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Citations for BioLegend :
1151 - 1200 of 8450 citations for 4 Bromo 3' 1 3 dioxolan 2 yl 2 fluorobenzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice and resuspended in 1:4 fixation buffer (BioLegend 420801) in PBS for 10 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was discarded and the pellet was resuspended in 100µl FACS Stain Buffer (2% FBS in PBS) with 1µl anti-CD45-APC/Cy7 (clone 30-F11, BioLegend) and 1µl anti-EPCAM-AlexaFluor488 (clone G8.8 ...
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bioRxiv - Developmental Biology 2020Quote: ... Digested meniscus cells were re-suspended in flow buffer (2% FBS/PBS) and stained with Sca1 (BioLegend, 108131), Cd90 (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
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bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
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bioRxiv - Immunology 2022Quote: Whole blood inoculated with SARS-CoV-2 was blocked with 5μl of TruStain (Biolegend, San Diego, California, # 422302) for 10 minutes at room temperature and then stained for granulocyte and monocyte markers ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Immunology 2024Quote: ... Mouse and human T cells were washed three times with RPMI 10% FBS and 2.5 x 105 cells were added to each well with 2 µg/ml soluble anti-CD28 (37.51 or CD28.2, Biolegend). For co-culture experiments ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 2% PFA for 20min and permeabilized with Perm/Wash buffer (Biolegend, Cat No.421002) for 30mins ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 X10^5 cells were then resuspended to be stained with 5ul of Annexin V-FITC (#640945, Biolegend) in 100ul of Annexin V binding buffer (10mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...
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bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
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bioRxiv - Immunology 2024Quote: ... 0.25 x 106 cells were stimulated with either plate-bound 11-CD3 (BioLegend; RRID: AB_11149115; 2 µg/mL) and plate-bound 11-CD28 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The culture medium was supplemented with 10 ng/mL IL-2 and 25 ng/mL IL-15 (Biolegend). On the next day ...
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bioRxiv - Immunology 2024Quote: ... The pooled sample was then stained with diluted TotalSeq-C cell surface marker antibodies (BioLegend, Supplementary Table 2) in PBS containing 2% FBS for 30 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were then removed with a 2-minute incubation in RBC Lysis Buffer (10X) (BioLegend 420301). The solution was centrifuged again at 300 x g ...
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bioRxiv - Bioengineering 2021Quote: ... or 25 ng/ml Interleukin-4 (IL-4) (Biolegend) were used to stimulate M1 or M2 polarization ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated at +4°C with the 4G8 (Biolegend 800706, 1/500), Iba1 (Wako 1919741 ...
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bioRxiv - Systems Biology 2022Quote: ... Vimentin was performed overnight at 4°C using anti-HA (1:750, BioLegend #901502), anti-SLC1A5 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... Anti-LFA-1 antibody (M17/4) was purchased from BioLegend (San Diego, CA, USA). Fluorescence-conjugated CD3e ...
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bioRxiv - Immunology 2021Quote: ... Extracellular staining was performed in FACS buffer (PBS + 2% FBS + 2mM EDTA) with the following antibodies: CD4 (GK1.5, Biolegend), CD8 (53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Immunology 2022Quote: Serum samples were screened for neutralization using LEGENDplex SARS-CoV-2 neutralizing antibody assay (BioLegend, San Diego, CA, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2021Quote: ... 2.1 mmol/L ultra-glutamine in the presence of Brefeldin A (5 μg/ml) and monensin (2 μM; BioLegend). After 5 hours of incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 µg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Muscle sections were incubated overnight at 4 °C in blocking solution (2 % BSA, 10 % Normal Goat Serum, 0.1 % Triton and PBS) with chicken anti-NFM (#822701, BioLegend, USA ...
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bioRxiv - Immunology 2022Quote: ... followed by stimulation with plate bound anti-CD3 (2 μg/ml) and anti-ICOS (5 μg/ml; C398.4A; Biolegend) for 24 hours.
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bioRxiv - Cancer Biology 2022Quote: ... cells were pre-incubated for 2 h with the Cell Activation Cocktail containing Brefeldin A according to the manufacturer’s instructions (BioLegend). After surface staining ...
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bioRxiv - Microbiology 2020Quote: ... TNF-α and IL-2 levels were quantified in co-culture supernatants by ELISA (BioLegend, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 μg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant AAV9 (2×109 vg/well) and SpCas9 protein (0.27 μg/well) were diluted in 1x Coating Buffer A (BioLegend) and used to coat a 96-well Nunc MaxiSorp plate ...
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bioRxiv - Genomics 2021Quote: ... Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e., stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend), or DMSO as control (i.e. ...
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bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
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bioRxiv - Cancer Biology 2020Quote: ... a maximum of 2 million cells per sample was resuspended in 100 µl of cell staining buffer (Biolegend, USA) with 5 µl of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...