3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride citations for BioLegend

Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...

KLRG1 marks tumor-infiltrating CD4 T cell subsets associated with tumor progression and immunotherapy response

Casey R. Ager, et al., bioRxiv - Immunology 2023

Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...

Catabolism of extracellular glutathione supplies amino acids to support tumor growth

Fabio Hecht, et al., bioRxiv - Cancer Biology 2024

Quote: ... the pellets were incubated for 1 hour in 60 µL of staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 µL of anti-BrdU-FITC monoclonal antibody (Biolegend 364104, clone 3D4). The samples were subsequently washed with 1 mL of 1% BSA and 0.5% Tween-20 in PBS ...

Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region

S. Balasubramanian, et al., bioRxiv - Molecular Biology 2021

Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL α CD40 (BioLegend), 5 ng/ml TGF (R&D Systems ...

No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor

Jarrod Shilts, Gavin J. Wright, bioRxiv - Biochemistry 2020

Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...

Cryo-EM structures of α-synuclein filaments from Parkinson’s disease and dementia with Lewy bodies

Yang Yang, et al., bioRxiv - Neuroscience 2022

Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...

IDENTIFICATION OF A NOVEL MECHANISM FOR LFA-1 ORGANIZATION DURING NK CYTOLYTIC RESPONSE

Alejandro P. Pariani, et al., bioRxiv - Cell Biology 2022

Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...

Type 1 interferon remodels normal and neoplastic hematopoiesis in human

Chhiring Lama, et al., bioRxiv - Cancer Biology 2022

Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...

New SNCA mutation and structures of α-synuclein filaments from juvenile-onset synucleinopathy

Yang Yang, et al., bioRxiv - Neuroscience 2022

Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...

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