4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol citations for BioLegend

Quote: ... The following primary antibodies were used for immunostaining: rat anti-EpCAM-AF647 (Biolegend, clone G8.8, 1 µg ml−1, 1:250); rabbit anti-K5 (Covance ...

APOE2 gene therapy reduces amyloid deposition, and improves markers of neuroinflammation and neurodegeneration in a mouse model of Alzheimer disease

Rosemary J Jackson, et al., bioRxiv - Neuroscience 2023

Quote: ... Primary antibodies were using 1:500 rabbit anti Abeta 1:500 (IBL, CAT # 18584) and 1:500 mouse anti SMI312 (Biolegend 837904).

Innate extracellular Hsp70 inflammatory properties are mediated by the interaction of Siglec-E and LOX-1 receptors

Thiago J. Borges, et al., bioRxiv - Immunology 2023

Quote: ... IL-6 (clone MP5-20F3, 1:100), TNF-α (clone MP6-XT22, 1:66) and CD80 (clone 16-10A1, 1:100) from Biolegend.

Epithelial memory after respiratory viral infection results in long-lasting enhancement of antigen presentation

Piotr P. Janas, et al., bioRxiv - Immunology 2024

Quote: ... Fluorochrome-conjugated antibodies (1:400 anti-EpCAM-AF594, 1:200 anti-MHC-I-AF647 and 1:400 anti-MHC-II-AF488 (BioLegend, USA)) were diluted in blocking buffer and 100µl of staining mix was placed on each section for 1h at RT ...

A modiolar-pillar gradient in auditory-nerve dendritic length: a novel post-synaptic contribution to dynamic range?

Serhii Kostrikov, et al., bioRxiv - Neuroscience 2024

Quote: ... diluted in PBS with NHS and Triton X (both at 1%): 1) mouse isotype IgG2a anti-Tubulin beta 3 (TUJ1, 1:1000, BioLegend #801201), 2 ...

Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity

Zintis Inde, et al., bioRxiv - Cell Biology 2020

Quote: ... Supernatant was discarded and the pellet was resuspended in 100µl FACS Stain Buffer (2% FBS in PBS) with 1µl anti-CD45-APC/Cy7 (clone 30-F11, BioLegend) and 1µl anti-EPCAM-AlexaFluor488 (clone G8.8 ...

Identification of Gli1 as a progenitor cell marker for meniscus development and injury repair

Yulong Wei, et al., bioRxiv - Developmental Biology 2020

Quote: ... Digested meniscus cells were re-suspended in flow buffer (2% FBS/PBS) and stained with Sca1 (BioLegend, 108131), Cd90 (BioLegend ...

Early detection of cerebrovascular pathology and protective antiviral immunity by MRI

Li Liu, et al., bioRxiv - Neuroscience 2021

Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...

Histone H2Bub1 deubiquitylation is essential for mouse development, but does not regulate global RNA polymerase II transcription

Fang Wang, et al., bioRxiv - Molecular Biology 2021

Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...

Robust T cell activation requires an eIF3-driven burst in T cell receptor translation

Dasmanthie De Silva, et al., bioRxiv - Molecular Biology 2021

Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.

Multimodal Single-Cell Characterization of the Human Granulocyte Lineage

Jingjing Qi, et al., bioRxiv - Immunology 2021

Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...

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