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1151 - 1200 of 2921 citations for Recombinant Human MAD2L1 His tagged since 2019

• Vascularised Cardiac Spheroids-on-a-Chip for Testing the Toxicity of Therapeutics

  Stefania Di Cio, et al., bioRxiv - Bioengineering 2023

  Quote: ... Alexa Fluor® 647 anti-human CD31 (BioLegend, 303112); Alexa Fluor 488 anti-hu Neural/Glial Antigen 2 (NG2 ...
• Cell-Sized Lipid Vesicles as Artificial Antigen-Presenting Cells for Antigen-Specific T Cell Activation

  Jui-Yi Chen, et al., bioRxiv - Bioengineering 2022

  Quote: ... and anti-human HLA-DR PE (BioLegend, Cat# 327007) in separate tubes ...
• Panton-Valentine leukocidin-induced neutrophil extracellular traps lack antimicrobial activity and are readily induced in patients with recurrent PVL⁺-Staphylococcus aureus infections

  Hina Jhelum, et al., bioRxiv - Immunology 2022

  Quote: ... and 10 µg/ml anti-human C5L2-APC (BioLegend) antibodies for 30 min in the dark ...
• Mitochondrial folate metabolism inhibition drives differentiation through mTORC1 mediated purine sensing

  Martha M. Zarou, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and anti– human CD38-PerCP (catalogue no.303520; Biolegend) antibodies and analysed by flow cytometry as described above.
• Dysregulated CD38 expression in blood and skin immune cells of patients with hidradenitis suppurativa

  Peter Dimitrion, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were incubated with Human TruStain FcX block (Biolegend) for 15 minutes and then were stained with cell surface antibodies for 30 minutes at RT ...
• Atypical B cells consist of subsets with distinct effector functions

  Raphael A. Reyes, et al., bioRxiv - Immunology 2023

  Quote: ... 5 µl Human TruStain FcX (Biolegend, cat. no. 422301) and 1% dextran sulfate (Sigma-Aldrich ...
• Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to vaccination

  Duncan M. Morgan, et al., bioRxiv - Immunology 2023

  Quote: ... and Total-seq A anti-human hashtag antibody (Biolegend) on ice for 30 minutes ...
• A Single-cell Perturbation Landscape of Colonic Stem Cell Polarisation

  Xiao Qin, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human TGF-β2 (1 ng mL−1, BioLegend 583301), murine TGF-β1 (1 ng mL−1 ...
• DNA methylation profiling identifies TBKBP1 as potent amplifier of cytotoxic activity in CMV-specific human CD8⁺ T cells

  Zheng Yu, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-human CD28 (CD28.2; 0.5 μg/ml; BioLegend) antibodies and cultured in TexMACS medium supplemented with 100 IU/ml human IL-2 at 37°C with 5% CO2 ...
• Immune Response of Transplanted Kidney Tissues Assembled from Organoid Building Blocks

  Thiago J. Borges, et al., bioRxiv - Bioengineering 2023

  Quote: ... and human (TruStain FcX, Fc receptor blocking solution, Biolegend) Fc-blocked for 20 min before staining for surface markers for 30 minutes in FACS buffer (2% FBS in PBS 1x ...
• Targetable leukemia dependency on noncanonical PI3Kγ signaling

  Qingyu Luo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and APC/Cyanine7 anti-mouse/human CD11b (101226; BioLegend). After staining ...
• Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)

  Maria Llamazares Prada, et al., bioRxiv - Cell Biology 2023

  Quote: ... Anti-human podoplanin (PDPN)-Alexa Fluor 647 (337007, BioLegend), Anti-human CD45-PerCP-Cy5.5 (45-9459-42 ...
• A distinctive evolution of alveolar T cell responses is associated with clinical outcomes in unvaccinated patients with SARS-CoV-2 pneumonia

  Nikolay S. Markov, et al., bioRxiv - Systems Biology 2023

  Quote: ... Fc receptors were blocked using Human TruStain FcX (Biolegend) in MACS buffer (Miltenyi Biotech) ...
• Enhanced neutralization of SARS-CoV-2 XBB sub-lineages and BA.2.86 by a tetravalent COVID-19 vaccine booster

  Xun Wang, et al., bioRxiv - Immunology 2023

  Quote: ... APC anti-human CD3 (300412, BioLegend; 1 μL/well), and PE/Dazzle™ 594 anti-human CD8 (301058 ...
• Enhanced neutralization of SARS-CoV-2 XBB sub-lineages and BA.2.86 by a tetravalent COVID-19 vaccine booster

  Xun Wang, et al., bioRxiv - Immunology 2023

  Quote: ... PE anti-human CD27 (302808, BioLegend; 1 μL/well). After being stained ...
• Generation of circulating autoreactive pre-plasma cells fueled by naïve B cells in celiac disease

  Ida Lindeman, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IgD-PerCP/Cy5.5 (clone IA6-2, BioLegend), anti-human IgD-Alexa Fluor 700 (clone IA6-2 ...
• Immune Responses in Checkpoint Myocarditis Across Heart, Blood, and Tumor

  Steven M. Blum, et al., bioRxiv - Immunology 2023

  Quote: ... Fc receptors were blocked (Human TruStain FcX, Biolegend 422302), after which cells were incubated with CD235a-PE-Cy5 (Biolegend 306606 ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD3-BV605 (Clone: OKT3; Biolegend, 317322), Verified Reactivity ...
• Loss of embryonically-derived Kupffer cells during hypercholesterolemia accelerates atherosclerosis development

  Rebecca Fima, et al., bioRxiv - Pathology 2023

  Quote: ... and rat anti-mouse/human CD324 (DECMA-1, BioLegend) antibodies ...
• Modular vector assembly enables rapid assessment of emerging CRISPR technologies

  Abby V. McGee, et al., bioRxiv - Genomics 2023

  Quote: CD97 (ADGRE5): FITC anti-human CD97 antibody (Biolegend, 336306)
• Modular vector assembly enables rapid assessment of emerging CRISPR technologies

  Abby V. McGee, et al., bioRxiv - Genomics 2023

  Quote: CD26 (DPP4): FITC anti-human CD26 antibody (Biolegend, 302704)
• The extrafollicular B cell response is a hallmark of childhood idiopathic nephrotic syndrome

  Tho-Alfakar Al-Aubodah, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IgD BV510 (1:20, IA6-2, BioLegend), anti-human IgM Alexa Fluor 488 (1:40 ...
• Generation of circulating autoreactive pre-plasma cells fueled by naïve B cells in celiac disease

  Ida Lindeman, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human CXCR5-Alexa Fluor 647 (clone J252D4, BioLegend), anti-human IgA-FITC (clone IS11-8E10 ...
• The extrafollicular B cell response is a hallmark of childhood idiopathic nephrotic syndrome

  Tho-Alfakar Al-Aubodah, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-human CXCR5 APC (1:20, J252D4, BioLegend). Intracellular proteins were stained using the following antibodies ...
• Targeting CD74-positive macrophages improves neoadjuvant therapy in cervical cancer as revealed by single-cell transcriptomics analysis

  Zixiang Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... APC anti-human CD74 Antibody (Biolegend, USA, Cat#326811) and APC anti-human CD279 (PD-1 ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD14-BV510 (Clone: M5E2; Biolegend, 301842), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD45RA-APCH7 (Clone: HI100; Biolegend, 304128), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD135-PE (Clone: BV10A4H2; Biolegend, 313306), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD3-BV605 (Clone: OKT3; Biolegend, 317322), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD56-PC5 (Clone: 5.1H11; Biolegend, 362516), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD34-BV421 (Clone: 561; Biolegend, 343610), Verified Reactivity ...
• Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)

  Maria Llamazares Prada, et al., bioRxiv - Cell Biology 2023

  Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend) for 30 minutes on ice ...
• FORMATION OF MALIGNANT, METASTATIC SMALL CELL LUNG CANCERS THROUGH OVERPRODUCTION OF cMYC PROTEIN IN TP53 AND RB1 DEPLETED PULMONARY NEUROENDOCRINE CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS

  Huanhuan Joyce Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PE anti-human CXCR4 (Biolegend, Clone 12G5, Cat# 306506) and APC anti-EpCAM (Invitrogen ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD14-PE (Clone: M5E2; Biolegend, 301806), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD56-PC5 (Clone: 5.1H11; Biolegend, 362516), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD15-APCfire750 (Clone: W6D3; Biolegend, 323041), Verified Reactivity ...
• Molecular and phenotypic blueprint of the hematopoietic compartment reveals proliferation stress as a driver of age-associated human stem cell dysfunctions

  Emanuele Lettera, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse anti-human CD34-BV421 (Clone: 561; Biolegend, 343610), Verified Reactivity ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse/human B220 APC/Cyanine7 (Biolegend, Cat: #103223), anti-mouse CD4 PerCP (Biolegend ...
• Exclusion of PD-1 from the immune synapse: a novel strategy to modulate T cell function

  Luke Yi Hao, et al., bioRxiv - Immunology 2023

  Quote: ... and human IFN-gamma kit (catalog no. 430101; BioLegend) were used according to the manufacturer protocols.
• Generation of circulating autoreactive pre-plasma cells fueled by naïve B cells in celiac disease

  Ida Lindeman, et al., bioRxiv - Immunology 2023

  Quote: ... a barcoded anti-human CD27 antibody (TotalSeq-C0154, BioLegend) was included together with fluorophore-conjugated antibodies ...
• Generation of circulating autoreactive pre-plasma cells fueled by naïve B cells in celiac disease

  Ida Lindeman, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-human CD154-APC (clone 24-31, BioLegend). Double positive cells were bulk-sorted using a FACSAriaIII instrument (BD ...
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... APC conjugated anti-human ganglioside GD2 antibody (#357306; Biolegend) or APC-Mouse-IgG2a,κ isotype control (#400220 ...
• Reproducible single cell annotation of programs underlying T-cell subsets, activation states, and functions

  Dylan Kotliar, et al., bioRxiv - Genomics 2024

  Quote: ... Staining with the TotalSeq-C Human Universal Cocktail (BioLegend) was then performed according to the manufacturer’s instructions ...
• NKp30 and NKG2D contribute to natural killer recognition of HIV-infected cells

  Nancy Q. Zhao, et al., bioRxiv - Immunology 2024

  Quote: ... and APC anti-human CD7 (BioLegend, clone CD7-6B7). For the analysis of NK cell ligand expression in CD4+ T cells or functional analysis of NK cells co-cultured with autologous CRISPR edited CD4+ T cells (Figure 4) ...
• Immunocompetent murine model of Ewing sarcoma reveals role for TGFβ inhibition to enhance immune infiltrates in Ewing tumors during radiation

  Jessica D. Daley, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Human HLA-DR Alexa Fluor 488 (Biolegend Inc 307620), Human CD16 PE/Dazzletrade 594 (Biolegend Inc 302054) ...
• The Neurolipid Atlas: a lipidomics resource for neurodegenerative diseases uncovers cholesterol as a regulator of astrocyte reactivity impaired by ApoE4

  Femke M. Feringa, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-human HLA-A,B,C Antibody (311406, BioLegend). After 3 washes in 1X PBS ...
• Aging limits stemness and tumorigenesis in the lung by reprogramming iron homeostasis

  Xueqian Zhuang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by staining with anti-human CD45 (Biolegend, #368538), CD31 (Biolegend ...
• HIV-1 neutralizing antibodies in SHIV-infected macaques recapitulate structurally divergent modes of human V2 apex recognition with a single D gene

  Ryan S. Roark, et al., bioRxiv - Immunology 2024

  Quote: ... 25 µl of Human TrueStain TcX (BioLegend, cat # 422301) was added to the cell suspension and incubated for 10 min at room temperature ...
• Infiltrating lipid-rich macrophage subpopulations identified as a regulator of increasing prostate size in human benign prostatic hyperplasia

  Nadia A. Lanman, et al., bioRxiv - Immunology 2024

  Quote: ... and blocked with Human TruStain FcX blocking antibody (Biolegend). CD45-PE [clone HI30] ...
• Gfi1 controls the formation of exhausted effector-like CD8 T cells during chronic infection and cancer

  Oluwagbemiga A Ojo, et al., bioRxiv - Immunology 2024

  Quote: ... anti-mouse/human CD44 (IM7, Biolegend, 103026, 1:200), anti-mouse CD8a (53-6.7 ...