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Citations for BioLegend :
1251 - 1300 of 7924 citations for 6 Amino 5 2 chloro 4 nitrophenyl azo naphthalene 1 sulfonamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Control antibodies were biotin-conjugated rat immunoglobulin (Ig) G1 (5 μg/mL; eBioscience, San Diego, CA) and PE/Cy7-conjugated rat IgG1 (5 μg/mL; BioLegend, San Diego, CA). Flow cytometry data were collected using Fortessa LSRII (Becton Dickinson ...
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bioRxiv - Immunology 2024Quote: ... 10 ug/mL) or LaAg (5 ug/mL) in the presence of a Golgi complex inhibitor Brefeldin A (5 mg/mL, Biolegend, SanDiego, CA, USA). All centrifugation steps were performed at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... The CD11a (LFA-1α chain) reactive monoclonal antibody TS2/4 (BioLegend) was conjugated with EZ-Link™ NHS-LC-LC-Biotin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with anti-mouse IL-4 antibody (11B11, Biolegend) then blocked as above ...
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bioRxiv - Immunology 2022Quote: ... and 4 μL of anti-CD193-PerCP/Cy5.5 (clone 5E8, BioLegend), and incubated the sample for ~18 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml IL-4 + 20 ng/ml IL-13 (Biolegend) for polarization to M2 ...
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bioRxiv - Systems Biology 2019Quote: ... for 20 minutes at 4°C and Zoombie NIR (BioLegend 423105) cell viability solution at 25 ng/ml (1:2,000 ...
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bioRxiv - Immunology 2020Quote: ... Bcl6 PE/Dazzle (clone 7D1, BioLegend, cat# 358510, dilution 4:100). Data was recorded on a FACSymphonyTM flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... IL-4 (11B11) and CD124 (I015F8) mAbs were purchased from BioLegend. Anti-CD44 (IM-7) ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4 times with washing buffer (BioLegend; Cat: 4211601) and then incubated for 1 h at RT with a 1:2000 dilution of secondary antibody of horse radish peroxidase (HRP)-conjugated rabbit anti-mouse IgM (μ chain) ...
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bioRxiv - Immunology 2020Quote: ... or 40 ng/mL IL-4 (Biolegend, San Diego, CA, USA) in duplicates respectively for 24 hours in X-VIVO 15 serum free medium ...
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bioRxiv - Immunology 2022Quote: ... and 10 μg/mL anti-IL-4 Ab (clone 11B11, BioLegend) were added to the culture media ...
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bioRxiv - Cancer Biology 2022Quote: ... we stained the cells with 4 μg/ml PE-streptavidin (BioLegend) at the 37 °C for 45 min ...
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bioRxiv - Immunology 2022Quote: Mouse splenocytes were stained with 4 μg/ml LAG3 BV785 (BioLegend) and 4 μg/ml CD49b PeCy7 (BioLegend ...
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bioRxiv - Pathology 2023Quote: ... Neutrophils were maintained 4 hours in culture with Brefeldin A (Biolegend) to stop exocytosis.
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% Triton-X and 4 µg/mL DAPI (#422801, BioLegend, USA) for 1 h at RT and stained with two staining panels ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde (Biolegend, San Diego, CA, USA) for 10 minutes and subsequently permeabilized with 0.1% Triton X100 (Merck KGaA ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cyanine7 anti-mouse IL-4 antibody (BioLegend, catalog no. 504118), and Brilliant Violet 711 anti-mouse IL-17A Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Cell Biology 2023Quote: ... TotalSeq™-A0304 anti-mouse Hashtag 4 Antibody) (BioLegend, TotalseqA antibodies). The 4 biological replicates of each time point were stained with one of the 4 unique hash antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... FITC anti-mouse CD4 Antibody (BioLegend, catalog #116003, clone RM4-4), PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-4 (#500808) was from BioLegend (San Diego, CA, USA). Cells detached from culture plates were filtered through 70-μm cell strainers ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...
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bioRxiv - Cancer Biology 2019Quote: ... IL-6 levels of culture supernatants were determined using the LEGENDplex™ Human Inflammation Panel I (BioLegend, 740809), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... The levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected using ELISA kits (Biolegend) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... mouse TNF-α (430904) and IL-6 (431304) enzyme-linked immunosorbent assay (ELISA) kits were purchased from BioLegend; Griess Reagent System (G2930 ...
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bioRxiv - Bioengineering 2021Quote: ... FITC anti-mouse H-2Kb/H-2Db Antibody (Clone 26-8-6) were from Biolegend (San Diego, CA). Primary antibodies of STING/TM173 (D2P2F) ...
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bioRxiv - Neuroscience 2023Quote: ... centrifugated (500g) and IL-6 secretion analyzed using a commercial ELISA kit following the manufacturer’s instructions (Biolegend, #431304).
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bioRxiv - Immunology 2022Quote: ... and two hashtag antibodies were built by combining the antibodies MHC I-Biotin (Biolegend, clone 28-8-6) and CD45-Biotin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... CD45-labelled cells were mixed (maximum 6 samples together) and stained with APC-conjugated FCRL5 antibodies (Biolegend #340305)(for panel #2) ...
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bioRxiv - Immunology 2023Quote: ... injections of a TH17 priming cocktail (n=8; 0.5μg rmIL-6 [575704, BioLegend, USA] and 1μg rmTGF-β1 [763104, BioLegend, USA]) or rest (n=8 ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was collected after 24 h to measure IL-6 and TNF-α levels using ELISA (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse CD63-160Gd (clone NVG-2, BioLegend), anti-mouse CD120b-166Er (clone TR75-32.4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...
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bioRxiv - Immunology 2020Quote: ... A647 anti-Bcl-2 (633510) (all from BioLegend); APC anti-CXCR4 (558644) ...
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bioRxiv - Immunology 2022Quote: ... anti-CD138 (BV711, 281-2, AB_2562571, BioLegend #142519), anti-CD19 (BV785 ...