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1201 - 1250 of 2737 citations for CD40 Human HEK293 His since 2019

• A 3D in vitro assay to study combined immune cell infiltration and cytotoxicity

  Ashleigh J Crawford, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Cell suspensions were blocked with Human TruStain FcX (Biolegend 422301) for 15 min under room temperature ...
• Cell of origin alters myeloid-mediated immunosuppression in lung adenocarcinoma

  Minxiao Yang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and anti-mouse/human CD11b-PE (1:200, 101207, Biolegend). The following isotype antibodies were diluted in FACS Buffer to be used in the MDSC surface staining panel ...
• Engineered 3D Hydrogel Matrices to Modulate Trophoblast Stem Cell-Derived Placental Organoid Phenotype

  Emily M. Slaby, et al., bioRxiv - Bioengineering 2024

  Quote: ... Human TruStain FcXTM (FC Receptor Blocking Solution, BioLegend catalog #422302) was incubated with the cells for 5 minutes at room temperature at a 1:250 dilution in DPBS with 1% BSA ...
• Hallmarks of tumor-experienced T cells are absent in multiple myeloma patients from diagnosis through maintenance therapy

  Carolyn Shasha, et al., bioRxiv - Immunology 2024

  Quote: ... Samples were thawed and stained with Human TruStain FcX (BioLegend) for 10 min at 25C ...
• Transcription of HIV-1 at sites of intact latent provirus integration

  Ana Rafaela Teixeira, et al., bioRxiv - Immunology 2024

  Quote: ... BrillianViolet conjugated anti-human CD25 (clone BC56, BioLegend, cat. 302634).
• In vitro spatiotemporal reconstruction of human skeletal muscle organogenesis

  Lampros Mavrommatis, et al., bioRxiv - Developmental Biology 2024

  Quote: ... and PE-labelled anti-human CD82 (Biolegend, clone ASL-24) antibodies and washed twice with 1% BSA staining solution ...
• Converging cytokine and metabolite networks shape asymmetric T cell fate at the term human maternal-fetal interface

  Nicholas J Maurice, et al., bioRxiv - Immunology 2024

  Quote: ... We stimulated T cells with human recombinant IL-6 (BioLegend), IL-15 ...
• Synergically enhanced anti-tumor immunity of in vivo CAR by circRNA vaccine boosting

  Yanyan Wang, et al., bioRxiv - Immunology 2024

  Quote: ... or PE/Cyanine7 anti-mouse/human CD11b Antibody (BioLegend, #101216), and incubated in the dark for 30 minutes at 4 °C ...
• LHPP expression in triple-negative breast cancer promotes tumor growth and metastasis by modulating the tumor microenvironment

  Jeffrey Reina, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and two human-specific markers HLA-ABC-FITC (BioLegend, 311404) and hCD44-PE (BioLegend ...
• Development of evolutionarily conserved viral integration sites as safe harbors for human gene therapy

  Marco A. Quezada-Ramírez, et al., bioRxiv - Genetics 2024

  Quote: ... The Fc receptors were blocked with Human TruStain FcXTM (BioLegend) before staining with antibodies against CD235a and CD69 for 20 min at 4°C in the dark ...
• CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses

  Sallieu Jalloh, et al., bioRxiv - Immunology 2022

  Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
• Engineering CAR-NK cells to secrete IL15 sustains their anti-AML functionality, but is associated with systemic toxicities

  Ilias Christodoulou, et al., bioRxiv - Immunology 2021

  Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
• Single cell reconstruction of human basal cell diversity in normal and IPF lung

  Gianni Carraro, et al., bioRxiv - Cell Biology 2020

  Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
• Autologous human immunocompetent white adipose tissue-on-chip

  Julia Rogal, et al., bioRxiv - Bioengineering 2021

  Quote: ... Cytokines were determined by fluorescent bead-based multiplex sandwich immunoassays (LEGENDplex™ Human Angiogenesis Panel 1, 740697 and LEGENDplex™ Human Adipokine Panel, 740196; BioLegend, Inc., San Diego, CA) read by flow cytometry (Guava easyCyte 8HT ...
• Versatile workflow for cell type resolved transcriptional and epigenetic profiles from cryopreserved human lung

  M Llamazares Prada, et al., bioRxiv - Cell Biology 2020

  Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend, Table S6) for 30 minutes on ice ...
• CD47 cross-dressing by extracellular vesicles expressing CD47 inhibits phagocytosis without transmitting cell death signals

  Yang Li, et al., bioRxiv - Immunology 2021

  Quote: ... APC-conjugated anti-human IgG Fc (clone HP6017) was from Biolegend. For analysis of CD47 expression on human PBMCs ...
• Potent but transient immunosuppression of T-cells is a general feature of CD71⁺ erythroid cells

  Tomasz M. Grzywa, et al., bioRxiv - Immunology 2021

  Quote: Recombinant human ARG1 was obtained from Biolegend (San Diego, CA, USA), recombinant murine Arg1 was obtained from Cloud-Clone Corp. ...
• Notch Signaling Commits Mesoderm to the Cardiac Lineage

  Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

  Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
• Patient-derived triple negative breast cancer organoids provide robust model systems that recapitulate tumor intrinsic characteristics

  Sonam Bhatia, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Alexa Fluor® 647 anti-human CD326 (EpCAM) Antibody (Biolegend 324212), PE anti-human/mouse CD49f Antibody (Biolegend 313612) ...
• Longitudinal Assessment of SARS-CoV-2 Specific T Cell Cytokine-Producing Responses for 1 Year Reveals Persistence of Multi-Cytokine Proliferative Responses, with Greater Immunity Associated with Disease Severity

  Jonah Lin, et al., bioRxiv - Immunology 2022

  Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD4 (BV711 ...
• Longitudinal Assessment of SARS-CoV-2 Specific T Cell Cytokine-Producing Responses for 1 Year Reveals Persistence of Multi-Cytokine Proliferative Responses, with Greater Immunity Associated with Disease Severity

  Jonah Lin, et al., bioRxiv - Immunology 2022

  Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD3 (APC-Cy7 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD123 BV510 (1:100, clone 6H6, BioLegend, cat # 306022), Anti-human CD34 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD69 BV650 (1:100, clone FN50, BioLegend, cat # 310934), Anti-human CD8a BV650 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human TCRgd BV510 (1:100, clone B1, BioLegend, cat # 331220), Anti-human TCRab BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD56 BV605 (1:50, clone HCD56, BioLegend, cat # 318334), Anti-human CD69 BV650 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD13 BV421 (1:50, clone WM15, BioLegend, cat # 301716), Anti-human TCRgd BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD3 BV510 (1:100, clone OKT3, BioLegend, cat # 317332), Anti-human CD14 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD3 FITC (1:100, clone UCHT1, BioLegend, cat # 300440), Anti-human CD8a PerCP (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD107a BV750 (1:50, clone H4A3, BioLegend, cat # 328638), Anti-human CD103 BV711 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human TCRgd FITC (1:100, clone B1, BioLegend, cat # 331208), Anti-human CD3 FITC (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD326 BV650 (1:100, clone 9C4, BioLegend, cat # 324226), Anti-human CD107a BV750 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CXCR3 BV421 (1:50, clone G025H7, BioLegend, cat # 353716), Anti-human CD13 BV421 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD20 BV510 (1:100, clone 2H7, BioLegend, cat # 302340), Anti-human CD3 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CCR6 APC (1:25, clone G034E3, BioLegend, cat # 353416), Anti-human CD206 APC (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD34 BV510 (1:100, clone 581, BioLegend, cat #343528), Anti-human CD20 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD45 BV711 (1:100, clone HI30, BioLegend, cat # 304050), Anti-human CD3 BV785 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CCR8 PE (1:100, clone L263G8, BioLegend, cat # 360604), Anti-human CD127 PE (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD14 BV510 (1:100, clone M5E2, BioLegend, cat # 301842), Anti-human CD19 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD19 BV510 (1:100, clone HIB19, BioLegend, cat # 302242), Anti-human CD4 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD5 BV510 (1:100, clone L17F12, BioLegend, cat # 364018), Anti-human FcER1 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD303 BV510 (1:100, clone 201A, BioLegend, cat # 354232), Anti-human CD123 BV510 (1:100 ...
• Simultaneous analysis of antigen-specific B and T cells after SARS-CoV-2 infection and vaccination

  Krista L. Newell, et al., bioRxiv - Immunology 2021

  Quote: ... biotin anti-human CD19 (BioLegend, San Diego, CA, USA, Cat. 302203) was used as a primary stain ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD10 BV711 (1:100, clone HI10a, BioLegend, cat # 312226), Anti-human CD45 BV711 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human TCRab BV510 (1:100, clone IP26, BioLegend, cat # 306734), Anti-human CD5 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD3 BV785 (1:100, clone OKT3, BioLegend, cat # 317330), Anti-human HLA-DR BV785 (1:50 ...
• Comprehensive characterization of single cell full-length isoforms in human and mouse with long-read sequencing

  Luyi Tian, et al., bioRxiv - Genomics 2020

  Quote: ... and incubated with Fc Receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes prior to staining with TotalSeq C antibodies (Biolegend ...
• Lack of blocking activity in anti-CTLA-4 antibodies reduces toxicity, but not anti-tumor efficacy

  Erica L. Stone, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human IgG Fc allophycocyanin (APC) (BioLegend, San Diego, CA, USA) was added as a secondary antibody at 10 μg/mL and the plate was incubated at 4°C for 30 minutes ...
• Engineered niches support the development of human dendritic cells in humanized mice

  Giorgio Anselmi, et al., bioRxiv - Immunology 2019

  Quote: ... sections were labeled with mouse anti-human CD45-APC (HI30, Biolegend) for 1hr at room temperature ...
• Engineered niches support the development of human dendritic cells in humanized mice

  Giorgio Anselmi, et al., bioRxiv - Immunology 2019

  Quote: ... sections were labeled with mouse anti-human CD34-APC (561, Biolegend) for 1hr at room temperature ...
• CIS checkpoint deletion enhances the fitness of cord blood derived natural killer cells transduced with a chimeric antigen receptor

  May Daher, et al., bioRxiv - Immunology 2020

  Quote: ... Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody (HCD56, Biolegend). The following antibodies were used for intracellular staining ...