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Citations for BioLegend :
1151 - 1200 of 3125 citations for Recombinant Human CD69 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and APC anti-human CD3 antibody (BioLegend, 300412) for 20 min ...
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bioRxiv - Genomics 2023Quote: ... APC conjugated antibodies to human CD2 (Biolegend, 300214), CD4 (Biolegend ...
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bioRxiv - Genomics 2023Quote: B2M: APC anti-human B2M antibody (Biolegend, 316312)
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bioRxiv - Cell Biology 2024Quote: ... and PE anti-human CD56 (BioLegend, cat#304606) for 45 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control ...
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bioRxiv - Cancer Biology 2024Quote: ... Human CD3 Spark Blue 550 (Biolegend Inc 344852), Human CD11b PerCP/Cyanine5.5 (Biolegend Inc 301328) ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-human CD4 AF700 (BioLegend Cat 300526), mouse anti-human CD8 PerCP (BioLegend Cat 301030) ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-human CD279 BV421 (BioLegend Cat 329920), mouse anti-human CCR7 APC-Fire 750 (BioLegend Cat 353246) ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-human CD8 PerCP (BioLegend Cat 301030), mouse anti-human CD279 BV421 (BioLegend Cat 329920) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were blocked with Human TruStain FcX (BioLegend) and stained using the listed antibodies (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2024Quote: ... Human CD19 APC/Firetrade 810 (Biolegend Inc 302272), Human HLA-DR Alexa Fluor 488 (Biolegend Inc 307620) ...
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bioRxiv - Cancer Biology 2024Quote: ... Human CD16 PE/Dazzletrade 594 (Biolegend Inc 302054), Human CD33 PE (Biolegend Inc 366608) ...
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bioRxiv - Immunology 2024Quote: ... and Human TruStain FcX Blocking Reagent (Biolegend 422302) for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... anti-human IFNγ (1 μg/mL; Biolegend, Inc) and anti-human IL-4 (2μg/ml ...
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bioRxiv - Immunology 2024Quote: ... a master mix of anti-human CD107a (Biolegend) and IL-10 catch reagent (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... PerCP/Cy5.5-conjugated anti-human IFNg (Biolegend, 506527), and APC/Cy7-conjugated anti-human TNFa (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... APC anti-human CD7 (BioLegend, clone CD7-6B7), BV605 anti-CD56 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... FITC-conjugated anti-human CD45RO (304204, BioLegend, USA), and phycoerythrin-Cy7-conjugated anti-human CCR7 (353227 ...
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bioRxiv - Cell Biology 2024Quote: ... Percp-Cy5.5 anti-human CD31 (BioLegend, cat#303132), AF-488 anti-human CD29 (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... AF-488 anti-human CD29 (BioLegend, cat#303016) and PE anti-human CD56 (BioLegend ...
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
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bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: IGSF3-Fc or normal human IgG (250 ng) and APC-conjugated anti-human IgG-Fc antibody (BioLegend, San Diego, CA, USA) was added to 1 × 105 of MS1 cells suspended in 100 µL of FACS buffer (0.5% BSA/HBSS ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated by negative selection using the EasySep Human B Cell Isolation Kit (StemCell, #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, # 480082) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... C57BL/6 or Rag2-/- mice were treated intranasally (i.n.) with 100 ng of recombinant IL-33 (Biolegend) each day for 5 consecutive days ...
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bioRxiv - Immunology 2022Quote: ... clones were screened by their loss of ability to produce IP-10 to recombinant IFNβ (Biolegend #581302).
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bioRxiv - Immunology 2021Quote: Timed-pregnant mice dams were intravenously injected with up to 10 μg of recombinant mouse cytokines (Biolegend) at gestation day 13.5 ...
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bioRxiv - Biochemistry 2021Quote: Quadruplicate samples of WT HEK293T and CCT5-FLAG tagged cells were lysed and purified using anti-FLAG affinity matrix (Biolegend cat no. 651501) using 20mM HEPES pH 7.5 and 100mM NaCl wash buffer and 200mM Glycine/150mM NaCl ...
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Cancer Biology 2019Quote: ... The TDEs were then washed and stained for intracellular cytokines using the PE/Cy7 anti-human IL-10 antibody (clone JES3-9D7) and Brilliant Violet 421™ anti-human LAP (TGF-β1) antibody (TW4-2F8) (BioLegend, CA). TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6 ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...