 10 minutes at 4°C with True stain Fc block (BioLegend) and washed ...
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bioRxiv - Immunology 2019Quote: ... macrophage activation was induced by adding either IL-4 (20 ng/ml, Biolegend, San Diego, USA), IL-13 (20 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by overnight staining at 4°C with rat anti-mouse CD45 primary antibody (103102, Biolegend) diluted 1:500 in 1x D-PBS ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... that had previously been linked with 4 μg each of mouse monoclonal 6E10 and 4G8 (Biolegend) were added to the neutralized FA fraction ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was removed by aspiration and 500 microliters of fixation buffer (4% paraformaldehyde in PBS, Biolegend) added to each well and the slide kept in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and stained (30 min at 4°C) with PE anti-TLR4 (Biolegend; clone Sa15-21; 145404) in blocking buffer (PBS with 2% rat serum) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed three times in FACS buffer and resuspended in FACS buffer containing 3 μg/ml DAPI (BioLegend). Flow cytometric analysis was done on a BD LSRFortessa analyzer and sorting was done on a BD FACSAria1 machine.
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2022Quote: ... mice were injected into the retro-orbital sinus with a total of 3 mg of PE/Cy7-CD45.2 (104, BioLegend) in 200 μl sterile DPBS ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...