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Citations for BioLegend :
1201 - 1250 of 7888 citations for 7 CHLORO 2H PYRIDO 2 3 B 1 4 OXAZIN 3 4H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Detection of apoptotic cells was done by using FITC-Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend#640922). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice and resuspended in 300 μl of staining buffer containing 7-AAD (Biolegend, CA, USA) for flow cytometry analysis (Thermo Fisher Attune NxT - 14 color analyzer ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using anti-human/mouse □7 integrin antibody (FIB504, Biolegend) and the EasySep Biotin Positive Selection Kit (Stemcell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Organs were processed as described in 32 and samples were stained with antibodies specific for Thy1.1 (clone OX-7, BioLegend), Thy1.2 (clone 30-H12 ...
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bioRxiv - Immunology 2019Quote: 106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat ...
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bioRxiv - Cancer Biology 2021Quote: Trypsinized and washed Panc02 cells were stained with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and analyzed using the CytoflexS Flow Cytometer (Beckman Coulter).
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bioRxiv - Microbiology 2022Quote: ... Apoptotic cells were detected using the PE Annexin V Apoptosis Detection Kit with 7-AAD according to the manufacturer’s protocol (BioLegend) and analyzed using an LSRII flow cytometer.
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... Cells were then filtered through 35 μm nylon mesh and stained with Annexin V-FITC and 7-AAD (Biolegend). 50,000 cells per sample were collected for analysis using a LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... apoptosis of the Neuro-2a cell was analyzed by Annexin-V-FITC and 7-AAD staining accordingly to the manufacturer’s instruction (BioLegend). Cells were immediately acquired on a Canto II flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Cancer Biology 2023Quote: ... MEFs were trypsinized and stained with Apotracker-Green (Apo-15 peptide) and 7-AAD according to manufacturer’s instructions (Biolegend) to detect apoptotic cells ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
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bioRxiv - Immunology 2024Quote: ... purified ILC2 isolated from IL-33-challenged mice were cultured overnight with 10μM TOFA in complete media supplemented with 20 ng/ml IL-7 (Biolegend). Cells were washed with assay medium supplemented with 10 mM glucose ...
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bioRxiv - Immunology 2022Quote: ... 2 μl PE/Cyanine7 anti-human IgD antibody (BioLegend, 348210, clone: IA6-2), 0.13 μg biotinylated SARS-CoV-2 BA.2 RBD protein (customized from Sino Biological ...
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bioRxiv - Microbiology 2023Quote: ... for one hour followed by secondary antibody (anti-rabbit IgG AF555, #406412, BioLegend, San Diego, CA, USA) for 30 min at room temperature in 1X PBS/5% BSA/0.05% saponin ...
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bioRxiv - Immunology 2019Quote: ... in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Streptavidin M280 Dynabeads were conjugated to biotinylated HLA-C*05:01 refolded with P2-AV or P2-AW as described below.
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Cell Biology 2019Quote: ... with 2□mM EDTA for flow analysis or resuspended in PBS (pH 7.2) with 2□mM EDTA and 1□μg/ml DAPI (Biolegend) for sorting ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2019Quote: ... against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ: PE-Cy7, clone XMG1.2; IL2: PE, clone JES6-5H4; TNFα: APC, clone MP6-XT22; all Biolegend. all 1:200). Next ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Immunology 2021Quote: ... cells were permeabilized with 0.5% saponin for 15 min and intracellularly stained with PE-anti-perforin (clone B-D48) antibody (Biolegend) for 20 minutes ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Immunology 2022Quote: ... B cell purity was > 95% based on flow cytometric analysis following staining with anti-CD19 (Biolegend, San Diego, CA). FO and MZ B cells were >90% pure based on the criteria that they were sorted on.
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bioRxiv - Immunology 2023Quote: ... This reference curve was established in each experiment for all FcyR-specific antibodies of interest (PE-conjugated clone 10.1 to detect FcyRI, clone AT10 to detect FcyRIIa/b and clone 3G8 to detect FcyRIIIa, all from Biolegend) and used to calculate receptor numbers based on fluorescence intensity of FcyR staining on CHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β (432604) and Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Immunology 2021Quote: RAW264.7 cells of BMDMs were collected after washing in ice-cold PBS and then detaching the cells with Accutase (BioLegend). Prior to collection ...
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bioRxiv - Immunology 2021Quote: ... processing omitted the 7-AAD step and incubated with a fixable live dead dye prior to incubation with fixation buffer (Biolegend) (15 mins) ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...