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Citations for BioLegend :
1151 - 1200 of 1558 citations for 2 Chloro 3 6 chlorohexanoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The cells were then stained with a cocktail of cell surface antibodies (Biolegend, Table 2) in 0.5%BSA/PBS and analyzed on a LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... Western blot analysis of the samples were done using anti- HA (1:2 000; BioLegend) and anti-Flag M2 (1:5 000 ...
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bioRxiv - Immunology 2023Quote: ... The cells were then incubated overnight with rat anti-CD14 IgG (clone Sa14-2; BioLegend) and goat anti-GST IgG (Rockland ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% mercaptoethanol) supplemented with 2 μL Cell Activation Cocktail with Brefeldin A (Biolegend, #423304) and incubated for 4 h at 37 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were stained for CD69 (clone FN50, BioLegend, dilution 1:500 in PBS + 2% FBS) expression after 4 - 24 hr at 37 °C ...
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bioRxiv - Immunology 2022Quote: A 5-plex SARS-CoV-2 Variants Neutralizing Antibody Panel (BioLegend LEGENDplex, Cat. No. #741174) was used to measure the neutralization antibody concentration ...
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and Jagged1/2 blocking antibodies and IgG isotype control (Biolegend, cat#s 130902, 131001, 400902) were used at 20 µM/ml.
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bioRxiv - Immunology 2024Quote: For in vivo intravascular labelling we injected intravenously 3µg of anti-CD8β.2 FITC (Biolegend). 3 to 5 min post injection ...
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bioRxiv - Immunology 2024Quote: ... then floated on 2 μg/ml anti-Vγ5 and anti-CCR6 (BioLegend, San Diego, CA) for 1 hour at 37°C with 5.0% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... were coated overnight with purified anti-IL2 antibody (clone JES6-1A12, Biolegend, 2 μg/mL) diluted in PBS (50 μL/well) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated with 3µg/ml anti-CD3 and 3 µg/ml of anti-CD28 antibody (Biolegend clones 145-2C11 and 37.51) for 2 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... anesthetized mice (MMF i.p.) were administered with 3 µg of fluorescent-labeled anti-mouse Ly6G antibody (Alexa Fluor® 488, clone: 1A8, Biolegend, California, USA) fluorescent-labeled antibodies via i.v ...
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bioRxiv - Cancer Biology 2024Quote: ... the pellets were incubated for 1 hour in 60 µL of staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 µL of anti-BrdU-FITC monoclonal antibody (Biolegend 364104, clone 3D4). The samples were subsequently washed with 1 mL of 1% BSA and 0.5% Tween-20 in PBS ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. For analyzing intracellular cytokine IFN-γ and IL-17A ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed and fixed with 2 ml of 1x RBC Lysis/Fixation Solution (Biolegend) for 15 minutes at RT and washed with cold FACS-buffer (PBS with 1 % FCS) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice with 1x PBS + 2% FBS and fixed with Fluorofix Buffer (BioLegend) at RT for 30 minutes in the dark with shaking ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-human BST-2 (clone RS38E, PE-Cy7-conjugated; Biolegend, San Diego, CA, USA) were also used as primary antibodies for cell-surface staining ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with intracellular monoclonal antibody-fluorochrome conjugates: IL-2-PE (BioLegend, cat.# 500307), TNF-α-FITC (BD ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. For analyzing intracellular cytokine IFN-γ and IL-17A ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2022Quote: ... the fixed samples were stained with the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), Percp-eFluor 710-labeled iNOS (CXNFT ...
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bioRxiv - Molecular Biology 2022Quote: ... Splenocytes were stimulated for 42 hours at 37°C before adding 2 mM monensin (Biolegend #420701) for an additional 6 hours ...
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bioRxiv - Immunology 2020Quote: ... antibody was purchased from BD Biosciences (San Jose, Calif.) and the anti-CD206 antibody (clone 15-2) was purchased from Biolegend. Briefly ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Cancer Biology 2021Quote: ... then fixed in 2% PFA and permeabilized using 1X Intracellular Staining Perm Wash Buffer (BioLegend; 421002) prior to intracellular staining with Vimentin (BD Pharmingen ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. Foxp3 expression was performed using the Foxp3 staining kit from eBioscience ...
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bioRxiv - Immunology 2021Quote: ... anti-CD11c (clone N418, 25-0144-82, eBioscience) and anti-CD18 (clone M18/2, 101407, BioLegend), -CD29 (clone HMβ1-1 ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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bioRxiv - Cell Biology 2022Quote: ... PI (1:100) and cell hashing antibodies (1:1000, #1 for heart, #2 for blood, Biolegend) and incubated for 30min protected from light ...
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bioRxiv - Immunology 2023Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were resuspended in PBS containing 2 % BSA and a viability dye (Zombie Red, Biolegend) for discrimination between live and dead cells ...
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bioRxiv - Biochemistry 2023Quote: ... immunoprecipitation was conducted for the supernantant as described above using 2 µg of M3.2 antibody (Biolegend) and 50 µl Protein G sepharose.
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822] ...
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bioRxiv - Microbiology 2023Quote: ... 2 million PBMC of each sample were first blocked with Human TruStain FcX (BioLegend; catalog #422301), then incubated with 40ng of various anti-human hashtag antibodies carrying unique cell barcodes and 12-100ng of various CITE-seq antibodies ...
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...