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Citations for BioLegend :
1201 - 1250 of 7320 citations for 7 2 Benzylamino ethyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 2% neutral-buffered formalin diluted in FACS buffer following addition of Zombie Yellow fixable viability dye (BioLegend #423103, 1:200 in PBS).
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 μl of FACS Buffer and incubated with 1 μl of allophycocyanin (APC)-conjugated anti-CD46 antibody (BioLegend, clone TRA-2–10, 352405) for 30 min at 4°C with rotation ...
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bioRxiv - Immunology 2019Quote: ... and stained with antigen presenting cells conjugated anti-ERK1/2 pThr202/Thy204 (Biolegend, clone 6B8B69) overnight at 4°C in FOXP3 perm buffer (Biolegend) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... resuspended in 1x DAPI solution (in PBS plus 0.5% BSA and 2 mM EDTA; BioLegend) at 5×106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were subsequently blocked with 2 μL of TruStain FcX Fc receptor blocking antibody (BioLegend) for 10 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then stained with Brilliant Violent 421-conjugated ms α-EGFR (Biolegend; Table 2). Samples were gated for intact GFP+ cells and geometric mean of fluorescence intensity (geoMFI ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were incubated for 5 hours with Brefeldin A (BioLegend, San Diego, California) and Monensin (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Lung cells were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Immunology 2022Quote: A 5-plex SARS-CoV-2 Variants Neutralizing Antibody Panel (BioLegend LEGENDplex, Cat. No. #741174) was used to measure the neutralization antibody concentration ...
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Microbiology 2023Quote: ... we administered 2 μg of APC/Fire750-labeled anti-CD45 mAb (Biolegend, clone 30-F11) in mice anesthetized with an overdose of ketamine and xylazine ...
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bioRxiv - Biophysics 2023Quote: ... and 6.25 μg/mL activation antibodies against Integrin β2 (clone m24 and LFA1/2, Biolegend) were added in cell culture medium at the start of the culture.
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bioRxiv - Cancer Biology 2023Quote: ... and Jagged1/2 blocking antibodies and IgG isotype control (Biolegend, cat#s 130902, 131001, 400902) were used at 20 µM/ml.
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bioRxiv - Systems Biology 2024Quote: ... Staining was performed with FITC-coupled mouse monoclonal anti-ERK1/2 phospho (Thr202/Tyr204, Biolegend) antibody (diluted 1:200 in staining buffer ...
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bioRxiv - Immunology 2023Quote: ... The cells were then incubated overnight with rat anti-CD14 IgG (clone Sa14-2; BioLegend) and goat anti-GST IgG (Rockland ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% mercaptoethanol) supplemented with 2 μL Cell Activation Cocktail with Brefeldin A (Biolegend, #423304) and incubated for 4 h at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Each tube of cells then received 2 µL of Fc block (BioLegend, San Diego, CA), 10 µL of Brilliant Stain Buffer (Becton Dickinson) ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated in the antibody mix (2 μg/ml rat anti-CD326 (BioLegend 312502), 1 μg/ml rabbit anti-RAD51-AF647 (Bioacademia 70-012) ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained with a cocktail of cell surface antibodies (Biolegend, Table 2) in 0.5%BSA/PBS and analyzed on a LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Genomics 2019Quote: ... clones that survived after 3-4 months of culture were washed with PBS and stained with FITC anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface expression assessed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: ... IL-6 was measured using the ELISA MAX Standard Set Mouse IL-6 (Biolegend).
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bioRxiv - Microbiology 2023Quote: ... for 1h at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Immunology 2022Quote: ... and IL-6 (BioLegend, 570804). To differentiate M2 macrophages ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 6 (Biolegend, 905701), anti-pericentrin (abcam ...
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bioRxiv - Immunology 2021Quote: ... IL-6 (Biolegend, cat # 501101) and CD16/32 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... IL-6 ELISA (Biolegend, 431304); Histamine ELISA (Beckman Coulter ...
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bioRxiv - Pathology 2023Quote: ... and IL-6 (Biolegend, 431304) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... and IL-6 (BioLegend #431307) were measured by ELISA.
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bioRxiv - Immunology 2023Quote: ... anti-human IL-6 (Biolegend), or isotype control antibodies for 30 min.
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bioRxiv - Immunology 2022Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. For analyzing intracellular cytokine IFN-γ and IL-17A ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... the cells were lysed and fixed with 2 ml of 1x RBC Lysis/Fixation Solution (Biolegend) for 15 minutes at RT and washed with cold FACS-buffer (PBS with 1 % FCS) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice with 1x PBS + 2% FBS and fixed with Fluorofix Buffer (BioLegend) at RT for 30 minutes in the dark with shaking ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-human BST-2 (clone RS38E, PE-Cy7-conjugated; Biolegend, San Diego, CA, USA) were also used as primary antibodies for cell-surface staining ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with intracellular monoclonal antibody-fluorochrome conjugates: IL-2-PE (BioLegend, cat.# 500307), TNF-α-FITC (BD ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. For analyzing intracellular cytokine IFN-γ and IL-17A ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2022Quote: ... the fixed samples were stained with the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), Percp-eFluor 710-labeled iNOS (CXNFT ...
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bioRxiv - Molecular Biology 2022Quote: ... Splenocytes were stimulated for 42 hours at 37°C before adding 2 mM monensin (Biolegend #420701) for an additional 6 hours ...
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bioRxiv - Immunology 2020Quote: ... antibody was purchased from BD Biosciences (San Jose, Calif.) and the anti-CD206 antibody (clone 15-2) was purchased from Biolegend. Briefly ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Cancer Biology 2021Quote: ... then fixed in 2% PFA and permeabilized using 1X Intracellular Staining Perm Wash Buffer (BioLegend; 421002) prior to intracellular staining with Vimentin (BD Pharmingen ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. Foxp3 expression was performed using the Foxp3 staining kit from eBioscience ...
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bioRxiv - Immunology 2021Quote: ... anti-CD11c (clone N418, 25-0144-82, eBioscience) and anti-CD18 (clone M18/2, 101407, BioLegend), -CD29 (clone HMβ1-1 ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...